U3 LEC: COLONIAL MORPHOLOGY

Question 1

Importance of Colonial Morphology as a Diagnostic Tool

Answer 1

• provide presumptive diagnosis
• enchanced quality of patient care through rapid results and cost-effectiveness
• plays a significant role in quality control

Question 2

Observed in media immediately surrounding or underneath the colony

Answer 2

Hemolysis

Question 3

Hemolysis

Most important in presumptive identification of?

Answer 3

Streptococcus

Question 4

Hemolysis

Uses what light source?

Answer 4

Transillumination (light source behind the plate)

Question 5

Hemolysis

Two major types

Answer 5

a-hemolysis
B-hemolysis

Question 6

Hemolysis

Partial clearing of blood, green discoloration

Answer 6

a-hemolysis

Question 7

Hemolysis

Examples of a-hemolytic bacteria

Answer 7

Streptococcus pneumoniae, viridans strep

Question 8

Hemolysis

Complete clearing of blood cells

Answer 8

B hemolysis

Question 9

Hemolysis

Examples of B hemolysis

Answer 9

• S. pyogenes
• S. agalactiae
• Listeria monocytogenes

Question 10

Hemolysis

Non hemolytic colonies are referred to as?

Answer 10

y hemolytic

Question 11

Colony size

Answer 11

• Large
• Medium
• Small
• Pinpoint

Question 12

This helps suggest what you might find on Gram stain

Answer 12

Colony Size

Question 13

Colony Size

Gram-positive is ____ than gram-negative

Answer 13

less

Question 14

Colony Size

Staphylococcus is _____ than Streptococcus

Answer 14

greater

Question 15

Edge of colonies

Answer 15

• Smooth
• Filamentous
• Rough
• Rhizoid

Question 16

This bacteria has filamentous edge of colonies.

Answer 16

Bacillus anthracis

Question 17

These bacterial colonies have rough edges.

Answer 17

Diphtheroid

Question 18

Form or Margin

This is referred to as a hazy blanket of growth on surface.

Answer 18

Swarming

Question 19

Form or Margin

Examples of bacteria with Swarming colonies

Answer 19

Proteus spp.

Question 20

Description of a colony in which light can pass through

Answer 20

Transluscent colony

Question 21

T/F: There is no such thing as colorless or transparent colony.

Answer 21

True

Question 22

Elevation

Answer 22

• Raised
• Convex
• Flat
• Umbilicate
• Umbonate

Question 23

Elevation

Raised flat top

Answer 23

Raised

Question 24

Elevation

Dome shaped

Answer 24

Convex

Question 25

Elevation Not raised

Answer 25

Flat

Question 26

Elevation Convex with depressed center (pitting)

Answer 26

Umbilicate

Question 27

Elevation Examples of Umbilicate

Answer 27

S. pneumoniae (if no capsule)

Question 28

Elevation Convex with protruding nipple

Answer 28

Umbonate

Question 29

Elevation Examples of Umbonate

Answer 29

Diphtheroids

Question 30

Density

Answer 30

- Transparent - Transluscent - Opaque

Question 31

Semiopaque (bull's eye colony)

Answer 31

Group B strep (mostly Staph and Gr (-) rods)

Question 32

Color Coagulase negative staph

Answer 32

White

Question 33

Color Enterococcus and most gram-negative rods

Answer 33

gray

Question 34

Color Micrococcus and Neisseria

Answer 34

yellow or off-white

Question 35

Color Diphtheroids

Answer 35

buff

Question 36

This is determined by touching a colony with a loop.

Answer 36

Consistency

Question 37

Consistency

Answer 37

- Brittle (splinters) - Creamy - Dry - Waxy - Sticky

Question 38

Consistency Entire colony comes off the plate

Answer 38

Sticky

Question 39

Consistency Creamy

Answer 39

Staphyloccocus aureus

Question 40

Consistency Sticky

Answer 40

Neisseria

Question 41

Consistency Brittle

Answer 41

Nocardia

Question 42

Consistency Dry

Answer 42

Streptococci

Question 43

Consistency Dry and waxy

Answer 43

Diphtheroid

Question 44

Pigment Green or green metallic sheen

Answer 44

Pseudomonas aeruginosa

Question 45

Pigment Brick red

Answer 45

Serratia marcescens

Question 46

Pigment Blue

Answer 46

Kluyvera

Question 47

Pigment Purple

Answer 47

Chromobacterium violaceum

Question 48

Pigment Brown-black anaerobe

Answer 48

Prevotella melaninogenica

Question 49

Odor Old sock

Answer 49

S. aureus

Question 50

Odor Fruity or grape-like

Answer 50

P. aeruginosa

Question 51

Odor Putrid

Answer 51

Proteus mirabilis

Question 52

Odor Musty basement

Answer 52

Haemophilus spp.

Question 53

Odor Freshly plowed field

Answer 53

Nocardia spp.

Question 54

Organisms in Liquid Media Streamers

Answer 54

- Vinelike growth - Scumlike growth

Question 55

Organisms in Liquid Media Vinelike growth, puff ball-like

Answer 55

Streptococci

Question 56

Organisms in Liquid Media Scumlike growth

Answer 56

Yeast

Question 57

Organisms in Liquid Media Refers to overall cloudiness of liquid

Answer 57

Turbidity

Question 58

Organisms in Liquid Media Gas bubbles present

Answer 58

Enterics

Question 59

Oxidative processes require?

Answer 59

oxygen

Question 60

What medium is used in Carbohydrate oxidation and fermentation?

Answer 60

Oxidative-fermentative medium

Question 61

This determines the ability of bacteria to produce enzymes

Answer 61

Amino acid degradation

Question 62

What medium is used for amino acid degradation?

Answer 62

Lysine Iron Agar (LIA)

Question 63

Enzymes in amino acid degradation can:

Answer 63

- Deaminate - Dihydrolyze - Decarboxylate

Question 64

Level 1: Critical/invasive

Answer 64

- Amniotic fluid - blood - brain - CSF - heart valve - pericardial fluid

Question 65

Level 2: Unpreserved

Answer 65

- Bloody fluids - Bone - wound drainage - feces - sputum - tissue

Question 66

Level 3: Quantitation Required

Answer 66

- Catheter tip - Urine - Tissue for quantification

Question 67

Level 4: Preserved

Answer 67

- Feces in preservative - Urine in preservative - Swabs in holding medium

Question 68

Traditional Biochemical Testing Panel

Answer 68

- Citrate - TSI - LIO - MIO/SIM - Urease - Glucose - Lactose - Maltose