Practicals

Question 1

Preparation of Sickling test

Answer 1

2% sodium metabisulphite

Question 2

Principle used in Hb electrophoresis

Answer 2

Migration of charged solutes or particles in liquid medium under electric field based on the size and the shape of the particle and the strength of the electric field

Question 3

Positive substances moves towards the

Answer 3

Cathode (neg)

Question 4

Negative substance moves towards the…

Answer 4

Anode

Question 5

Type of electrophoresis used in Ghana

Answer 5

Cellulose acetate paper or membrane electrophoresis

Question 6

Ph medium of Hb electrophoresis

Answer 6

8.2-8.6

Question 7

Hb migrates towards cathode or anode

Answer 7

Anode

Question 8

Substance used to lyse cells in Hb electrophoresis

Answer 8

Saponin or freezing

Question 9

Solubility test principle

Answer 9

Rbc lysed by saponin
Hb reduced
If HbS, low solubility in reduced form and will form crystals leading to turbidity

Question 10

What principle is used in fetal hemoglobin determination

Answer 10

More sensitive to Alkaline dénaturation so alkaline solution added to Hb and amount of Hb desaturated calculated by colorimètry