Histology - Intro to Histology

Question 1

, focused with optincal lens (max resoving poswr ~0.2 microns).

Answer 1

Light microscopy (LM)

Question 2

Electrons interact with tissue components (B&W images). Focused with electromagnetic lens (resolution 1000x LM)

Answer 2

Electron microscopy (EM)

Question 3

Shows internal structure

Answer 3

Transmission (TEM)

Question 4

Shows external (surface) structure

Answer 4

Scanning (SEM)

Question 5

splits cell membranes (p-face, e-face)

Answer 5

TEM freeze fracture technique

Question 6

Interact with acids (basophilic structures). Stain acidic components blue or purple. E.g. nucleic acids (RNA< DNA, RER), glycosaminoclycans (GAGs)

Answer 6

Base-like stains

Question 7

Interact with bases (acidophilic structures). Stain basic components pink, red, or orange. E.g. mitochondria, collagen

Answer 7

Acid-like stains

Question 8

acid-like stain (reddish)

Answer 8

eosin

Question 9

base-like stain (blue)

Answer 9

hematoxylin

Question 10

Stains carbohydrates magenta. E.g., glycoproteins in mucin

Answer 10

Periodic-Acid Schiff (PAS)

Question 11

coffee bean staining pattern of nucleus

Answer 11

Brenner tumor

Question 12

Stains collagen fibers blue or green

Answer 12

Trichrome

Question 13

Stains reticular fibers black

Answer 13

Silver

Question 14

Stains elastic fibers reddish-brown or black

Answer 14

Orcein

Question 15

stains lipids red

Answer 15

Sudan Red

Question 16

stains lipids black

Answer 16

Osmic acid

Question 17

Look at nuclei (tend to be similar in size among cells)

Answer 17

How to see scale

Question 18

biological yardstick (~8 microns)

Answer 18

Red blood cell (RBS)

Question 19

tissue shrinkage & lost molecules, “cracks” & “spaces”

Answer 19

Artifacts