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HISTOPATHOLOGY > CLEARING/DEALCOHOLIZATION > Flashcards

CLEARING/DEALCOHOLIZATION Flashcards

1
Q

Process whereby alcohol is removed from the tissue and replaced with a substance that will dissolve the wax with which the tissue is to be impregnated

A

CLEARING

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2
Q

Commonly used clearing agents:

A

Xylene, toluene, benzene, chloroform, cedarwood oil, aniline oil, clove oil and CCI4

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3
Q

Clearing agents should be miscible with alcohol, paraffin and/or mounting medium. True or False?

A

True

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4
Q

Urgent biopsies; carcinogenic: bone marrow damage and aplastic anemia

A

Benzene

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5
Q

Tissue:Clearing agent ratio

A

1:10

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6
Q

MOST COMMON; embedding and mounting procedures

A

Xylene (Xylol)

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7
Q

Clearing time of Xylene

A

15mins-1hour

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8
Q

Routine post-mortem research tissues

A

Toluene

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9
Q

Clearing time of Toluene

A

1-2hours

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10
Q

Urgent biopsies; carcinogenic: bone marrow damage and aplastic anemia

A

Benzene

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11
Q

Clearing time of Benzene

A

15mins-1hour

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12
Q

CNS tissues, smooth muscles, skin, and cytological studies

A

Cedar Wood Oil

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13
Q

Clearing time of Cedar Wood Oil

A

Paraffin sections: 2-3 days
Celloidin sections: 5 -6 days

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14
Q

Lymph nodes, embryo, nervous tissue

A

Chloroform

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15
Q

Clearing time of Chloroform

A

6-24hours

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16
Q

Embryos, insects, and very delicate specimens

A

Aniline Oil

17
Q

Slow, minimum shrinkage tissues, adultered tissue

A

Clove Oil

18
Q

For embedding process; similar to chloroform

A

Carbon Tetrachloride

19
Q

Dehydrating and clearing agent

A

Tetrahydrofuran and Dioxane

20
Q

Substitute of Cedar wood oil

A

Terpinol

21
Q

Large pieces of tissues and embryonic materials

A

Amyl acetate

HISTOPATHOLOGY (16 decks)

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