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HPCT 311 > Adhesive and Staining (II) > Flashcards

Adhesive and Staining (II) Flashcards

(78 cards)

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1
Q

Most commonly used for histologic studies.

A

Hematoxylin

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2
Q

COMBINED WITH MORDANTS to form dye mordant-tissue complex.

A

Hematoxylin

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3
Q

FILTER stain prior to use.

A

Hematoxylin

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4
Q

Routinely used in H&E staining.

[hematoxylin]

A

Aluminum hematoxylin

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5
Q

Initially STAINS NUCLEI RED but after BLUEING forms blue lakes.

[hematoxylin]

A

Aluminum hematoxylin

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6
Q

For progressive and regressive staining.

[hematoxylin]

A

Aluminum hematoxylin

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7
Q

[6] Aluminum hematoxylin

[HED, MCC]

A
  1. Harris hematoxylin
  2. Ehrlich’s hematoxylin
  3. Delafield’s hematoxylin
  4. Mayer’s hematoxylin
  5. Cole’s hematoxylin
  6. Carazzi’s hematoxylin
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8
Q

Routinely used for nuclear staining.

[aluminum hematoxylin]

A

Harris hematoxylin

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9
Q

Ripened with mercuric chloride.

[aluminum hematoxylin]

A

Harris hematoxylin

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10
Q

CYTOLOGY: nuclear stain in PAP’s

[aluminum hematoxylin]

A

Harris hematoxylin

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11
Q

Staining of sex chromosomes.

[aluminum hematoxylin]

A

Harris hematoxylin

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12
Q

Staining method: Regressive

[aluminum hematoxylin]

A

Harris hematoxylin

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13
Q

Addition of GLACIAL ACETIC ACID: gives precise nuclear staining

[aluminum hematoxylin]

A

Harris hematoxylin

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14
Q

Excellent nuclear stain.

[aluminum hematoxylin]

A

Ehrlich’s hematoxylin

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15
Q

STAINS MUCIN, recommended for BONE and CARTILAGE.

[aluminum hematoxylin]

A

Ehrlich’s hematoxylin

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16
Q

Addition of GLYCERIN: slows oxidation and prolong shelf life.

[aluminum hematoxylin]

A

Ehrlich’s hematoxylin

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17
Q

Naturally ripened.

[aluminum hematoxylin]

A

Delafield’s hematoxylin

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18
Q

Chemically ripened with sodium iodate.

[aluminum hematoxylin]

A

Mayer’s hematoxylin

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19
Q

Artificially ripened with ALCOHOLIC IODINE.

[aluminum hematoxylin]

A

Cole’s hematoxylin

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20
Q

Artificially ripened with POTASSIUM IODATE.

[aluminum hematoxylin]

A

Carazzi’s hematoxylin

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21
Q

Uses IRON SALTS as both mordant and ripening agent.

[hematoxylin]

A

Iron hematoxylin

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22
Q

[4] Iron hematoxylin

[WHLV]

A
  1. Weigert’s hematoxylin
  2. Heidenhain’s hematoxylin
  3. Loyez hematoxylin
  4. Verhoeff hematoxylin
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23
Q

Mordant/oxidizer: Ferric ammonium chloride

[iron hematoxylin]

A

Weigert’s hematoxylin

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24
Q

Mordant/oxidizer of Weigert’s hematoxylin

A

Ferric ammonium chloride

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25
Standard iron hematoxylin. [iron hematoxylin]
Weigert's hematoxylin
26
Used in demonstrating muscle fibers and CT. [iron hematoxylin]
Weigert's hematoxylin
27
Mordant/oxidizer: Iron Alum [iron hematoxylin]
Heidenhainn's hematoxylin
28
CYTOLOGICAL STAIN: for nuclear and cytoplasmic inclusions. [iron hematoxylin]
Heidehainn's hematoxylin
29
Result: Gray-black [iron hematoxylin]
Heidenhainn's hematoxylin
30
Result color of Heidenhainn's hematoxylin
Gray-black
31
Mordant/oxidizer of Heidenhainn's hematoxylin.
Iron Alum
32
For frozen sections. [iron hematoxylin]
Loyez hematoxylin
33
Elastic fiber = black [iron hematoxylin]
Verhoeff hematoxylin
34
Mordant: 1% aqueous phosphotungstic acid. [hematoxylin]
Tungsten Hematoxylin
35
Oxidizer: potassium permanganate [hematoxylin]
Tungsten hematoxylin
36
For demonstration of ENDOCRINE CELLS of alimentary tract. [hematoxylin]
Lead hematoxylin
37
spermatogenesis [hematoxylin]
Copper hematoxylin
38
A red acid (xanthene) dye.
Eosin
39
[3] Stains connective tissue and cytoplasm differentially 3 forms.
1. Eosin Y 2. Eosin B, Erythrosin B 3. Eosin S, Ethyl Eosin
40
For demonstration of connective tissues (collagen). [other stains]
Acid Fuchsin – Picric Acid (Van gieson's stain)
41
Discriminates dead and living cells. [other stains]
Acridine orange
42
Demonstration of CALCIUM SALTS DEPOSITS and PHOPHATASE ACTIVITIES. [other stains]
Acridine red 3B
43
Water soluble phthalocyanine dye. [other stains]
Alcian blue
44
For connective tissue and epithelial mucin. [other stains]
Alcian blue
45
Acid mucopolysaccharide = blue [other stains]
Alcian blue
46
Cytoplasmic stain [other stains]
Aniline blue
47
For COUNTERSTAINING OF EPITHELIAL sections. [other stains]
Aniline blue
48
Plasma stain [other stain]
Basic fuchsin
49
For staining of acid fast organism, for mitochondria for differentiation of smooth muscles (with picric acid) [other stain]
Basic fuchsin
50
For staining hemoglobin [other stain]
Benzidine
51
Papanicolaou method (EA 50) [other stain]
Bismarck brown
52
Used for staining diphtheria organism. [other stain]
Bismarck brown
53
Carcinogenic [other stain]
Benzidine
54
Used as CHROMATIC STAIN for fresh materials in smear preparations. [other stain]
Carmine
55
Combined with aluminum chloride to stain glycogen (Carmine solution). [other stain]
Carmine
56
Used for routine staining of FIXED TISSUE. [other stain]
Celestine blue
57
Resistant to STRONG ACID DYES. [other stain]
Celestine blue
58
GOOD nuclear stain [other stain]
Celestine blue
59
Stains AMYLOID in frozen sections, platelets in blood. [other stains]
Crystal violet
60
Used for staining blood to differentiate WBC’s. [other stain]
Giemsa stain
61
Stain used for METALLIC IMPREGNATION. [other stain]
Gold sublimate
62
Made up of gold chloride and mercuric chloride. [other stain]
Gold sublimate
63
Oldest stain [other stain]
Iodine
64
For DEMONSTRATING MITOCHONDRIA during supravital staining. [other stain]
Janus Green B
65
Counterstain for ascaris eggs, RBC, bacterial spore stain (Schaeffer Fulton stain) [other stain]
Malachite green
66
Basic stain and Best vital dye. [other stain]
Neutral red
67
For demonstration of cell granules and vacuoles of phagocytic cells. [other stain]
Neutral red
68
Oxidizer of Tungsten Hematoxylin
Potassium permanganate
69
Mordant of Tungsten Hematoxylin
1% aqueous phosphotungstic acid
70
Recommended for DERMATOLOGICAL studies: [other stain]
Orcein
71
Fixative for EM [other stain]
Osmium tetroxide
72
Used to stain fat (black) [other stain]
Osmium tetroxide
73
Colored salt of ferric ferrocyanide. [other stain]
Prussian blue
74
Used for the MANUFACTURE OF PAINTS. [other stain]
Prussian blue
75
Used for identification of SPIROCHETES, reticulum, fiber stains. [other stain]
Silver nitrate
76
Used as nuclear stain in fixed tissue, stains NISSL GRANULES or chromophilic bodies. [other stain]
Toluidine nitrate
77
Demonstrates NEUROGLIA in frozen sections.
Victoria blue
78
[3] Staining method: Regressive [aluminum hematoxylin]
1. Harris hematoxylin 2. Ehrlich's hematoxylin 3. Mayer's hematoxylin

HPCT 311 (16 decks)

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