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MTAP Histo > 1.12.1 Infiltration and Embedding > Flashcards

1.12.1 Infiltration and Embedding Flashcards

1
Q

Remove clearing agent and to fill up cavities of tissue space

A

Impregnation / Infiltration

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2
Q

Most common reagent for Infiltration?

A

Paraffin wax

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3
Q

Manual = Oven

Automatic = Autotechnicon

Vacuum

T or F

A

T

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4
Q

2 methods for cellodin infiltration:

Wet
Dry

Gelatin infiltration method = For studies

T or f

A

T

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5
Q

Paraffin wax infiltration:

Melting point commonly used?

A

56 C

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6
Q

Paraffin wax infiltration:

Not suited for?

A

Fatty tissues

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7
Q

Paraffin wax infiltration:

Manual method requires __________ changes of Wax

A

4

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8
Q

Paraffin wax infiltration:

Automatic method requires __________ changes of wax

A

2-3

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9
Q

Substitute for paraffin wax recommendation for bones and brain specimen

A

Paraplast

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10
Q

Paraffin wax is a Rapid reagent due to heat

T or F

A

T

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11
Q

Substitute for paraffin wax that is water soluble and is recommended for enzyme histochemical studies

A

Carbowax

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12
Q

Temperature range of paraffin oven when in used

A

55-60C

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13
Q

Approximate temperature of paraffin Oven when in used

A

2-5 deg C higher than the wax melting point

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14
Q

Infiltration under negative atmospheric pressure inside the oven

A

Vacuum infiltration

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15
Q

Substitute for paraffin wax used for eye specimens

A

Bioloid

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16
Q

Substitute for paraffin wax that will require heavy duty type of microtome, harder than P. wax

A

Esterwax

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17
Q

Melting point of paraplast

A

56 - 57

18
Q

Melting point of Ester wax

A

46-48

19
Q

Celloidin infiltration:

Disadvantage?

A

Slow due to no heat

20
Q

Celloidin infiltration:
Methods:
____________ for eyes. Uses GILSON’s mixture to store blocks

A

Dry

21
Q

Celloidin infiltration:
Methods:
____________ for Bones, brain, and whole organ

A

Wet

22
Q

Celloidin infiltration:

Wet Method uses ______________ tp store the block prior to sectioning

A

70-80% alcohol

23
Q

Infiltration method for enzyme and histochemical studies

A

Carbowax

24
Q

Celloidin infiltration:
Methods:

Composition of Gilson’s mixture? use to store prior to sectioning

A

Chloroform and Cedarwood oil

25
Q

Infiltration method that will require tissues not to be more than 2-3 mm thick

A

Gelatin infiltration

26
Q

A disposable embedding mold that will require smearing of the inner mold with glycerine

A

Plastic ice trays

27
Q

An embedding mold consisting of a series of interlocking plates resting on a flat metal base

A

Compound embedding unit

28
Q

Disposable mold that gives perfect blocks without trimming

A

Peel-away mold

29
Q

COmpound embedding unit advantage:

Can embed more than 1 tissues

T or F

A

T

30
Q

Other term for embedding:

Blocking
Casting

T or F

A

T

31
Q

Process of infiltrating tissu with celloidin and embedding with paraffin

A

Double embedding

32
Q

Embedding mold consisting of L shaped strips made of heavy brass

A

Leukchart’s

33
Q

Use of Phenol in gelatin infiltration

A

To prevent growth of mold

34
Q

Celloidin infiltration:

Thin: ____
Medium :____
Thick:_____

A

2-4%
4-6%
8-12%

35
Q

Plastic resins include:

Spurs
Polyester
Polyglycl methacrylate / GMA
Methylmethcrylate / MMA

T or F

A

T

36
Q

Fastest Epoxy resin?

A

Spurr

37
Q

Plastic resin originally introduced for EM

A

Polyester

38
Q

Extremely hydrophilic

Polyglycl methacrylate / GMA
Methylmethcrylate / MMA

A

Polyglycl methacrylate / GMA

39
Q

Plastic resin considered ideal for undecalcified bones and hard tissues

Polyglycl methacrylate / GMA
Methylmethcrylate / MMA

A

Methylmethcrylate / MMA

40
Q

what is the product of embedding?

A

Tissue block

MTAP Histo (20 decks)

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