Flashcards in 27_Steroid hormones & gene regulation Deck (15)
Which class of transcription factors have a ligand-binding domain?
nuclear hormone receptors
What is the function of the trans-activation domain on a TF?
recruitment of transcriptional partners/machinery
Which TF domain binds to DNA response elements?
Name some examples of steroid hormone receptors.
glucocorticoid receptor, estrogen receptor, thyroid hormone receptor, retinoic acid receptors
Where do nuclear hormone receptors bind their ligand?
in the cytoplasm
What tag is used to mark genes according to parental origin?
In DNA methylation, where is the methyl group added?
C5 position of cytosine residues
Where on the DNA does the GR bind?
GRE (glucocorticoid response element)
What is the chromatin remodelling complex recruited to acetylated histones? How does it affect chromatin?
It makes DNA accessible to RNA Pol/ other TF by either sliding the nucleosomes aside OR reorganizing them
Which TFs bind to the TATA box?
(overall, the TFIID complex... which includes TBP and TAFII250, as well as other TFs)
What does HDAC do?
HDAC = histone deacetylase
What is recruited to histones once deacetylated by HDAC?
Histone methyltransferases; K9 of H3 is methylated.
What are SMRT, N-CoR, and CoREST examples of?
What is Sin3 an example of?