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HPCT 311 > H&E Staining, Mounting, Ringing and Labelling > Flashcards

H&E Staining, Mounting, Ringing and Labelling Flashcards

(73 cards)

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1
Q

Nuclear/basic/1’ stain

[h&e]

A

Hematoxylin

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2
Q

WALDEYER: 1st to use hematoxylin .

[h&e]

A

Hematoxylin

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3
Q

Most commonly used.

[h&e]

A

Eosin Y

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4
Q

Cytoplasmic/acidic/2’ stain

[h&e]

A

Eosin Y

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5
Q

Counterstain

[h&e]

A

Eosin Y

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6
Q

[3] Equipments

A
  1. Coplin jar
  2. Slotted staining dishes
  3. Metal/Glass staining racks
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7
Q

Holds 5-9 slides.

[equipment]

A

Coplin jar

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8
Q

Holds 5-19 slides.

[equipment]

A

Slotted staining dishes

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9
Q

Holds 10- 30 slides.

[equipment]

A

Metal/Glass staining racks

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10
Q

Use of a medium and a coverslip to facilitate the handling, storage, protection of the tissue section.

A

Mounting

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11
Q

To harden the medium:

A

Incubate 37˚C for 12-24hours after mounting.

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12
Q

Mounting medium should have a refractive index close to that of glass:

A

1.518

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13
Q

[2] Coverslip

A
  1. Coverslip 1.5 (180nm thick)
  2. Coverslip 1 (150nm thick)
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14
Q

Ideal for photomicrography.

A

Coverslip #1.5 (180nm thick)

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15
Q

Routinely used

[coverslip]

A

Coverslip #1 (150nm thick)

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16
Q

[2] Kinds of Mounting medium.

A
  1. Aqueous Mounting Medium
  2. Resinous Mounting Medium
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17
Q

Used to mount water miscible preparations .

[mounting medium]

A

Aqueous Mounting Medium

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18
Q

[4] Composition of Aqueous Mounting Medium

[GGSP]

A
  1. Gelatin, Glycerin Jelly, Gum Arabic
  2. Glycerol
  3. Sugar
  4. Preservative
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19
Q

Solidify the medium.

[composition of aqueous mm]

A

Gelatin, Glycerin Jelly, Gum Arabic

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20
Q

Prevent drying and cracking.

[composition of aqueous mm]

A

Glycerol

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21
Q

Increases refractive index.

[composition of aqueous mm]

A

Sugar

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22
Q

eg merthiolate

[composition of aqueous mm]

A

Preservative

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23
Q

[5] Aqueous Mounting Medium

[WG,FAB]

A
  1. Water
  2. Glycerin
  3. Farrant’s Medium (Gum Arabi Medium)
  4. Apathy’s Medium
  5. Brun’s Fluid
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24
Q

Temporary mounting

[aqueous mm]

A

Water

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25
Preservative [aqueous mm]
Glycerin
26
Refractive index: 1.46 [aqueous mm]
Glycerin
27
Refractive index of Glycerin
1.46
28
Standard mounting medium for FAT STAINS. [aqueous mm]
Glycerin
29
Sections are kept for years if sealed with paraffin. [aqueous mm]
Glycerin
30
Require ringing [aqueous mm]
Farrant's Medium (Gum Arabic Medium)
31
Methylene blue stained nerve preparations. [aqueous mm]
Apathy's Medium
32
Refractive index: 1.44 [aqueous mm]
Farrant's Medium (Gum Arabic Medium)
33
Refractive index of Farrant's Medium
1.44
34
Refractive index of Apathy's Medium.
1.52
35
Mounting FROZEN SECTIONS from water. [aqueous mm]
Brun's Fluid
36
[7] Resinous Mounting Medium [CDX, CPHC]
1. Canada Balsam 2. DPX 3. Xam 4. Clarite 5. Permount 6. HSR 7. Clearmount
37
Canada Balsam also known as?
Abus balsamea
38
Transparent and colorless but darkens and oxidizes with age. [resinous mm]
Canada balsam
39
For whole mounts and thick sections. [resinous mm]
Canada balsam
40
Refractive index: 1.524 [resinous mm]
Canada balsam
41
Refractive index of Canada balsam.
1.524
42
Diluents: Xylene, Toluene [resinous mm]
Canada balsam
43
Small tissue sections [resinous mm]
DPX
44
Dries rapidly [resinous mm]
DPX
45
Refractive index: 1.532 [resinous mm]
DPX
46
Refractive index of DPX.
1.532
47
Refractive index: 1.52 [resinous mm]
XAM
48
Refractive index of XAM
1.52
49
Synthetic resin mixture dissolve in xylene. [resinous mm]
XAM
50
Refractive index: 1.544 [resinous mm]
Clarite
51
Refractive index of Clarite.
1.544
52
Synthetic resin [resinous mm]
Clarite
53
Preferred over DPX. [resinous mm]
Clarite
54
Sealing the margins of the coverslip to prevent escape of fluid and evaporation.
Ringing
55
Prevents sticking of slides.
Ringing
56
[12] H&E staining steps [XDWWHA, AWEAXM]
1. Xylene 2. Descending grades of alcohol 3. Water 4. Washing out 5. Hematoxylin 6. Acid alcohol 7. Ammonia water 8. Water 9. Eosin Y 10. Ascending grades of alcholo 11. Xylene 12. Mount & label
57
Mounting medium is used:
Protects and prevents scratches from tissue sections.
58
[2] Most common sizes of Coverslip.
22x22 mm 20x20 mm
59
[2] Kinds of ringing media
1. Kronig cements 2. Durofix
60
2 parts PARAFFIN mixed with 4-9 parts powdered COLOPHONIUM RESIN. [ringing media]
Kronig cement
61
Cellulose adhesive [ringing media]
Durofix
62
Process of indicating the year and specimen number on one end of the prepared slide for proper identification.
Labelling
63
What is apathy's medium?
Methylene blue stained nerve preparations.
64
1st to use hematoxylin
Waldeyer
65
[10] Re-staining of old sections.
1. Slide 2. Xylene (24hrs) 3. Remove coverslip 4. Section: Xylene (30mins) 5. H2O 6. 0.5% K2MnO4 (5min) 7. H2O 8. 5% Oxalic acid (5 mins or until decolorized) 9. H2O 10. Restain
66
Filter out: every morning [h&e]
Hematoxylin
67
Primary stain [h&e]
Hematoxylin
68
Color of Hematoxylin
Purple
69
Color of Eosin Y
orang yellow/ red orange
70
Secondary stain [h&e]
Eosin Y
71
What is XAM?
Synthetic resin mixture dissolve in xylene
72
What is Kronig Cement?
2 parts PARAFFIN mixed with 4-9 parts powdered COLOPHONIUM RESIN
73
What is Canda Balsam?
For whole mounts and thick sections

HPCT 311 (16 decks)

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