2.2.2 Staining Methods (SEM)

Question 1

Uses aqueous or alcoholic solutions of dye

Answer 1

Direct staining

Question 2

Staining technique that employs use of mordant and accentuator

Answer 2

Indirect staining

Question 3

Indirect staining:

A substance that serves as link between the dye and the tissue? Ex?

Answer 3

Mordant:

Potassium alum
Iron

Question 4

Indirect staining:

Substance that increases staining power of the dye

It heightens color intensity of the dye

ex?

Answer 4

Accentuator:

KOH
Phenol

Question 5

Tx + dye = color

Answer 5

Direct staining

Question 6

Tx + dye + M or A = color

Answer 6

Indirect staining

Question 7

Mayers hematoxylin, Ehrlich;s hematoxylin, Harris Hematoxylin

These are an example of?

Answer 7

Forms of Hematoxylin with K-ALUM as MORDANT

ALUM hematoxylin

Question 8

Weigert’s hematoxylin, Heidenhain’s hematoxylin

Answer 8

Forms of hematoxylin with IRON as MORDANT

IRON hematoxylin

Question 9

Involves gradual application of dye

Follows definite sequence

No excess dye
No decolorization needed

Answer 9

Progressive staining

Question 10

Regressive staining involves

Overstaining the tissue
There is Excess dye
We do decolorization

T or F

Answer 10

T

Question 11

Selective removal of excess dye

Answer 11

Decolorization / Differentiation

Question 12

Decolorization / Differentiation most commonly used?

Answer 12

Acid alcohol

Question 13

Staining technique that involves use of dye/stain that gives a different color, different from the color of the dye itself

Answer 13

Metachromatic staining

Question 14

Mainly used for demonstration of cartilage CT, epithelial mucins, mast cells granule, amyloid

Answer 14

Metachromatic staining

Question 15

Methyl Violet
Crystal violet
Safranin
Bismarck Brown
Basic fuchsin
Methylene blue
Thionine
Toluidine Blue
Azure A, B, C
Cresylblue

These are all examples of?

Answer 15

Metachromatic staining

Question 16

Staining technique in which tissue components are stained with the same shade or hue as that of the dye?

Answer 16

Orthochromasia

Question 17

Staining method that involves the use of another dye to provide contrast and background

Answer 17

Counter staining

Question 18

Counter staining:

Most Commonly used?

Answer 18

Eosin

Question 19

Tissue components are demonstrated not by stains but by colorless solutions of metallic salts that produces black deposits on the surface of tissues

Answer 19

Metallic impregnation

Question 20

Examples of agents used in metallic impregnation?

Answer 20

Gold chloride

Silver nitrate

Extra:
Ammonia cal Silver

Question 21

Staining of living cell components involves cytoplasmic phagocytosis

Answer 21

Vital staining

Question 22

Methods of Vital staining

Answer 22

Intravital
Supravital

Question 23

Injecting the dye to any part of the living body

Answer 23

Intravital staining

Question 24

Involves immediate application of stain to living cells following removal from living body

Answer 24

Supravital staining

Question 25

The best vital dye

Answer 25

Neutral red (Supra)

Question 26

Supravital stain recommended for mitochrondia

Answer 26

Janus green

Question 27

Neutral red, Janus green, trypan blue, Nile blue, thionine, toluidine blue

These dyes belong to what stains?

Answer 27

Supravital stain