Flashcards in 25_Signal Transduction III Deck (12)
Where in a phospholipid does PLC cleave?
between the glycerol and the phosphate
What is the domain on PLC that interacts with a phosphorylated inositol ring?
(pleckstrin homology domain)
In the example of Ach-induced smooth muscle contraction, what triggers generation of phosphorylated lipids at the membrane?
activation of the GPCR
In the example of Ach-induced smooth muscle contraction, what phosphoinositide does PLC use as a substrate? What are the two 2nd messengers produced?
2nd messengers: IP3 and diacyl glycerol (DAG)
Where does IP3 bind?
IP3 receptors on the sarcoplasmic reticulum
Which ion's release is triggered by IP3?
What does DAG activate?
a protein kinase-C cascade
What are the second messengers involved in smooth muscle contraction?
IP3 & Ca2+
DAG (although exact function of DAG on cellular level is unclear)
Why is a wave of calcium observed in mammalian eggs upon fertilization?
IP3 is formed by a PLC brought into the egg by the sperm
What is an example of a compound used to visualize calcium in a living cell?
What second messengers are required for chemotaxis?