U3 LAB: MICROSCOPIC MORPHOLOGY Flashcards by maki 🐈‍⬛

Uses one stain

Simple stain

How well did you know this?

Not at all

Perfectly

Provides morphology and arrangement

Simple stain

How well did you know this?

Not at all

Perfectly

Example of simple stain

Cocci in chains

How well did you know this?

Not at all

Perfectly

Commonly used in Bacteriology

Differential stain

How well did you know this?

Not at all

Perfectly

Provides Gram reaction, morphology, and arrangement

Differential stain

How well did you know this?

Not at all

Perfectly

Primary stain

Crystal violet

How well did you know this?

Not at all

Perfectly

Mordant

Gram’s iodine

How well did you know this?

Not at all

Perfectly

Use of mordant

enhances primary stain to organism

How well did you know this?

Not at all

Perfectly

Decolorizer

acetone alcohol

How well did you know this?

Not at all

Perfectly

Use of decolorizer

removes primary stain

How well did you know this?

Not at all

Perfectly

Secondary stain

Safranin

How well did you know this?

Not at all

Perfectly

No mordant

secondary stain will be observed (pink)
- erroneous result
- false negative result

How well did you know this?

Not at all

Perfectly

No alcohol

purple
- false positive result

How well did you know this?

Not at all

Perfectly

Preparation of smear from clinical specimens

Direct smear

How well did you know this?

Not at all

Perfectly

Cells observed in Direct Smear

Bacterial cells
RBCs, WBCs

How well did you know this?

Not at all

Perfectly

Preparation of smear from culture

Indirect smear

How well did you know this?

Not at all

Perfectly

Comma shaped bacilli

Vibrio

How well did you know this?

Not at all

Perfectly

Not usually encountered, gram stain is not commonly done

Spiral

How well did you know this?

Not at all

Perfectly

Gram (+) cocci in tetrads

Micrococcus tetragena

How well did you know this?

Not at all

Perfectly

Gram (+) cocci in pairs (lancet shaped)

Streptococcus pneumoniae

How well did you know this?

Not at all

Perfectly

Gram (-) cocci in pairs (may be found intracellularly inside PMN, coffee bean shaped)

Neisseria sp. (N. meningitidis, N. gonorrhoeae)

How well did you know this?

Not at all

Perfectly

Gram (+) cocci in sarcinae (cubical packet)

Sarcina lutea

How well did you know this?

Not at all

Perfectly

Gram (+) cocci in chains

Streptococcus

How well did you know this?

Not at all

Perfectly

Gram (+) cocci in clusters

Staphylococcus

How well did you know this?

Not at all

Perfectly

Gram (+) bacilli in chains (large bacilli with square cut ends)

Bacillus

Bamboo fishing pole appearance

Bacillus

Clear structures in Bacilli

Endospores

Gram (-) bacilli in pairs

Pseudomonas or non-fermenters

Refers to elongated cocci

Coccobacilli

Gram (-) coccobacilli in pairs, singles

Enterics

Gram (+) bacilli that are large, arranged singly or inidividually with spores

- Clostridium tetani - Clostridium botulinum

Spore of C. tetani is located?

terminally

Box car appearance

Clostridium perfringens

Gram (-) coccobacilli straight and rod shaped

Haemophilus ducreyi

- smallest pathogenic bacilli - school of fish / railroad track pattern

Haemophilus ducreyi

Gram (-) bacilli with rounded ends

Bacteroides fragilis

Gram (+) large irregular bacilli

Corynebacterium

"club shaped" , Chinese Character, X or Y, palisade, snapping arrangement

Corynebacterium

Gram (+) branching bacilli with bifurcated or spatulated ends

Bifidobacterium

Gram (+) slender, branching, filamentous bacilli

Nocardia

T/F: Nocardia is also partially acid fast.

True

Gram (+) filamentous bacilli with a V-shaped pattern

Erysipelothrix rhusiopathiae

Erysipelothrix rhusiopathiae is the causative agent of?

Red disease

Gram (-) bacilli in long looped filamentous forms

Streptobacillus moniliformis

Streptobacillus moniliformis is also known as?

necklace bacillus

Gram (-) fusiform bacilli

Fusobacterium nucleatum

Gram (-) comma/curved shaped bacilli in singles

Vibrio

Gram (-) seagull wing shaped, S shaped bacilli

Campylobacter

Gram (-) curved bacilli with two or more curves

Spirillum

On gram stain, AFB is?

weakly gram positive

Acid Fast Bacilli Slender Serpentine cord like pattern

Mycobacterium tuberculosis

AFB Primary stain

Carbol fuchsin

AFB Mordant

Heat / Tergitol

AFB Decolorizer

Acid alcohol

AFB Secondary stain

Methylene blue / Malachite green

Acid Fast Bacilli Slender Palisades / globi packets / cigar bundle, arranged singly

Mycobacterium leprae

M. leprae is a causative agent of?

Leprosy

What microscope is used for Spirochetes?

Darkfield microscopy

What stains are used for Spirochetes?

- silver stains - fluorescent stains - tissue stains (Giemsa)

Tightly twisted, with one end or both bent to a hook

Leptospira interrogans

Leptospira interrogans resemble a?

Question mark

Motility of T. pallidum

Corkscrew motility

Flexibly twisted

Borrelia

This is the only spirochete that can be stained with Giemsa

Borrelia

Yeast cells are Gram ___

positive

Square shaped bacteria

Haloarcula spp.

Starshaped bacteria

Stella spp.

DSSM stands for?

Direct Sputum Smear Microscopy

What should be read before reporting sputum smear negative for ABF?

300 OIF

Structures seen in sputum smear

- WBC (pus cells) - Squamous epithelial cells - Mucus threads

Spiral shaped structures that can be seen in asthmatics

Curschmann's Spirals