Week 21 Flashcards
(23 cards)
What is histology?
The microscopic study of tissues of the body and how these tissues are arranged to constitute an organ.
What are the three main steps in tissue preparation?
- Fixation
- Sectioning
- Staining
What is the purpose of fixation in tissue preparation?
To preserve the tissues and inactivate all biologically active materials.
What is sectioning in histology?
Slicing the tissues to observe a monolayer under the microscope.
What is the gold standard staining method in histology?
Haematoxylin and Eosin (H&E) staining.
What does Haematoxylin stain?
The nuclei blue.
What does Eosin stain?
The cytoplasm pink.
What is histopathology?
The microscopic study of diseased tissue.
Why is histopathology important in biomedical research?
It is crucial for the accurate diagnosis of cancer and other diseases.
What are the components analyzed in a blood smear?
- RBCs
- WBCs
- Platelets
What is the role of RBCs?
To carry oxygen around the body.
What is the role of WBCs?
Key players of the immune system.
What is the role of platelets?
Important for blood clotting.
Name a blood disorder that can be diagnosed using blood smears.
Iron-deficiency anaemia.
What type of cells are neutrophils?
They have a multilobed nucleus with light pinkish granules.
What is the appearance of eosinophils?
Very rare cells containing bright reddish granules.
What characterizes basophils?
They contain dark purple granules and are very rare cells.
Describe monocytes.
Large cells with a bean-shaped nucleus, filling at least ½ of the cytoplasm.
What distinguishes lymphocytes?
Small cells with a round nucleus that fills most of the cytoplasm.
What is the significance of identifying WBCs?
It helps diagnose various blood-related disorders.
Fill in the blank: The microscopic study of diseased tissue is called _______.
Histopathology
True or False: Eosin stains the nuclei blue.
False
What should be the normal value for a WBC count?
Typically between 4,000 and 11,000 cells per microliter.
