1.8.3 Fixative Con.

Question 1

For brain tissues for the diagnosis of rabies

Answer 1

Acetone, carnoy’s

Question 2

Excellent for Glycogen Demonstration

Answer 2

Picric Acid Fixatives

Question 3

For Embryos, pituitary biopsies and Endometrial curetting’s NOT for kidney structures

NOT COMPATIBLE with Feulgen’s

Answer 3

Bouin’s fluid

Question 4

For Touch prep

Answer 4

95% Isopropyl alcohol

Question 5

Solidifies at 17 C

Answer 5

Glacial HAC

Question 6

Must be used at ice cold temp

FOR enzymes

FIXATIVE and DEHYDRATING agent

Answer 6

Acetone

Question 7

For mucopolysaccharides and nuclear protein

Answer 7

Newcomer’s (Alcohol fixatives)

Question 8

Most rapid tissue fixative for fixing chromosomes and Lymph glands

Answer 8

Carnoy’s (Alc. fixative)

Question 9

Osmium Tetroxide, Glutaraldehyde, and Paraformaldehyde are use for what microscope?

Answer 9

Electron micrscope

Question 10

Fixation method that involves thrmal coagulation of proteins

Answer 10

Heat fixation

Question 11

Fixation folume for museum preparation should not be LESS THAN ____________

Answer 11

50-100x vol of Specimen

Question 12

Fixatives for enzyme histochemistry:

4% formaldehyde
Formol saline

Which of the following?

Answer 12

All

Question 13

Smallest aldehyde

Answer 13

Glyoxal

Question 14

Glyoxal is a rapid aldehyde fixative

t or f

Answer 14

T

Question 15

Main ingredient for Bouin’s Brasil and Hollande’s

Answer 15

Picric acid fixative

Question 16

Can be used for myelin and pripheral nerves

Answer 16

Osmium tetroxide

Question 17

Osmium tetroxide is not usually for routine fixative due to ________ acting fixative, expensive and req 5-10x vol of the specimen

Answer 17

Slow

Question 18

Proposed as mercuric chloride substitute

Answer 18

ZnSO4 (Zinc sulfate)

Question 19

Can be used as fixative, decalcifying agent and stain

Answer 19

Picric acid

Question 20

Often used in conjunction with other fixatives to produce compound fixative

Answer 20

Glacial Acetic acid (HAc)

Question 21

Alcohol and aldehyde is use for dehydration and fixation

T or F

Answer 21

T

Question 22

Picric acid stain imparts what color?

Answer 22

Yellow