Topic 6 -3 Flashcards
(10 cards)
What technique is used to isolate individual species from a mixed culture of bacteria?
Streak plating
Streak plating is a method that allows for the isolation of individual bacteria from a mixed culture by spreading a culture thinly on an agar plate.
What is the purpose of measuring the rate of growth of bacteria in liquid culture?
To determine bacterial population growth
Measuring growth rates helps in understanding the dynamics of bacterial populations and their responses to environmental conditions.
What is the first step in the streak plating process?
Use a sterile inoculating loop to spread a drop of a bacterial culture
This step is crucial to avoid contamination and to ensure that bacteria are spread thinly.
What is the outcome of spreading bacteria thinly in streak plating?
Individual bacteria grow into separate colonies
This allows for the isolation of specific bacterial species from a mixed culture.
What must be kept constant when measuring bacterial growth rates?
Temperature
Maintaining a constant temperature is important for accurate and consistent growth measurements.
What technique can be used to count bacteria in a sample?
direct counting, dilution plating, or using a hemocytometer.
What is done with the initial sample in a bacterial growth investigation?
Count bacteria
Counting the initial sample provides a baseline for measuring growth over time.
What type of broth is used in the bacterial growth investigation?
Nutrient broth
Nutrient broth provides essential nutrients for bacterial growth.
Fill in the blank: In streak plating, bacteria are _______ to distribute them evenly.
shaken
Shaking helps to evenly distribute the bacteria in the nutrient broth before plating.
What can the collected data from bacterial growth investigations be used to calculate?
Rates of growth and the exponential growth rate constant
These calculations are essential for understanding the growth dynamics of the bacterial species studied.
