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IMMUNE1 LAB MIDTERM AND FINALS > POST LAB EXPERIMENT 10 > Flashcards

POST LAB EXPERIMENT 10 Flashcards

(66 cards)

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1
Q

TST:

A

Direct Microscopic: Darkfield Microscopy, Fluorescent antibody testing, MHA-TP or Serodia TP- PA, DNA probe

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2
Q

NTT:

A

VDRL, RPR, TRUST

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3
Q

• Detects antibody to cardiolipin

A

Non-treponemal tests (NTT)

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4
Q

• Determine the presence of reagin

A

Non-treponemal tests (NTT)

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5
Q

• False positive results are common

A

Non-treponemal tests (NTT)

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6
Q

Non-treponemal tests (NTT)

• Antigen: (?)-used to detect the nontreponemal reaginic antibodies:
IgG or IgM class

A

cholesterol, lecithin, cardiolipin

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7
Q

• Based on flocculation reactions

A

Non-treponemal tests (NTT)

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8
Q

• NTTs are positive within (?) after the appearance of primary chancre.

A

1 to 4 weeks

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9
Q

Non-treponemal tests (NTT)

• Secondary stage-

A

False negative

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10
Q

• Titers decline in later stages

A

Non-treponemal tests (NTT)

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11
Q

• Both a qualitative and quantitative slide flocculation test for serum, and CSF

A

VDRL (Venereal Disease Research Laboratory)

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12
Q

• Serum: heated at (?) to inactivate complement and (?) is pipetted into ceramic ring of a glass slide.

A

56C for 30 minutes

0.05 mL

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13
Q

• Sera and patient samples are spread out to fill the entire ring

A

VDRL (Venereal Disease Research Laboratory)

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14
Q

• (?) of the VDRL antigen is then added to each ring.

A

One drop

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15
Q

VDRL (Venereal Disease Research Laboratory)

• Rotated for (?) on a rotator at (?)

A

4 minutes

180 rpm

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16
Q

Qualitative Serum VDRL:

A

18-gauge needle without bevel that will deliver 60 drops of antigen suspension per mL; Ring: 14mm

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17
Q

Quantitative Serum VDRL:

A

19-gauge needle without bevel that will deliver
75 drops of antigen suspension per mL

23-gauge needle that with or without bevel will deliver
100 drops of saline per mL

Ring: 14mm

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18
Q

• CSF VDRL:

A

21- or 22-gauge needle that will deliver
100 drops per mL
Ring: 16 mm

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19
Q

Read microscopically to determine the presence of flocculation or small clumps

A

VDRL

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20
Q

: the antigen volume used is less than the serum test and is at a different concentration

A

CSF

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21
Q

MAIN REACTING COMPONENT

A

CARDIOLOIPIN

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22
Q

•-ENHANCES THE REACTING SURFACE OF CARDIOLOIPIN

A

CHOLESTEROL

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23
Q

•-REMOVES THE ANTIC’ ACTIVITY OF CARDIOLOIPIN

A

LECITHIN

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24
Q

VDRL
• THE ANTIGEN IS CONSISTS OF
CARDIOLIPIN
CHOLESTEROL
LECITHIN

A

0.03%

0.9%

0.21%

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25
-involves macroscopic agglutination • Modified VDRL
RPR
26
• Cardiolipin containing antigen suspension is bound to charcoal particles
RPR
27
• The antigen is similar to the VDRL antigen with the addition of EDTA, thimerosal and choline chloride
RPR
28
•-makes the test easier to read
Charcoal
29
•: preservative
Thimerosal
30
•: inactivate the complement
Choline chloride
31
• Antigen Delivery Needle:
20-gauge disposable needle without bevel 60 drops are obtained in 1 mL Ring: 18 mm
32
RPR • Rotation:
100 RPM for 8 minutes
33
• It is a macroscopic Non Treponemal flocculation test
TRUST (Toluidine Red Unheated Serum Test)
34
• Particles of toluidine red are used in place of the charcoal particles of the RPR as the visualizing agents.
TRUST (Toluidine Red Unheated Serum Test)
35
• Quantitative, microscopic, non-treponemal.
• USR (Unheated Serum Reagin Test)
36
• Flocculation test similar to VDRL
• USR (Unheated Serum Reagin Test)
37
• The VDRL antigen is enhanced by the addition of choline chloride and EDTA.
• USR (Unheated Serum Reagin Test)
38
• Detects antibody directed against the T. pallidum organism or against specific treponemal antigens
• Treponemal Serological Tests
39
• Treponemal Serological Tests Two main types:
Fluorescent treponemal antibody absorbed test and agglutination tests
40
• Confirmation rather than screening
• Treponemal Serological Tests
41
• A dilution of heat-inactivated patient serum is incubated with a sorbent consisting of an extract of nonpathogenic treponemes (Reiter strain)
Fluorescent Treponemal Antibody Absorption Test (FTA-Abs)
42
• Slides used for this test have the Nichols strain of T. pallidum fixed to them
Fluorescent Treponemal Antibody Absorption Test (FTA-Abs)
43
- removes cross-reactivity with treponemes other than T. pallidum
• Reiter strain
44
- extracted from rabbit testicular tissue, as a substrate (antigen)
• Nichols strain
45
• Presence of fluorescence -?
Reactive
46
• Absence of fluorescence -?
Negative
47
• It is highly sensitive and specific
Fluorescent Treponemal Antibody Absorption Test (FTA-Abs)
48
• Secondary and latent syphilis-?
100% reactive
49
• Colored gelatin particles coated with treponemal antigens
Agglutination tests: Treponema pallidum Particle agglutination Tests, Hemagglutination Treponemal Test for Syphilis (TPPA)
50
• Sensitive in detecting primary syphilis
Agglutination tests: Treponema pallidum Particle agglutination Tests, Hemagglutination Treponemal Test for Syphilis (TPPA)
51
• Presence of T. pallidum antibodies are indicated by agglutination of sensitized gel particles
Agglutination tests: Treponema pallidum Particle agglutination Tests Hemagglutination Treponemal Test for Syphilis (TPPA)
52
- Antigen prepared from T. phagedenis
REITER’s ANTIGEN COMPLEMENT FIXATION TEST
53
- High proportion of false positive reactions
REITER’s ANTIGEN COMPLEMENT FIXATION TEST
54
- Gel particles or sheep red blood cells
Microhemagglutination Assay for Antibodies to Treponema pallidum (MHA-TP)
55
- Less sensitive than FTA-ABS
Microhemagglutination Assay for Antibodies to Treponema pallidum (MHA-TP)
56
Non Treponemal
SCREENING TESTS
57
Reagin
SCREENING TESTS
58
Rapid Plasma Reagin
SCREENING TESTS
59
Qualitative RPR
SCREENING TESTS
60
Quantitative RPR
SCREENING TESTS
61
Venereal Disease Research Laboratory
SCREENING TESTS
62
• Treponemal tests
CONFIRMATORY TESTS
63
• Darkfield Microscopy
CONFIRMATORY TESTS
64
• FTA-ABS
CONFIRMATORY TESTS
65
• TPPA
CONFIRMATORY TESTS
66
coated carrier particles of T. pallidum cell walls disrupted by high-frequency sound waves
Microhemagglutination Assay for Antibodies to Treponema pallidum (MHA-TP)

IMMUNE1 LAB MIDTERM AND FINALS (16 decks)

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