Intro

Question 1

Analyte

Answer 1

Substance whose chemical constituents are being identified

Question 2

Serum Sample

Answer 2

The fluid component of blood that is harvested after centrifugation of a clotted sample

Question 3

EDTA

Answer 3

Purple top. For routine CBC

Question 4

Citrate

Answer 4

Blue top. For coagulation testing. Bond to Ca and override anticoagulant effect

Question 5

Heparin

Answer 5

Green top. Blood gas analysis and many others. Antithrombin and form ionic bond with Ca

Question 6

Hematology assays

Answer 6

Completed on whole anticoagulated blood
(CBC, Coag)

Question 7

Clinical chemistry assays

Answer 7

Completed on serum, plasma, urine and other fluid
Detect concentration of analyte
Can be quantitative, semiquantitative, or qualitative

Question 8

Reference interval

Answer 8

95% of the healthy population; 5% of the healthy animals will fall outside of the reference interval. Do this to maximize disease detection.

Question 9

Analytical accuracy

Answer 9

Ability of assay to get agreement between measured value and the true value

Question 10

Analytic precision

Answer 10

Ability of a test to get the same result if a sample is analyzed several times

Question 11

Analytic specificity

Answer 11

Ability to identify only the analyte of interest

Question 12

Analytical sensitivity

Answer 12

How much change in concentration is required for assay to see the change

Question 13

Detection limit

Answer 13

Lowest concentration of a substance that can be accurately detected by the assay

Question 14

Most common type of laboratory errors

Answer 14

Pre-analytic (collection, handling, or labeling)