1.12.1 Infiltration and Embedding

Question 1

Remove clearing agent and to fill up cavities of tissue space

Answer 1

Impregnation / Infiltration

Question 2

Most common reagent for Infiltration?

Answer 2

Paraffin wax

Question 3

Manual = Oven

Automatic = Autotechnicon

Vacuum

T or F

Answer 3

T

Question 4

2 methods for cellodin infiltration:

Wet
Dry

Gelatin infiltration method = For studies

T or f

Answer 4

T

Question 5

Paraffin wax infiltration:

Melting point commonly used?

Answer 5

56 C

Question 6

Paraffin wax infiltration:

Not suited for?

Answer 6

Fatty tissues

Question 7

Paraffin wax infiltration:

Manual method requires __________ changes of Wax

Answer 7

4

Question 8

Paraffin wax infiltration:

Automatic method requires __________ changes of wax

Answer 8

2-3

Question 9

Substitute for paraffin wax recommendation for bones and brain specimen

Answer 9

Paraplast

Question 10

Paraffin wax is a Rapid reagent due to heat

T or F

Answer 10

T

Question 11

Substitute for paraffin wax that is water soluble and is recommended for enzyme histochemical studies

Answer 11

Carbowax

Question 12

Temperature range of paraffin oven when in used

Answer 12

55-60C

Question 13

Approximate temperature of paraffin Oven when in used

Answer 13

2-5 deg C higher than the wax melting point

Question 14

Infiltration under negative atmospheric pressure inside the oven

Answer 14

Vacuum infiltration

Question 15

Substitute for paraffin wax used for eye specimens

Answer 15

Bioloid

Question 16

Substitute for paraffin wax that will require heavy duty type of microtome, harder than P. wax

Answer 16

Esterwax

Question 17

Melting point of paraplast

Answer 17

56 - 57

Question 18

Melting point of Ester wax

Answer 18

46-48

Question 19

Celloidin infiltration:

Disadvantage?

Answer 19

Slow due to no heat

Question 20

Celloidin infiltration:
Methods:
____________ for eyes. Uses GILSON’s mixture to store blocks

Answer 20

Dry

Question 21

Celloidin infiltration:
Methods:
____________ for Bones, brain, and whole organ

Answer 21

Wet

Question 22

Celloidin infiltration:

Wet Method uses ______________ tp store the block prior to sectioning

Answer 22

70-80% alcohol

Question 23

Infiltration method for enzyme and histochemical studies

Answer 23

Carbowax

Question 24

Celloidin infiltration:
Methods:

Composition of Gilson’s mixture? use to store prior to sectioning

Answer 24

Chloroform and Cedarwood oil

Question 25

Infiltration method that will require tissues not to be more than 2-3 mm thick

Answer 25

Gelatin infiltration

Question 26

A disposable embedding mold that will require smearing of the inner mold with glycerine

Answer 26

Plastic ice trays

Question 27

An embedding mold consisting of a series of interlocking plates resting on a flat metal base

Answer 27

Compound embedding unit

Question 28

Disposable mold that gives perfect blocks without trimming

Answer 28

Peel-away mold

Question 29

COmpound embedding unit advantage: Can embed more than 1 tissues T or F

Answer 29

T

Question 30

Other term for embedding: Blocking Casting T or F

Answer 30

T

Question 31

Process of infiltrating tissu with celloidin and embedding with paraffin

Answer 31

Double embedding

Question 32

Embedding mold consisting of L shaped strips made of heavy brass

Answer 32

Leukchart's

Question 33

Use of Phenol in gelatin infiltration

Answer 33

To prevent growth of mold

Question 34

Celloidin infiltration: Thin: ____ Medium :____ Thick:_____

Answer 34

2-4% 4-6% 8-12%

Question 35

Plastic resins include: Spurs Polyester Polyglycl methacrylate / GMA Methylmethcrylate / MMA T or F

Answer 35

T

Question 36

Fastest Epoxy resin?

Answer 36

Spurr

Question 37

Plastic resin originally introduced for EM

Answer 37

Polyester

Question 38

Extremely hydrophilic Polyglycl methacrylate / GMA Methylmethcrylate / MMA

Answer 38

Polyglycl methacrylate / GMA

Question 39

Plastic resin considered ideal for undecalcified bones and hard tissues Polyglycl methacrylate / GMA Methylmethcrylate / MMA

Answer 39

Methylmethcrylate / MMA

Question 40

what is the product of embedding?

Answer 40

Tissue block