Serology - Basic

Question 1

1. False positive results when using cassettes may result from:

Answer 1

Under centrifugation

Fibrin clots in serum

Question 2

In processing EIA, arrange the following steps chronologically: a) Calculation of CO( cut off) b) Dispensing stop solution. c) Read the optical density d) interpretation of results

Answer 2

Dispensing stop solution. 1
Read the optical density 2
Calculation of CO value ( cut off) 3
interpretation of results 4

Question 3

While performing semi automated EIA:

Answer 3

Use a new tip for each sample.

Question 4

False negative results when using cassettes may result from:

Answer 4

Over centrifugation

Question 5

Discrepancies between forward & reverse ABO grouping could be resolved through :

Answer 5

Repeating the test & Antibody screening & Allowing more incubation

Question 6

Documentation in Grouping Lab include all the following except

Answer 6

Stock cards

Temperature monitoring charts

Maintenance and calibration charts

Quality Control monitoring charts

Except

EIA plate monitoring sheet

Question 7

Values of the negative and positive controls should follow the test validity conditions:

Answer 7

True

Question 8

Discrepancies between forward & reverse ABO- blood grouping may occur due to:

Answer 8

Procedural errors
A & B subgroups
Irregular antibodies in patient’s serum

Question 9

Disadvantages of slide technique for blood grouping include:

Answer 9

Missing of weak expressions of A & B antigens
Missing of weak/variant D antigen
Not suitable for reverse grouping

Question 10

Maintenance of the equipment in the lab is the responsibility of:

Answer 10

Staff
Biomedical engineering department
Supplier