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Bacteriology > Campylobacter and Helicobacter > Flashcards

Campylobacter and Helicobacter Flashcards

1
Q

What is the habitat of campylobacter?

A

intestinal and genital tracts of domestic animals

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2
Q

General appearance of campylobacter

A

slender, curved, gram-neg rods in gull-wing shapes and spiral forms

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3
Q

Oxygen requirements of campylobacter

A

microaerophilic

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4
Q

Culture requirements of campylobacter

A

MAC or enriched media - skirrow campylobacter agar, butxler agar, charcoal agar
note: motile, non-fermentative, oxidase-pos

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5
Q

What temperature does C. jejuni grow at?

A

thermophilic - 42C

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6
Q

What temperature does C. fetus grow at?

A

25C

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7
Q

Diagnosis of disease causeed by campylobacter

A

culture and ID
staining with dilute carbol fuchsin
PCR

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8
Q

Virulence factors of campylobacter

A

LPS
microcapsule (S layer)
outer membrane proteins
adhesion proteins
flagella
type IV secretion system

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9
Q

Treatment and control of campylobacter

A

antibiotics - depends on strain and species effected
isolation of sick
vaccination

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10
Q

What antibiotics are campylobacter spp resistant to?

A

quinolones

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11
Q

Causative agent of Bovine Genital Campylobacteriosis

A

Campylobacter fetus subsp venerealis

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12
Q

Transmission of campylobacter fetus subsp venerealis

A

during coitus
bulls = indefinite carriers
1/3 cows = carriers

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13
Q

Diagnosis of Bovine Genital Campylobacteriosis

A

isolation and identificatio (does require special transport media)
FA
vaginal mucus agglutination test
ELISA
PCR

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14
Q

Treatment of Bovine Genital Campylobacteriosis

A

dihydrostreptomycin - systemic, topically, intrauterine

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15
Q

Is there a vaccination to prevent Bovine Genital Campylobacteriosis?

A

yes

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16
Q

Causative agent of Ovine Genital Campylobacteriosis

A

campylobacter fetus subsp fetus
campylobacter jejuni

17
Q

How is ovine genital campylobacteriosis trasnmitted?

A

fecal-oral route

18
Q

Clinical signs of ovine genital campylobacteriosis

A

necrotic pplacentitis - late abortions
stillborn or weak lambs
in aborted lambs: round necrotic lesions with pale raised rims and dark depressed centers on liver surface

19
Q

Is there any immunity associated with natural infection (and recovery) from ovine genital campylobacteriosis?

A

yes
recovered ewes are immune for at least 3 years

20
Q

Diagnosis of ovine genital campylobacteriosis

A

hepatic lesions in aborted lambs
culture and isolation

21
Q

Treatment and control of ovine genital campylobacteriosis

A

isolate aborting ewes
remove aborted fetuses and placentae
vaccination
chlortetracycline

22
Q

Causative agent of avian vibronic hepatitis

A

campylobacter jejuni

23
Q

Transmission of avian vibronic hepatitis

A

chicks aquire infection from feed, water and litter

24
Q

Clinical signs of avian vibronic hepatitis

A

usually asymptomatic
drop in egg production
listless
loss of condition
hemorrhage and multifocal liver necrosis

25
Q

Is avian vibronic hepatitis zoonotic?

A

yes

26
Q

Diagnosis of avian vibronic hepatitis

A

demonstration of curved rods with darting motility in bile using ohase contrast microscopy

27
Q

Treatment of avian vibronic hepatitis

A

dihydrostreptomycin in foos - early

28
Q

Causative agent of intestinal campylobacteriosis in dogs

A

campylobacter spp

29
Q

Which group of dogs are most at risk for intestinal campylobacteriosis?

A

immunosuppressed dogs - shed in feces of healthy dogs (usually self limiting)

30
Q

Diagnosis of intestinal campylobacteriosis in dogs

A

large number of campylobacter-like organisms in DCF-stained fecal smear or rectal scrapings

31
Q

Treatment of intestinal campylobacteriosis in dogs

A

erythromycin
but usually self-limiting

32
Q

General characteristics of helicobacter spp

A

helical shaped
gram neg
microaerophilic
oxidase +
catalase +

33
Q

Habitat of helicobacter

A

GIT of healthy dogs and cats

34
Q

Disease conditions associated with non-helicobacter pylori helicobacters

A

usually asymptomatic
diarrhea
vomting
weight loss
emaciation

35
Q

Disease conditions associated with H. pyloris

A

mostly associated with human GIT infestions
found in dogs, cats and rhesus macaques

36
Q

Diagnosis of helicobacter

A

difficult to culture
PCR
gastric biopsy
urease test
histology

37
Q

Treatment of GI helicobacter infection in dog/cat

A

combination antibiotics + stomach acid inhibitor

Bacteriology (26 decks)

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