Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

DL-HP > PART 2 - HP - PT > Flashcards

PART 2 - HP - PT Flashcards

(103 cards)

Start Studying
1
Q

most informative type of microscopy because it provides ultra structural details of glomerular basement membrane?

A

EM

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

H&E Staining End-Color Results

pale pink/red/ LR

A

Proteins in Edema
Cytoplasm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

H&E Staining End-Color Results

pink?

A

Decalcified bone
Osteoid
Collagen

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

H&E Staining End-Color Results

deep pink

A

Muscle Fiber

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

H&E Staining End-Color Results

purplish pink?

A

Plasma cells
Osteoblast
Basophilic cytoplasm
Calcium
Calcified bone

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

H&E Staining End-Color Results

bright-orange to red?

A

RBC
Eosinophil granules
Keratin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

H&E Staining End-Color Results

dark blue?

A

karyosome

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

H&E Staining End-Color Results

blue to blue-black

A

nucleus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

H&E Staining End-Color Results

light blue to dark blue (ehrlicks hematoxylin)

A

cartilage

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Decontamination of SCRAPIE, what to do?

A

Treatment: Immerse the tissue for

48 hrs in FORMALIN

1 hr in FORMIC ACID

48 hrs in FORMALIN

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Accidental skin contact with hazardous chemicals, what to do?

A

wash with running tap water (15-30 mins)

never neutralize the sol’n

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Eyes splashed with chemicals, what to do?

A

go to nearest eye wash station, use eye warm bottle for 15-30 mins

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

If an important slide is broken and replacement is not available, the section (if still intact) may be

A

transfer to another slide/ mount on another slide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

it permits the entry of fluids into the cells/tses (makes tissues permeable to reagents)

A

a good fixative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Should be packed with cotton soaked in fixative or completely opened before being immersed in adequate fixing solution.

A

fixation of hollow organs: liver, stomach intestines

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

-most rapid (reduces 25-75% of impregnation time)
-impregnation under negative atmospheric pressure
-reco for urgent biopsies and delicate tissues

A

vacuum embedding

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

An element, which when added to a dye, forms a combination sometimes called a “lake” which forms a union between the stain and the tissue

A

mordant

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Metallic mordants:?

A

“TAIL-CMC”

Tungsten
aluminum
iron
lead

chromium
molybdenum
copper

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

not a Metallic mordant:?

A

iodine

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

A mordant in combination with a dye forms a?

or

it combines with the tissue to form an insoluble tse - mordant dye complex)

A

lake

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

A dye solution that is applied after the primary stain has acted

A

counterstain

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Cytoplasmic counterstains- RED?

A

“PEER”

phloxine B
eosin Y
eosin B
rose bengal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
23
Q

Cytoplasmic counterstains- YELLOW?

A

“OPO”

OG-6
Picric acid
Orange green

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
24
Q

Cytoplasmic counterstains- GREEN?

A

lissamine green
light green SF

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
25
Nuclear counterstains- RED?
"CNS" carmine Neutral red Safranin O
26
Nuclear counterstains- BLUE?
"MTCH" methylene blue toluidine blue celestine blue hematoxylin
27
most commonly used nuclear counterstain?
hematoxylin
28
most commonly used cytplasmic counterstain?
eosin Y
29
composition of Papanicolaou stain
"HOE" hematoxylin OG-6 Eosin azure
30
composition of Papanicolaou stain that stains nuclear structures?
hematoxylin
31
composition of Papanicolaou stain that stains cytoplasm of mature superficial cells?
OG-6
32
composition of Papanicolaou stain that stains cytoplasm of immature cells (Intermediate & parabasal cells)
Eosin azure
33
composition of eosin azure?
eosin Bismarck brown lithium carbonate PTA light green stain (36,50,65)
34
in Mod. Pap's, it is omitted because it stains NOTHING
Bismarck brown
35
PAPANICOLAU METHOD OF REPORTING what class? -Absence of atypical/abnormal cells -Normal Cytology -NORMAL
class I
36
PAPANICOLAU METHOD OF REPORTING what class? -atypical cytology but no evidence of malignancy -26-49% Atypical & normal cytology -(Infection or inflammation) -NORMAL & ATYPICAL
class II
37
PAPANICOLAU METHOD OF REPORTING what class? -suggestive of but NOT conclusive for malignancy -Doubtful cytology -50% undifferentiated cells (Regressive alteration of adult cell) -SUSPICIOUS
class III
38
PAPANICOLAU METHOD OF REPORTING what class? -cytology strongly -Suggestive of malignancy -Frankly malignant cytology -51-75% undifferentiated (Early stage of cancer) -SUGGESTIVE
class IV
39
PAPANICOLAU METHOD OF REPORTING what class? -cytology conclusive for malignancy -76-100% undifferentiated (With metastasis) -INDICATIVE
class V
40
Glycogen stains PAS stains what color?
magenta red bright red purple
41
Glycogen stains The PAS reaction will demonstrate fungi, because the cell wall contains
chitin
42
Glycogen stains Best Carmine stains what color?
bright red
43
Glycogen stains Control material to be used with best carmine technique can usually found in the
liver
44
Glycogen stains Langhan's lodine stains what color?
mahogany brown
45
Oil soluble dyes it has the greatest affinity to phospholipids among all the fat stains and gives triglycerides black color
Sudan black B
46
Oil soluble dyes Recommended for neutral fats (triglycerides, lipids)
sudan IV scharlach R oil red o (ORO) color: scarlet red
47
Oil soluble dyes A good stain for the CNS
sudan III
48
example of BLUEING AGENTS?
1% alcoholic ammonia (ammonia water( 1 % aqueous lithium carbonate Scott's tap water 0.2-0.5% Bicarbonate Potassium or Sodium acetate Potassium or Sodium hydroxide
49
SPECIFIC STAINS 1. it stains DNA? 2. it stains RNA?
1. methyl green (slightly acid pH) & Feulgen (sugar) 2. pyronin
50
SPECIFIC STAINS stains for muscle striations & demonstrate phosphat?
methyl green
51
SPECIFIC STAINS stains MUCIN?
-PAS -ALCIAN BLUE -METACHROMATIC DYES (Toluidine blue & azure A) -COLLOIDAL IRON
52
SPECIFIC STAINS stains reticulin fibers (argyrophilic)?
Gomori's silver impregnation
53
SPECIFIC STAINS stains elastic fibers?
orcein (brown/DB)
54
SPECIFIC STAINS most suitable for staining inflammatory cells, bacteria, blood parasites and some spirochetes
geimsa stain
55
SPECIFIC STAINS A technique for demonstrating calcium, wherein sections are immersed in silver nitrate solution are exposed to bright light
von kossa
56
SPECIFIC STAINS The pigment that is formed following a reaction of Ferrous iron from with potassium ferricyanide is known as
Turnbull's blue
57
SPECIFIC STAINS stains Ferric iron/Ferrous cyanide?
Perl's Prussian blue
58
SPECIFIC STAINS for acid mucopolysaccharides and is specific for connective tissue and epithelial mucin
alcian blue
59
SPECIFIC STAINS Purpose of crystal thymol or a few drops of chloroform in alcian blue solution?
prevent mold formation/growth of microorgs
60
SPECIFIC STAINS Staining Method for Basic Proteins?
alkaline fast green (color green) STAINS the nuleus (histones & protamines)
61
Chemical in the lab associated with bone marrow aplasia
Benzene (Clearing agent)
62
agents that are potentially explosives?
phenol picric acid silver salts sodium azide dioxane
63
Explosive when dry or mixed with metals. Toxic when absorbed through skin.
picric acid
64
Safe when fresh but explosive when old. Skin, eye and GIT irritant.
silver salts
65
Very toxic and fatal if swallowed or absorbed through the skin. Explodes upon contact with metals.
sodium azide
66
Corrosive to most metals. Should not be used in tissue processors. Skin and eye irritant and can cause severe GIT problems if ingested.
zinc chloride
67
Most commonly used FIXATIVE?
10% NEUTRAL BUFFERED FORMALIN
68
Most commonly used FIXATIVE FOR ELECTRON MICROSCOPE (EM) & HISTOCHEMISTRY
GLUTARALDEHYDE
69
Most commonly used FREEZING AGENT
LIQUID NITROGEN
70
Most commonly used DECALCIFYING AGENT
NITRIC ACID
71
Most commonly used DEHYDRATING AGENT
ETHYL ALCOHOL / ETHANOL
72
Most commonly used CLEARING AGENT
XYLENE
73
Most commonly used IMPREGNATING MEDIUM
PARAFFIN WAX
74
Most commonly used MICROTOME
ROTARY
75
Most commonly used ADHESIVE AGENT
MAYER'S EGG ALBUMIN
76
Most commonly used STAIN IN HISTOPATHOLOGY LABORATORY
HAND E STAIN
77
Most commonly used STAIN FOR CYTOLOGY
PAPANICOLAOU STAIN
78
Most commonly used MOUNTING MEDIUM
CANADA BALSAM
79
REMEDIES FOR BLACK DEPOSITS IN HISTOPATH MERCURIC CHLORIDE black deposits
Remove with ALCOHOLIC IODINE the process is called DEZENKERIZATION
80
REMEDIES FOR BLACK DEPOSITS IN HISTOPATH OSMIUM TETROXIDE black deposits
Remove with TAP WATER
81
REMEDIES FOR BLACK DEPOSITS IN HISTOPATH FORMALIN PIGMENT (Acid Formaldehyde Hematin)
Remove with PICRIC ACID (most common reagent)
82
it should not be neutralized as this may explode
Formalin
83
it is highly explosive when dry
Picric acid
84
it may explode upon air exposure
Dioxane
85
Metachromatic components?
"CECAMM" cartilage epithelial muscle connective tissue amyloid mast cell mucin
86
When examining an H&E-stained bone under the microscope, the technologist notices that there are many dark blue/purple (purplish blue) staining areas. What could this mean?
Bone was underdecalcified
87
In H and E staining, insufficient time in the acid differentiator will cause:
Under-differentiation
88
Epithelial filament markers:
Keratin Epithelial membrane antigen Carcinoembryonic membrane antigen Thyroid transcription factor-1 Prostate specific antigen
89
It is a component of B5 fixative
Mercuric Chloride
90
Antigens are most likely to be demonstrated at max sensitivity with what?
Frozen sections fixed in acetone
91
What would be proper fixation procedure for a large tissue?
Large tissue must be cut into smaller pieces
92
What is the most accurate way to determine the end point of decalcification?
Radiography
93
What is the purpose of placing the tissue ribbon in the flotation water bath?
To flatten the tissue section
94
Why should gloves be worn when cutting tissues on the microtome?
To prevent skin cells from getting on the microscope slide
95
The main difference between progressive and regression staining is the presence of?
differentiator
96
characteristics of osmium tetroxide?
Used mostly for EM Fixes lipids Commonly a secondary fixative
97
Skin tumor biopsies are preserved with what fixative?
Heidenhain's Susa
98
it is an ideal embedding medium for undecalcified bone and other hard tissue
methyl methacrylate
99
it is a popular embedding medium for light microscopy that is highly hydrophilic.
polyglycol methacrylate
100
Airholes found in the tissue during trimming is due to?
Incomplete infiltration
101
Disadvantages of celloidin impregnation?
Slow and tedious Serial sections are difficult to prepare Very thin sections are difficult to cut
102
When cutting through hard tissues such as thyroid and cervix, the ideal cutting stroke is:
Firm, quick stroke to evenly cut tse
103
A dehydrating agent that is known to be an eye and skin irritant and may cause conjunctival irritation:
Tetrahydrofuran

DL-HP (14 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions