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MTAP Histo > 1.9.2 Decalcification (In Notes) > Flashcards

1.9.2 Decalcification (In Notes) Flashcards

1
Q

Potent anticoagulant but weak decalcifying agent

A

EDTA

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2
Q

Binds with calcium to form weakly dissociated complexes

A

EDTA

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3
Q

Decalcification method that involves attraction of positively charged calcium ions with negatively charged electrodes

A

Electrolytic method

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4
Q

Decalcifying acid used for surface decalcification of blocks

A

HCl (Von Ebner’s: HCl + NSS)

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5
Q

Both decalcifying and a tissue softener

A

Perenyi’s fluid

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6
Q

Phenol
Molliflex
Perenyis

These are known as?

A

Tissue softeners

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7
Q

Most commonly used decalcifying agent especially recommended for urgent biopsis for needle and small biopsies

A

5-10% HNO3

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8
Q

Temp range for decal?

A

18-30C

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9
Q

Ratio of decal agents ti tissue vol?

A

20:1

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10
Q

Most reliable method of testing for the completeness of decalcification

A

X-Ray

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11
Q

Method of testing for the completeness of decalcification that uses calcium carbonate

A

Bubble test

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12
Q

Presence of bubbles in bubbles test = Decal is not yet complete

T or F

A

T

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13
Q

Duration of decalcification thur ion exchange resin

A

14 days

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14
Q

Method of testing for the completeness of decalcification done by probing tissue with a needle

A

Physical / Mechanical

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15
Q

Most rapid nitric-containing decalcifying

A

Phloroglucin-Nitric

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16
Q

An excellent bone decalcifier for EM and enzyme histochemistry

A

EDTA

17
Q

Remedy for inactivation of alkaline phosphatase by EDTA

A

Magnesium chloride

18
Q

Acid > Nitirc > Dilute:

5-10% HNO3
Formol Nitric
Perenyi’s
Pholoroglucin-Nitric

These are used Instead of NITRIC form

T or F

A

T

19
Q

Pure Nitric form is not used in decalcification

T or F

A

T

20
Q

Factors affecting decalcification:

  1. Degree of mineralization
  2. Concentration of dec. agent
  3. Temp

T or F

A

T

MTAP Histo (20 decks)

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