25_Signal Transduction III Flashcards Preview

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Flashcards in 25_Signal Transduction III Deck (12)
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1

Where in a phospholipid does PLC cleave?

between the glycerol and the phosphate

2

What is the domain on PLC that interacts with a phosphorylated inositol ring?

PH domain
(pleckstrin homology domain)

3

In the example of Ach-induced smooth muscle contraction, what triggers generation of phosphorylated lipids at the membrane?

activation of the GPCR

4

In the example of Ach-induced smooth muscle contraction, what phosphoinositide does PLC use as a substrate? What are the two 2nd messengers produced?

PI(4,5,)P2

2nd messengers: IP3 and diacyl glycerol (DAG)

5

Where does IP3 bind?

IP3 receptors on the sarcoplasmic reticulum

6

Which ion's release is triggered by IP3?

Ca2+

7

What does DAG activate?

a protein kinase-C cascade

8

What are the second messengers involved in smooth muscle contraction?

IP3 & Ca2+

DAG (although exact function of DAG on cellular level is unclear)

9

Why is a wave of calcium observed in mammalian eggs upon fertilization?

IP3 is formed by a PLC brought into the egg by the sperm

10

What is an example of a compound used to visualize calcium in a living cell?

Fura2

11

What second messengers are required for chemotaxis?

IPs

12

What is the effector protein in the IP3 production pathway?

PLC