Other fastidious organisms

Question 1

○ Not part of the normal floral cavity of humans, usually its animals.
○ Zoonotic Infection
○ Can be gotten by human via animal bite
Normal microbiota of the oral cavity of birds and mammals

Answer 1

pasteurellosis

Question 2

pasteurella spp.

• agent:
• Common specie that is common in humans

Answer 2

• shipping fever in cattles
• Pasteurella multocida

Question 3

P. multocida

1. morphology
2. oxidase
3. catalase
4. utilize
5. ornithine
6. indole
7. urease
8. BAP
9. MAC

Answer 3

1. safety pin
2. +
3. +
4. glucose
5. +
6. +
7. +
8. +
9. -

Question 4

• Ground glass or cut class
• Source of Lakes, Rivers, Hot Springs, Mud, Hot Water Systems, Water-Cooling Towers for Air-Conditioning & Evaporative Condensers
• Resist water treatment (3 mg/L chlorine)
• Adhere to pipes, rubber, plastics, and sediment
• Survive and multiply within free-living protozoa
• MOT: Aerosolized Water Particles

Answer 4

Legionnella

Question 5

Legionella

3 common species
L. pneumophilia:
L. micdadei:
L. bozemanii:

Answer 5

• Legionnaires disease & pontiac fever
• pittburgh pneumonia
• wiga’s agent of pneumonia

Question 6

Legionella

• Sputum is diluted to
• Incubate
• BAP growth
• Require
• Best medium (2)
• Colonies
• Legionella Ag
• Stain

Answer 6

• KCl-HCl
• 37C in air for at least 7 days
• negative
• I-cysteine
• BYCE with L-cysteine or Feeley gorman medium
• Grayish white or blue-green, convex and glistening colonies
• direct fluorescence antibody
• Deiterle silver stain

Question 7

Frosted glass appearance

Answer 7

Bacillus cereus

Question 8

• zoonosis- rodents, rabbits, deerfly, water trapper’s disease
• MOT: Ingestion, inhalation, arthropod bite or contact with infected tissues
• Non-motile, Capsule, Obligate anaerobe

Answer 8

Francisella tularensis

Question 9

Francisella tularensis

• Requires
• Oxidase
• Urease
• MAC
• Culture medium (3)

Answer 9

• Cysteine, cystine, or thiosulfate
• nega
• nega
• nega
• Glucose cysteine blood agar (GCBA), peptone cysteine agar, cysteine heart agar

Question 10

BORDETELLA SPP

1. Gram negative bacilli, obligate aerobe/anaerobe
2. Grows best at
3. A stain that helps identify this diagnostic feature
4. Diagnostic feature
5. Appearance

Answer 10

1. aerobe
2. 35-37
3. Toluidine blue
4. Bipolar granules
5. Pearl like

Question 11

BORDETELLA SPP

• milder form of whooping cough; pertussis-like syndrome
• Kennel’s cough (dog); Inhabits respiratory tract
  of canines; The only motile
• Causative agent of whooping cough or pertussis (Paroxysmal phase)

Answer 11

• B. parapertussis
• B. bronchiseptica
• B. pertussis

Question 12

virulence

1. B. pertussis: attachment
2. B. pertussis: interferes with signal transduction
3. B. parapertussis and B. bronchiseptica: inhibits host epithelial and immune effector cells
4. B. parapertussis and B. bronchiseptica: inhibiting DNA synthesis & promoting cell death

Answer 12

1. filamentous hemagglutinin & pertactin
2. pertussis toxin
3. Adenylate cyclase toxin
4. Tracheal cytotoxin

Question 13

Bordetella infection

1. General flu-like symptoms
2. Repetitive coughing episodes followed by the characteristic “whoop” at the end of the coughing spell
3. Recovery period

Answer 13

1. Catarrhal phase
2. Paroxysmal phase
3. Convalescent phase

Question 14

Bordetella

• Specimen
• Media (4)

Answer 14

• Nasopharyngeal aspirates or swab
• Bordet-Gengou, Regan-Lowe transport medium, Jones Kendrich, Charcoal-Cephalexin Blood Agar

Question 15

• Non-motile, Capsule, Obligate anaerobe
• Animal Abortion, Endocarditis infection

Answer 15

brucella sp.

Question 16

brucella sp.

1. enhances the growth
2. H2S ( + ):
3. dye inhibition test:
4. Serologic Test:
5. Culture Medium (4)
6. B. melitensis causing?
7. B. abortus causing?

Answer 16

• erythritol
• B. abortus, B. suis
• fuchsin and thionine dye inhibition test
• Rose bengal (+2) mercaptoethanol agglutination
• Castaneda, TSB, wisconsin, CAP
• mediterranean fever
• Bang’s bacillus