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IMMUNE1 LAB MIDTERM AND FINALS > POST LAB EXPERIMENT 10 > Flashcards

POST LAB EXPERIMENT 10 Flashcards

1
Q

TST:

A

Direct Microscopic: Darkfield Microscopy, Fluorescent antibody testing, MHA-TP or Serodia TP- PA, DNA probe

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2
Q

NTT:

A

VDRL, RPR, TRUST

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3
Q

• Detects antibody to cardiolipin

A

Non-treponemal tests (NTT)

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4
Q

• Determine the presence of reagin

A

Non-treponemal tests (NTT)

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5
Q

• False positive results are common

A

Non-treponemal tests (NTT)

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6
Q

Non-treponemal tests (NTT)

• Antigen: (?)-used to detect the nontreponemal reaginic antibodies:
IgG or IgM class

A

cholesterol, lecithin, cardiolipin

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7
Q

• Based on flocculation reactions

A

Non-treponemal tests (NTT)

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8
Q

• NTTs are positive within (?) after the appearance of primary chancre.

A

1 to 4 weeks

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9
Q

Non-treponemal tests (NTT)

• Secondary stage-

A

False negative

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10
Q

• Titers decline in later stages

A

Non-treponemal tests (NTT)

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11
Q

• Both a qualitative and quantitative slide flocculation test for serum, and CSF

A

VDRL (Venereal Disease Research Laboratory)

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12
Q

• Serum: heated at (?) to inactivate complement and (?) is pipetted into ceramic ring of a glass slide.

A

56C for 30 minutes

0.05 mL

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13
Q

• Sera and patient samples are spread out to fill the entire ring

A

VDRL (Venereal Disease Research Laboratory)

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14
Q

• (?) of the VDRL antigen is then added to each ring.

A

One drop

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15
Q

VDRL (Venereal Disease Research Laboratory)

• Rotated for (?) on a rotator at (?)

A

4 minutes

180 rpm

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16
Q

Qualitative Serum VDRL:

A

18-gauge needle without bevel that will deliver 60 drops of antigen suspension per mL; Ring: 14mm

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17
Q

Quantitative Serum VDRL:

A

19-gauge needle without bevel that will deliver
75 drops of antigen suspension per mL

23-gauge needle that with or without bevel will deliver
100 drops of saline per mL

Ring: 14mm

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18
Q

• CSF VDRL:

A

21- or 22-gauge needle that will deliver
100 drops per mL
Ring: 16 mm

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19
Q

Read microscopically to determine the presence of flocculation or small clumps

A

VDRL

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20
Q

: the antigen volume used is less than the serum test and is at a different concentration

A

CSF

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21
Q

MAIN REACTING COMPONENT

A

CARDIOLOIPIN

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22
Q

•-ENHANCES THE REACTING SURFACE OF CARDIOLOIPIN

A

CHOLESTEROL

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23
Q

•-REMOVES THE ANTIC’ ACTIVITY OF CARDIOLOIPIN

A

LECITHIN

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24
Q

VDRL
• THE ANTIGEN IS CONSISTS OF
CARDIOLIPIN
CHOLESTEROL
LECITHIN

A

0.03%

0.9%

0.21%

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25
Q

-involves macroscopic agglutination

• Modified VDRL

A

RPR

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26
Q

• Cardiolipin containing antigen suspension is bound to charcoal particles

A

RPR

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27
Q

• The antigen is similar to the VDRL antigen with the addition of EDTA, thimerosal and choline chloride

A

RPR

28
Q

•-makes the test easier to read

A

Charcoal

29
Q

•: preservative

A

Thimerosal

30
Q

•: inactivate the complement

A

Choline chloride

31
Q

• Antigen Delivery Needle:

A

20-gauge disposable needle without bevel

60 drops are obtained in 1 mL

Ring: 18 mm

32
Q

RPR • Rotation:

A

100 RPM for 8 minutes

33
Q

• It is a macroscopic Non Treponemal flocculation test

A

TRUST (Toluidine Red Unheated Serum Test)

34
Q

• Particles of toluidine red are used in place of the charcoal particles of the RPR as the visualizing agents.

A

TRUST (Toluidine Red Unheated Serum Test)

35
Q

• Quantitative, microscopic, non-treponemal.

A

• USR (Unheated Serum Reagin Test)

36
Q

• Flocculation test similar to VDRL

A

• USR (Unheated Serum Reagin Test)

37
Q

• The VDRL antigen is enhanced by the addition of
choline chloride and EDTA.

A

• USR (Unheated Serum Reagin Test)

38
Q

• Detects antibody directed against the T. pallidum organism or against specific treponemal antigens

A

• Treponemal Serological Tests

39
Q

• Treponemal Serological Tests Two main types:

A

Fluorescent treponemal antibody absorbed test and agglutination tests

40
Q

• Confirmation rather than screening

A

• Treponemal Serological Tests

41
Q

• A dilution of heat-inactivated patient serum is incubated with a sorbent consisting of an extract of nonpathogenic treponemes (Reiter strain)

A

Fluorescent Treponemal Antibody Absorption Test (FTA-Abs)

42
Q

• Slides used for this test have the Nichols strain of T. pallidum fixed to them

A

Fluorescent Treponemal Antibody Absorption Test (FTA-Abs)

43
Q
  • removes cross-reactivity with treponemes other than T. pallidum
A

• Reiter strain

44
Q
  • extracted from rabbit testicular tissue, as a substrate (antigen)
A

• Nichols strain

45
Q

• Presence of fluorescence -?

A

Reactive

46
Q

• Absence of fluorescence -?

A

Negative

47
Q

• It is highly sensitive and specific

A

Fluorescent Treponemal Antibody Absorption Test (FTA-Abs)

48
Q

• Secondary and latent syphilis-?

A

100% reactive

49
Q

• Colored gelatin particles coated with treponemal antigens

A

Agglutination tests: Treponema pallidum Particle agglutination Tests, Hemagglutination Treponemal Test for Syphilis (TPPA)

50
Q

• Sensitive in detecting primary syphilis

A

Agglutination tests: Treponema pallidum Particle agglutination Tests, Hemagglutination Treponemal Test for Syphilis (TPPA)

51
Q

• Presence of T. pallidum antibodies are indicated by agglutination of sensitized gel particles

A

Agglutination tests:
Treponema pallidum Particle agglutination Tests
Hemagglutination Treponemal Test for Syphilis (TPPA)

52
Q
  • Antigen prepared from T. phagedenis
A

REITER’s ANTIGEN COMPLEMENT FIXATION TEST

53
Q
  • High proportion of false positive reactions
A

REITER’s ANTIGEN COMPLEMENT FIXATION TEST

54
Q
  • Gel particles or sheep red blood cells
A

Microhemagglutination Assay for Antibodies to Treponema pallidum (MHA-TP)

55
Q
  • Less sensitive than FTA-ABS
A

Microhemagglutination Assay for Antibodies to Treponema pallidum (MHA-TP)

56
Q

Non Treponemal

A

SCREENING TESTS

57
Q

Reagin

A

SCREENING TESTS

58
Q

Rapid Plasma Reagin

A

SCREENING TESTS

59
Q

Qualitative RPR

A

SCREENING TESTS

60
Q

Quantitative RPR

A

SCREENING TESTS

61
Q

Venereal Disease Research Laboratory

A

SCREENING TESTS

62
Q

• Treponemal tests

A

CONFIRMATORY TESTS

63
Q

• Darkfield Microscopy

A

CONFIRMATORY TESTS

64
Q

• FTA-ABS

A

CONFIRMATORY TESTS

65
Q

• TPPA

A

CONFIRMATORY TESTS

66
Q

coated carrier particles of T. pallidum cell walls disrupted by high-frequency sound waves

A

Microhemagglutination Assay for Antibodies to Treponema pallidum (MHA-TP)

IMMUNE1 LAB MIDTERM AND FINALS (16 decks)

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