SECTION CUTTING

Question 1

Process whereby tissues are cut into uniformly thin slices or “sections” with the aid of a microtome, to facilitate microscopic study

Answer 1

CUTTING OF SECTIONS

Question 2

Where the tissue is held in position

Answer 2

Block holder

Question 3

For actual cutting of tissue sections

Answer 3

Knife carrier and Knife

Question 4

To line up the tissue block in proper position with the knife, for adjusting the proper thickness of the tissue

Answer 4

Pawl, Ratchet feed wheel and Adjustment Screws

Question 5

Simplest type. Serial sections of small and large blocks of paraffin embedded tissues

Answer 5

Rocking/Cambridge

Question 6

Who introduced Rocking/Cambridge

Answer 6

Paldwell-Trefall

Question 7

Section thickness using Cambridge microtome

Answer 7

10-12 µ thick

Question 8

COMMONLY USED FOR ROUTINE AND RESEARCH LABORATORIES

Answer 8

Rotary/Minot

Question 9

Heavier and more stable than Rocking Microtome

Answer 9

Rotary/Minot

Question 10

MOST COMMONLY USED FOR ROUTINE PARAFFIN EMBEDDED SECTIONS

Answer 10

Rotary/Minot

Question 11

Who introduced Rotary/Minot

Answer 11

Minot

Question 12

Section thickness using Minot microtome

Answer 12

4-6 µ thick

Question 13

For celloidin embedded sections

Answer 13

Sliding microtome

Question 14

MOST DANGEROUS TYPE OF MICROTOME

Answer 14

Sliding microtome

Question 15

Has 2 movable pillars that holds the adjustable knife clamps

Answer 15

Base Sledge Sliding

Question 16

Who introduced Sliding microtome

Answer 16

Adams

Question 17

Section thickness using Sliding microtome

Answer 17

7-9 µ thick

Question 18

Unembedded frozen sections

Answer 18

Freezing microtome

Question 19

Who introduced Freezing microtome

Answer 19

Queckette

Question 20

Section thickness using Freezing microtome

Answer 20

10-15 µ thick

Question 21

Electron microscopy

Answer 21

Ultrathin Sectioning

Question 22

Section thickness using Ultrathin Sectioning

Answer 22

0.5 µ thick

Question 23

Apparatus capable of freezing the tissue into the blockholder to the correct degree of hardness to facilitate easier and faster sectioning

Answer 23

Cold/Cryostat Microtome

Question 24

Fluorescent Ab Staining; Fresh Tissue Examination; Urgent Biopsies

Answer 24

Cold/Cryostat Microtome

Question 25

Cold/Cryostat environment is kept at what temperature

Answer 25

-5 to -30°C (average -20°C)

Question 26

Cold/Cryostat Microtome is capable of freezing fresh tissues within

Answer 26

2-3 minutes

Question 27

Paraffin sections are cut between

Answer 27

4-6 µ thick for routine procedures

Question 28

Knife is usually tilted at ____ angulation on a microtome to allow a clearance angle between the cutting facet and the tissue block

Answer 28

0-15°

Question 29

Temperature of water bath

Answer 29

6-10°C lower than the melting point of the wax used for embedding the tissue

Question 30

The process of lifting out tissue sections from water bath

Answer 30

FLOATING-OUT or FISHING-OUT

Question 30

The purpose of floating out bath for tissue ribbon is

Answer 30

To flatten folds and creases

Question 31

Tissue sections are then placed in a paraffin oven (maintained at a temperature _____ the melting point of paraffin wax) to dry

Answer 31

2-5°C above

Question 32

Celloidin embedded tissue blocks which may be cut by sliding microtome, sections are cut between

Answer 32

7-9µ thick/ >8µ

Question 33

May be cut from fresh or fixed tissues frozen with CO2, and to cut in a freezing microtome or Cryostat

Answer 33

FROZEN SECTIONS