U3 LEC: BACTERIAL CULTIVATION Flashcards
(93 cards)
1
Q
Microscopic examination of specimens provide preliminary information about a bacterial infection.
A
Bacterial Cultivation
2
Q
Part of Koch’s postulate
A
What is seen in sample microscopy should be observed in sample culture
3
Q
This provides definitive ID of a particular agent
A
Cultivation and isolation
4
Q
Flow of Traditional ID
A
- Sample collection
2, Microscopy (stained/unstained) - Culture
- Microscopy of Culture
5
Q
Flow of Alternative Bacterial ID
A
- Sample collection
- Culture
- Molecular testing
6
Q
Normal flora of the throat
A
Moraxella catarhalis
7
Q
One of the most sophisticated biochemical testing, just highly presumptive (not definitive and confirmatory)
A
VITEK
8
Q
VITEK is best for?
A
Drug Susceptibility and Antimicrobial Susceptibility Testing
9
Q
Purposes of bacterial cultivation
A
- grow and isolate all bacteria
- determine bacteria that are most likely causing infection
- obtain sufficient growth of clinically relevant bacteria
10
Q
In vitro
A
artificial
11
Q
In vivo
A
animals or human
12
Q
In situ
A
environment
13
Q
Key Terms
To introduce
A
Inoculation
14
Q
Key Terms
Media that has the organism
A
Inoculum
15
Q
Other Key Terms
A
- Incubation
- Cultures
- Colonies
- Isolation
16
Q
Criteria for Growth Media
A
- sufficient moisture/water
- properly adjusted pH
- suitable level of oxygen
- sterile
- incubated at proper temp
- free from inhibitory substances
- must contain essential nutrients in proper concentration
17
Q
Classification of Growth Media: According to Physical state
A
- Liquid
- Semi-solid
- Solid
- Biphasic media
18
Q
This media can be observed in the blood culture bottle.
A
Biphasic Media
19
Q
This is the first solidifying agent used.
A
Gelatin
20
Q
At this percent, it can be used to detect presence of gelatinase.
A
12%
21
Q
Red algae
A
Rhodophyta
22
Q
Characteristics of Solid Media
A
- not degradable
- liquid at 55-60C
- solid at 50C or lower
- melts at high temperature (95-100C; Hysteresis)
23
Q
According to dispensing/formation
A
- Plated medium
- Tubed
- Bottled
- Biphasic
24
Q
Proper form of dispensed media
A
smooth, equal, molten liquid, no bubble formation, not too thin or thick
25
Kirby-Bauer Method
Mueller-Hilton Agar: neutral pH, 0.5 McFarland Standard, 4mm thick
26
This media can be used 5 days from preparation, prone to drying and contamination.
Plated Medium
27
T/F: If there is still liquid in plated media, you can still use it.
True
pwede pa basta moist environment
28
Screw Cap tubes lasts up to?
2 weeks to 1 month
29
Tubes with cotton lasts up to?
2 weeks
30
Matter of Dispensing
Plated
1. Weigh
2. Dissolve
3. Autoclave
4. Dispense
31
Matter of Dispensing
Tubed
1. Weigh
2. Dissolve
3. Dispense
4. Autoclave
32
Manner of Formation
Plated Medium
- Clock Method
- Radial
- Simple
33
Manner of Formation
Butt
Stab
34
Manner of Formation
Slant
Streak
35
T/F: Spreading is equal to seeding, but not equal to streaking.
True
36
Manner of Formation
Few growth in primary streak
very little growth
37
Manner of Formation
Growth only in primary streak
Little growth
38
Manner of Formation
Growth in primary and secondary streak
Moderate growth
39
Manner of Formation
Growth in primary, secondary, tertiary streak
Heavy growth
40
Manner of Formation
1 to 10 colonies only
Rare growth
41
According to Composition
- Chemically Defined / Synthetic
- Complex / Non-chemically defined
- Tissue Culture
42
According to Composition
Has defined compositions and concentrations
Chemically defined / Synthetic
43
According to Composition
Cannot be defined
Complex / Non-chemically defined
44
According to Composition
Examples of Complex / Non-chemically defined
Blood agar, Chocolate agar
45
According to Composition
This refers to organized tissues to look like organs, used for cancer treatment and bacterial culture
Organoids
46
According to function / use
- Basic / Basal / General Purpose
- Enriched Culture Media
47
According to function / use
Supportive media, for non-fastidious organisms
Basic/Basal/General Purpose
48
According to function / use
Fortified with nutrients, can grow some fastidious organisms
Enriched Culture Media
49
According to function
- Selective medium
- Differential medium
50
According to function
Refers to inhibitors
Selective Medium
51
According to function
Refers to inhibitors and indicators
Differential
52
According to function
This can differentiate one colony from the other
Indicators
53
Black colonies, hydrogen sulfide indicator
Salmonella agar
54
No hydrogen sulfide indicator
Shigella agar
55
Inhibitors for Gr (+) bacteria
- Dyes (gentian violet, crystal violet)
- Bile Salt (Sodium Desoxycholate)
56
Has crystal violet, selective for Gr (-) bacteria, differentiate for lactose and non-lactose fermenters
MacConkey Agar
57
Selective media for Staphylococcus sp.
Mannitol Salt Agar
58
According to function
This causes growth of enteric pathogens but will inhibit growth of Gr (+) bacteria
Bile Salt (Sodium Desoxycholate)
59
Inhibitors for Gr (-) bacteria
- Sodium azide
- Potassium tellurite
60
According to function
This is the best preservative for urine samples
Sodium azide
61
Selective Culture Media
For TB
Lowenstain Jensen Medium
62
Selective Culture Media
Lowenstain Jensen Medium is enriched in?
Albumin
63
Selective Culture Media
Inhibitor in Lowenstain Jensen Medium
Malachite green
64
Selective Culture Media
For Gram Positive (+)
Phenylethyl alcohol
65
Selective Culture Media
For Neisseria gonorrhoeae
- Thayer Martin
- Modified Thayer Martin
- Martin Lewis
- New York City Agar
66
Selective Culture Media
Inhibitor for Thayer Martin
- Vancomycin (Gr +)
- Colistin (Gr -)
- Nystatin (fungi)
67
Selective Culture Media
Inhibitor for Modified Thayer Martin
addition of Trimethoprim lactate
68
Selective Culture Media
Inhibitor for Martin Lewis
- Vancomycin
- Colistin
- Trimethoprim lactate
- Anisomicin (fungi)
69
Selective Culture Media
Inhibitor for New York City Agar
- Amphotericin B (fungi)
70
Mannitol Salt Agar
Inhibitor
7.5% NaCl
71
Mannitol Salt Agar
pH indicator
Phenol Red
72
Mannitol Salt Agar
Normal color, and color change
Normal: pink
Change: yellow
73
Thiosulfate Citrate Bile Salts Sucrose Agar
Inhibitor
- Thiosulfate
- Bile salts
- high pH
74
Thiosulfate Citrate Bile Salts Sucrose Agar
pH indicator
Bromthymol blue
75
Thiosulfate Citrate Bile Salts Sucrose Agar
Color of colonies
yellow and green
76
According to use
- Enrichment medium
- Transport medium
- Characteristic / biochemical characteristic medium
- Motility medium
- Antibiotic susceptibility
- Anaerobic / Reducing culture medium
- Pharmaceutical agar
- Media for capnophiles
77
According to use
Increases number of pathogens
Enrichment medium
78
According to use
Maintain viability
Transport Medium
79
According to use
Under enrichment medium
- Alkaline peptone water
- Tetrathionate
- Hafnia or gram negative broth
80
According to use
Under transport medium
- Amies, Cary-blair (Stool)
- Transgrow
81
According to use
Under biochemical
- Triple Sugar Iron Agar (Gram - bacteria; ability to ferment glucose, lactose or sucrose to produce H2S and gas)
82
According to use
under Motility medium
Sulfide Indole Motility (SIM)
83
According to use
under Antibiotic susceptibility
- Mueller Hinton Agar
- Middle Brook Agar (need high protein)
84
According to use
under Anaerobic
Bacteroides anaerobic medium (Additives that reduce O2)
85
According to use
under Pharmaceutical
research, no nutrients, pure agar
- Malunggay leaves
- Malco agar (coconut water)
86
Manifestations of Growth
Solid Media
Appearance of colonies
87
Manifestations of Growth
Liquid media
- Turbidity
- Presence of Pellicle
- Clot or coagulation
- Bubbles
88
Location in broth media
- Surface
- Column
- Bottom
89
When should you observe colony morphology?
18 to 24 hours postculture
90
This may affect size and characteristics of colonies
Age of culture
91
This determines the growth and colony morphology.
Plating media
92
Other term for plating media
Plate reading
93
T/F: Fastidious organisms require a longer time of incubation.
True
