1.8.1 Fixation (BG)

Question 1

What is the primary goal for fixation?

Answer 1

preservation of tissues

Question 2

What is the Secondary goal for fixation?

Answer 2

Harden the tissue
Protect the tissues from trauma and damage from different reagents

Question 3

The purpose of hardening the tissues

Answer 3

for easy cutting

Question 4

What are the 2 general methods of fixation?

Answer 4

Physical and Chemical

Question 5

What type from 2 general methods of fixation:

Heat fixation
Microwave
Freeze drying (Cryopreservation)

Answer 5

Physical fixation

Question 6

In physical fixation, what is seldom used (to fix bacterial smear)?

Answer 6

Heat fixation

Question 7

What type from 2 general methods of fixation:

Involves chemical fixatives

Answer 7

Chemical fixation

Question 8

According to composition:

Simple fixative has only 1 component

T or F

Answer 8

T

Question 9

NOTE:

Fixation methods (Fixatives

a. According to composition
b. According to action
c. According to MOA
d. According to active component substance

Question 10

According to composition

Compound fixative has 2 or more fixative

T or F

Answer 10

T

Question 11

According to action:

1. Microanatomical
2. Cytological (Nuclear, Cytoplasmic)
3. Histochemical

T or F

Answer 11

T

Question 12

According to MOA:

a. Additive
b. Non-additive

T or F

Answer 12

T

Question 13

According to MOA:

Binds to the tissue to preserve

a. Additive
b. Non-additive

Answer 13

a

Question 14

According to MOA:

Do not Binds to the tissue to preserve

a. Additive
b. Non-additive

Answer 14

b

Question 15

Non-Additives examples?

Answer 15

Alcohol and acetones

Question 16

According to active component substance:

1. Aldehyde (Formaldehyde, Glutaraldehyde)
2. Picric acid Fixatives (Picric acid: Bouin’s, Brasil’s, Hollandes)
3. Alcohol fixatives (Ethanol, Methanol, etc.)