Nonfermentinng gram negative bacilli Flashcards
(27 cards)
Nonfermenting gram negative bacilli
- Oxidizer or Fermenting
- TOF. Does not utilize CHO
- Aerotolerance in the environment
- The only member of the enterobacteriaceae taht is oxidase (+)
- Oxidizer/Assacharolytic
- T
- Aerobic
- Plesiomonas
Oxidase (+)
Catalase (+)
MacConkey: Gram negative bacilli
Oxidizer and assacharolytic
Pseudomonas spp
Pseudomonas spp
Morphology:
Environment:
Motile:
- Gram negative bacillus or coccobacillus
- Aerobic (strictly)
- Polar or polar flagella
- leading cause of nosocomial RTI
- 3rd most common cause of bacteremia
Pseudomonas aurigenosa
P. aurigenosa
- Presence of pus in the ear:
- Necrotizing skin rash:
- Otitis externa
- Jacuzzi or hot tub syndrome
P. aurigenosa
Virulence
- Block protein synthesis; similar to diptheria toxin
- TOF. Encapsulated.
- Mucoid caused by?
- Exotoxin A
- T
- Alginin
- proteases, hemolysins, lecithinase, elastase, DNAse
P. aurigenosa
The fluorescent group prodcues a red color pigment called?
Produced by the following?
pyoverdine (water soluble)
auruginosa, fluorescens, putida, veronii, monteilii
Other pigment:
* Blue water soluble pigment
* red
* brown
* green color
- pyocyanin
- pyorubin
- pyomelanin
- pyocyanin + pyoverdin
P. aurigenosa
- BAP:
- Odor: 2 aminoacetophenone
- Citrate:
- Growth:
- Acetamide utilization:
- Cetrimide agar growth
- ADH
- Denitrification of nitrates and nitrites
- Beta hemolytic, flat spreading colonies with a green metalic sheen
- fruity-grape or corn-taco odor
- +
- 42C
- +
- +
- +
- +
Wrinkled leathery colony
Light-yellow or brown pigment
P. stutzeri
P. stutzeri
- ADH
- Starch hydrolysis
- 6.5% NaCl
- Nitrate to nitrite reduction
- -
- +
- +
- +
● Gram (-) coccobacilli or gram (-) cocci in blood culture
● Second most common non-fermenting bacteria
● Causes 1-3% nosocomial infection next to P. aeruginosa
● Strictly aerobic
● Non-motile
● Oxidizer
● Oxidase (-)
● Catalase (+)
● Can grow in MAC
Acinetobacter spp.
Acinetobacter
- saccharolytic
- asaccharolytic
- A. baumanni
- A. lwoffi
BOLA
3rd most common non-fermentative gram (-) negative bacillus
Produces a bluish colony in MAC
STENOTROPHOMONAS MALTOPHILIA
STENOTROPHOMONAS MALTOPHILIA
Catalase
DNAse
Esculin
Gelatin hydrolysis
Lysine decarboxylase
Motile
Oxidase
- +
- +
- +
- +
- +
- +
- -
STENOTROPHOMONAS MALTOPHILIA
Drug of Choice:
Trimethoprim/sulfamethoxazole
● Motile
● Weak and Slow, Oxidase (+) positive
● ONPG and LDG (+) positive (lactose, maltose, and mannitol)
● Non wrinkled colonies differentiate it from P. stutzeri
● Produces a nonfluorescent yellow or green pigment
BURKHOLDERIA CEPACIA
BURKHOLDERIA CEPACIA
MAC
PC Agar, BCSA, OFPBL
- Pink
- Yellow
● ONLY NON-MOTILE PSEUDOMONAS
● Potential Bioterrorism Agent
BURKHOLDERIA MALLEI
BURKHOLDERIA MALLEI
- A bacterial disease of the respiratory tract and skin; RT Zoonosis (from horses, mules, and donkeys)
- Most severe disseminated type of glanders
glanders disease
farcy disease
BURKHOLDERIA MALLEI
O/F
Nitrate to nitrite
ADH
+/-
+
+
Called as “Vietnamese Time Bomb”
B. pseudomallei
B. pseudomallei
- causes a glanders like disease
- odor
- nonfermentive wrinkled colony
- O/F
- This results to a deep pink colonies from the absorption of neutral red
melioidosis
earthy
ashdown medium
+/-
colistin
TSI: K/K with H2S
Oxidase (+)
Shewanella putrefaciens
