2.1.1 Sectioning

Question 1

Type of microtome used for paraffin-embedded tissues

Answer 1

Rotary microtome

Question 2

Rotary microtome Invented by?

Answer 2

Minot

Question 3

Microtome type for cutting celloidin embedded tissues

Answer 3

Sliding

Question 4

2 types of sliding:

Base
Sledge

T or F

Answer 4

T

Question 5

Sliding microtome is Develop by?

Answer 5

Adam’s

Question 6

Microtome type for EM

Answer 6

Ultrathin microtome

Question 7

Thickness of sections in Ultrathin microtome

Answer 7

0.5 u

Question 8

Types of Microtomes for preparing frozen sections:

Answer 8

Freezing microtome
Cryostat / Cold microtome

Question 9

Purpose of frozen sections

Answer 9

Rapid diagnosis

Question 10

Thickness of sections using rotary microtome?

Answer 10

4-6 u

Question 11

Refrigerated apparatus for preparing frozen sections

Answer 11

Cryostat

Question 12

Temp for cryostat

Answer 12

-5 to - 30C

Question 13

Liquid nitrogen, isopentane cooled by liquid nitrogen, CO2 gas, and aerosol sprays

Answer 13

Freezing agents

Question 14

Frozen section is used for Rapid Diagnosis while patient is in the OR (FRESH tissues)

Which is why Cryostat is use seems in needs Immediately

T or F

Answer 14

T

Question 15

Regarded as the most dangerous type of microtome

Answer 15

Sliding microtome

Question 16

Most common microtome

Answer 16

Rotary microtome

Question 17

Purpose of Float out bath?

Answer 17

Prevents wrinkles

Question 18

Commonly used tissue adhesive

Answer 18

Mayer’s egg albumin

Question 19

Mayer’s egg albumin is Prepared by

Answer 19

Egg white + glycerine

Question 20

Adding thymol crystal in tissue adhesive to prevent growth of?

Answer 20

Mold

Question 21

Types of Microtome knives

Answer 21

Plane concave
Plane wedge
Biconcave

Question 22

Microtome knives is not routinely used since we use?

Answer 22

Disposable knives

Question 23

Biconcave is used for?

Answer 23

Cut paraffin-embedded tissues

Question 24

Plane wedge knife can be used for

Answer 24

Cut frozen sections
Cut extremely hard and tough tissues

Question 25

Biconcave: Both sides are concave Plane wedge: Both sides are flat Plane concave: 1 side flat, 1 side concave T or F

Answer 25

t

Question 26

Plane concave: Concave part: Flat:

Answer 26

For paraffin-embedded For celloidin-embedded tissues

Question 27

Grinding the cutting edge to form even edge

Answer 27

Honing

Question 28

Honing Purposes?

Answer 28

Remove gross nicks

Question 29

Types of Hones (Oil stones): Belgium yellow:___________

Answer 29

Gives best results

Question 30

Types of Hones (Oil stones): More polishing effect than Belgium yellow

Answer 30

Arkansas

Question 31

Types of Hones (Oil stones): For badly nicked knives

Answer 31

Fine Carborundum

Question 32

Mineral oil, Clove oil, xylene, liquid paraffin and soapy water These are an example of?

Answer 32

Lubricants for honing

Question 33

Process of removing burrs and other irregularities formed during honing To polish or sharpen the cutting edge

Answer 33

Stropping

Question 34

Material used for Stropping:

Answer 34

Horse leather (Must be oiled prior to use)

Question 35

Angle formed between the cutting facet of the knife and the surface of the block

Answer 35

Clearance angle / Inclination angle / Tilt angle

Question 36

Clearance angle / Inclination angle / Tilt angle is set at what angle?

Answer 36

5-10 degrees

Question 37

Angle formed by the sides of the knife

Answer 37

Wedge angle

Question 38

Wedge angle is set at what angle?

Answer 38

14-15 degrees

Question 39

Angle formed between cutting edges

Answer 39

Bevel angle

Question 40

Bevel angle is set at?

Answer 40

27-32 degrees

Question 41

This flattens the ribbons and remove wrinkles and folds

Answer 41

Float out bath

Question 42

Float out bath temp?

Answer 42

45-50

Question 43

Float out bath: 5-10 deg lower than the melting point T or F

Answer 43

T

Question 44

Removal of paraffin wax from tissues once properly fixed on the slide

Answer 44

Deparaffinization

Question 45

Deparaffinization methods include 1. Immersion of slides in xylene 2. Place slides in the oven 3. Use of alcohol lamp T or F

Answer 45

T