27_Steroid hormones & gene regulation Flashcards Preview

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Flashcards in 27_Steroid hormones & gene regulation Deck (15)
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1

Which class of transcription factors have a ligand-binding domain?

nuclear hormone receptors

2

What is the function of the trans-activation domain on a TF?

recruitment of transcriptional partners/machinery

3

Which TF domain binds to DNA response elements?

DNA-binding domain

4

Name some examples of steroid hormone receptors.

glucocorticoid receptor, estrogen receptor, thyroid hormone receptor, retinoic acid receptors

5

Where do nuclear hormone receptors bind their ligand?

in the cytoplasm

6

What tag is used to mark genes according to parental origin?

DNA methylation

7

In DNA methylation, where is the methyl group added?

C5 position of cytosine residues

8

Where on the DNA does the GR bind?

GRE (glucocorticoid response element)

9

What is the chromatin remodelling complex recruited to acetylated histones? How does it affect chromatin?

SWI/SNF
It makes DNA accessible to RNA Pol/ other TF by either sliding the nucleosomes aside OR reorganizing them

10

Which TFs bind to the TATA box?

TBP

(overall, the TFIID complex... which includes TBP and TAFII250, as well as other TFs)

11

What does HDAC do?

HDAC = histone deacetylase

12

What is recruited to histones once deacetylated by HDAC?

Histone methyltransferases; K9 of H3 is methylated.

13

What are SMRT, N-CoR, and CoREST examples of?

transcriptional co-repressors

14

What is Sin3 an example of?

HDAC

15

What is SUV39H1 an example of?

Histone methyltransferase