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MTAP Histo > 2.1.1 Sectioning > Flashcards

2.1.1 Sectioning Flashcards

1
Q

Type of microtome used for paraffin-embedded tissues

A

Rotary microtome

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2
Q

Rotary microtome Invented by?

A

Minot

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3
Q

Microtome type for cutting celloidin embedded tissues

A

Sliding

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4
Q

2 types of sliding:

Base
Sledge

T or F

A

T

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5
Q

Sliding microtome is Develop by?

A

Adam’s

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6
Q

Microtome type for EM

A

Ultrathin microtome

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7
Q

Thickness of sections in Ultrathin microtome

A

0.5 u

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8
Q

Types of Microtomes for preparing frozen sections:

A

Freezing microtome
Cryostat / Cold microtome

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9
Q

Purpose of frozen sections

A

Rapid diagnosis

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10
Q

Thickness of sections using rotary microtome?

A

4-6 u

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11
Q

Refrigerated apparatus for preparing frozen sections

A

Cryostat

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12
Q

Temp for cryostat

A

-5 to - 30C

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13
Q

Liquid nitrogen, isopentane cooled by liquid nitrogen, CO2 gas, and aerosol sprays

A

Freezing agents

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14
Q

Frozen section is used for Rapid Diagnosis while patient is in the OR (FRESH tissues)

Which is why Cryostat is use seems in needs Immediately

T or F

A

T

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15
Q

Regarded as the most dangerous type of microtome

A

Sliding microtome

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16
Q

Most common microtome

A

Rotary microtome

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17
Q

Purpose of Float out bath?

A

Prevents wrinkles

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18
Q

Commonly used tissue adhesive

A

Mayer’s egg albumin

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19
Q

Mayer’s egg albumin is Prepared by

A

Egg white + glycerine

20
Q

Adding thymol crystal in tissue adhesive to prevent growth of?

A

Mold

21
Q

Types of Microtome knives

A

Plane concave
Plane wedge
Biconcave

22
Q

Microtome knives is not routinely used since we use?

A

Disposable knives

23
Q

Biconcave is used for?

A

Cut paraffin-embedded tissues

24
Q

Plane wedge knife can be used for

A

Cut frozen sections
Cut extremely hard and tough tissues

25
Q

Biconcave: Both sides are concave
Plane wedge: Both sides are flat
Plane concave: 1 side flat, 1 side concave

T or F

A

t

26
Q

Plane concave:

Concave part:
Flat:

A

For paraffin-embedded

For celloidin-embedded tissues

27
Q

Grinding the cutting edge to form even edge

A

Honing

28
Q

Honing Purposes?

A

Remove gross nicks

29
Q

Types of Hones (Oil stones):
Belgium yellow:___________

A

Gives best results

30
Q

Types of Hones (Oil stones):

More polishing effect than Belgium yellow

A

Arkansas

31
Q

Types of Hones (Oil stones):

For badly nicked knives

A

Fine Carborundum

32
Q

Mineral oil, Clove oil, xylene, liquid paraffin and soapy water

These are an example of?

A

Lubricants for honing

33
Q

Process of removing burrs and other irregularities formed during honing

To polish or sharpen the cutting edge

A

Stropping

34
Q

Material used for Stropping:

A

Horse leather (Must be oiled prior to use)

35
Q

Angle formed between the cutting facet of the knife and the surface of the block

A

Clearance angle / Inclination angle / Tilt angle

36
Q

Clearance angle / Inclination angle / Tilt angle is set at what angle?

A

5-10 degrees

37
Q

Angle formed by the sides of the knife

A

Wedge angle

38
Q

Wedge angle is set at what angle?

A

14-15 degrees

39
Q

Angle formed between cutting edges

A

Bevel angle

40
Q

Bevel angle is set at?

A

27-32 degrees

41
Q

This flattens the ribbons and remove wrinkles and folds

A

Float out bath

42
Q

Float out bath temp?

A

45-50

43
Q

Float out bath:

5-10 deg lower than the melting point

T or F

A

T

44
Q

Removal of paraffin wax from tissues once properly fixed on the slide

A

Deparaffinization

45
Q

Deparaffinization methods include

  1. Immersion of slides in xylene
  2. Place slides in the oven
  3. Use of alcohol lamp

T or F

A

T

MTAP Histo (20 decks)

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