- Aerobic - Anaerobic - Yellow - Light Blue - Red - Orange - Gold - Light Green - Lime - Green - Lavender - Pink - Royal Blue - Tan - White - Grey - Black

Specimen Procurement, Processing & Data Collection Flashcards by Courtney Ormerod

Order of Draw

Aerobic
Anaerobic
Yellow
Light Blue
Red
Orange
Gold
Light Green
Lime
Green
Lavender
Pink
Royal Blue
Tan
White
Grey
Black

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Additives: Aerobic

Culture

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Additives: Anaerobic

Culture

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Additives/Inversions: Yellow (Sterile)

SPS (Sodium Polyanethol Sulfonate
8x

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Additives/Inversions: Yellow (Non-Sterile)

Acid Citrate Dextrose
8x

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Additives/Inversions: Light Blue

Sodium Citrate:
- Discard tube first to get rid of air bubbles
- DO NOT UNDERFILL
3-4x

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Additives/Inversions: Red

None
0x

Clot Activator
5x

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Additives/Inversions: Orange

Thrombin
8x

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Additives/Inversions: Gold

SST (Serum Separating Tube):
- Thixotropic gel
5x

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Additives/Inversions: Light Green

PST (Plasma Separating Tube):
- Lithium Heparin
8x

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Additives/Inversions: Lime

Barricore

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Additives/Inversions: Dark Green

Sodium Heparin
8x

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Additives/Inversions: Lavender

EDTA
8x

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Additives/Inversions: Pink

EDTA K2:
- Spray-coated
8x

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Additives/Inversions: Royal Blue

EDTA

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Additives/Inversions: Tan

EDTA
8x

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Additives/Inversions: White

EDTA

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Additives/Inversions: Grey

Sodium Fluoride
8x

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Additives/Inversions: Black

Sodium Citrate
10x

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What is Plasma?

Unclotted liquid component of blood

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What is Serum?

Clotted liquid component of blood

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Collection Type: Arm

Antecubital Fossa:
- Median cubital (middle)
- Cephalic (outer side of arm)
- Brachial (inner side of arm)

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Collection Type: Hand

Butterfly/winged infusion

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Collection Type: Finger

Microtainer or Lancet
(wipe away first drop)

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Collection Type: Heel

- Prewarm - Never more than 2mm depth - Lateral heel

Needle Types: Green

21g - Used mainly for patients on blood thinners

Needle Types: Black

22g - Standard

Needle Types: Blue

23g

Lancet Types: Blue

Adult

Lancet Types: Pink

Neonate or Geriatric

Special Considerations: Edema

Pressure to move fluid away from tourniquet/puncture site or select other side

Special Considerations: Hematoma/Bruising

Select other side

Special Considerations: IV Placement

- Opposite arm or - IV off for 5 mins - Select site distal to IV - Select different vein

Special Considerations: Masectomy

Opposite side of the body

Special Considerations: Petechiae

Red spots form, from too tight/too long tourniquet

Special Considerations: Blood Alcohol Testing

Use chlorhexidine, NOT alcohol based cleansers

Special Considerations: Hemolysis

Affects: - K (potassium) - LD (lactate dehydrogenase) - AST (liver function) - Mg (magnesium) - Iron - B12 - Folate

First Aid: Fainting

- Remove tourniquet and needle - Cap needle asap and safely - Tend to patient

First Aid: Hematoma/Extended Bleeding

Pressure for 5 minutes then check

First Aid: Tourniquet

No longer than 1 min

First Aid: CAB

C - Compressions A - Airway B - Breathing

Specimen Storage: Light Sensitive

- Bilirubin - Erythrocyte Protoporphyrin - Carotene - Vitamin A

Specimen Storage: Warm

- Cold Agglutinin - Cryoglobulin

Specimen Storage: Chilled

- ACTH (adrenocorticotropic hormone) - Acetone - ACE (angiotensin converting enzyme) - Angio - Aldosterone - Ammonia - Catecholamine - Free Fatty Acids - Lactate - Pyruvate - Renin Activity - DHEA (dehydroepiandrosterone) - Vitamin C

Chain of Custody

- Record every person that touches specimen - Ensure seal/paperwork

Precautions: Contact

Infection spread by touch

Precautions: Droplet

Infection spread by cough/sneeze particles

Precautions: Airborne

Infection spread in the air

Precautions: Nosocomical

Hospital acquired infection (HAI)

Additives: EDTA

- Maintains cell shape - Prevents clotting by binding with calcium

Additives: Heparin

Inactivates thrombin and factor Xa

Additives: Sodium Citrate

- Mild reaction binding with calcium - Preserves factor V

Additives: Sodium Fluoride

Preserves glucose

Storage: Fungal Cultures

Stored at RT (room temp)

Storage: Blood/CSF

Can be stored at 35C (body temp)

Storage: Urine

Stored at 4C

Assorted Acronyms: GTT

Glucose tolerance test

Assorted Acronyms: OGTT

Oral glucose tolerance test

Assorted Acronyms: LH

Luteinizing Hormone

Assorted Acronyms: PSA

Prostate Specific Antigen

Assorted Acronyms: QNS

Quantity not Sufficient

Assorted Acronyms: ETS

Evacuated Tube System

Assorted Acronyms: BT

Bleeding Time

Assorted Acronyms: CBC

Complete Blood Count

Assorted Acronyms: ACD

Acid Citrate Dextrose

Assorted Acronyms: CK

Creatine Kinase

Assorted Acronyms: TSH

Thyroid Stimulating Hormone

Assorted Acronyms: PP

Post Prandial

Assorted Acronyms: PLT

Platelets

Assorted Acronyms: XDP

Serum Crosslinked Fibrin

Assorted Acronyms: HCT

Hematocrit

Assorted Acronyms: BBP

Blood Borne Pathogen

Assorted Acronyms: ESR

Erythrocyte Sedimentation Rate