Brainscape's Knowledge GenomeTM


Top Flashcards (Certified)
All Flashcards

bacteriology > Agars > Flashcards

Agars Flashcards

(80 cards)

Start Studying
1
Q

Differentiation using hemolytic properties

A

BAP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

BAP component?

A

Tryptic Soy Agar + 5% Sheep Blood/ 5% Horse Blood

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q
  • used to better isolate Streptococcus pneumoniae.
A

Gentamicin Blood Agar (GBA)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

GBA component?

A

TSA+ 5% sheep blood +gentamicin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Differential of MAC

A

Fermentation of lactose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

antibiotic inhibitor used to better isolate
Haemophilus spp.

A

Bacitracin-

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Makes use of horse blood (High concentration of X and V
Factor)

A

Bacitracin Blood Agar (BCA)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

MAC contains what to inhibit growth of gram pos bacteria?

A

Bile salts and crystal violet

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

V factor

A

NAD or NADP

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

CAP supplement?

A

VItalex

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

X factor?

A

Hemin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

H2S indicator of SSA?

A

Ferric citrate

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

indicator of SSA?

A

Neutral red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Indicator of Mac Conkey agar?

A

neutral red

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

A modified deoxycholate agar

A

Salmonella shigella agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Inhibitor of SSA

A

Bile salts, sodium citrate and brillant green

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

-Broth for the Isolation of Shigella and Salmonella

A

. SELENITE F broth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Medium for urine specimens?

A

BAP and MAC

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

-Strictly anaerobic bacteria (ie, those that cannot grow in the
presence of oxygen) grow at the bottom of the broth.

A

FLUID THIOGLYCOLLATE (FT)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Inhibitors of Thiosulfate citrate Bile Salt sucrose Agar?

A

Bile salt, Sodium cholate, alkaline pH

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Agar selective for vibrio spp

A

TCBS agar

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q
  • Used for antibiotic susceptibility testing (Disk Diffusion,
    test, Modified Hodge test, etc.) of most non-fastidious
    bacteria.
A

Mueller Hinton Agat

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Standard depth of MHA?

A

4mm

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

pH pf MHA?

A

7.2-7.4

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Indicator of TCBS agar?
Bromthymol blue
11
-General purpose medium that is routinely used to grow bacteria which tend to have high nutritional requirements (ce, they are fastidious)
TRYPTIC SOY BROTH (TSB)
11
broth medium for the enrichment of Vibrio species from food, water, and clinical samples.
ALKALINE PEPTONE WATER
12
- Used for antibiotic susceptibility testing of Haemophilus species.
HAEMOPHILUS TEST MEDIUM (HTM)
12
Medium for stool
MAC, SSA,TCBS, Selenite F, APW,
12
-This broth supports the growth of anaerobes, aerobes, microaerophilic, and fastidious microorganisms.
FLUID THIOGLYCOLLATE (FT)
13
Strict aerobic bacteria (ie, require oxygen for growth), such as Pseudomonas sp., tend to grow toward the surface of the broth
FLUID THIOGLYCOLLATE (FT)
14
-inhibitory for coliforms and certain other microbial specs, such as focal streptococci, present in fecal specimens
SELENITE F Broth
14
- Culture broth to grow aerobic and facultative anaerobic bacteria.
TRYPTIC SOY BROTH (TSB)
14
alkaline pH of this medium allows the growth of Vibrio organisms while inhibiting the growth of commensal intestinal bacteria.
ALKALINE PEPTONE WATER
15
medium for exudates?
BAP, MAC, FT
15
SIM: + for sulfide?
Black coloration
15
Medium for transudates?
BAP, CAP, MAC, TSB
15
Mediums for Respiratory specimens?
BAP, GBA,BCA,MAC
15
Triple Sugar Iron Agar (TSIA) compostion
0.1% glucose, 1% sucrose, 1% lactose
15
SIM; + for indole?
Reddish pink ring at junction
15
Indicator of TSIA?
Phenol Red
15
A/A?
Serratia Escherichia Enterobacter Klebsiella
16
SIM rgt?
Kovac's reagent (para-dimethylaminobenzaldehyde)
16
TSIA H2S indicator?
Ferrous sulfate
16
K/A:
Shigella Yersinia Citrobacter Providencia Proteus Yersinia
16
A/A H2S+?
Citrobacter Arizona Proteus
16
K/A H2S+
Citrobacter Arizona Salmonella Edwardsiella
16
Tests H2S production, indole reaction, and motility.
SIM (SULFIDE INDOLE MOTILITY)
16
K/K
Pseudomonas Acinetobacter
17
18
SIM: + for motility?
spread out/ movement away from the stab line/ hazy appearance
19
used to determine the ability of an organism to utilize sodium citrate as its only carbon source and inorganic ammonium salts as its only nitrogen source
CITRATE
20
Citrate slant: Carbon source?
Sodium citrate
21
Citrate slant: nitrogen source?
inorganic Ammonium salts
22
pH indicator of citrate?
Bromthymol blue
23
+ result for citrate?
green to blue slant
24
- used to determine whether a gram negative rod decarboxylates or deaminates lysine and forms H2S
Lysine Iron Agar
25
25
pH indicator of LIA?
Bromcresol Purple
26
Components of LIA?
Lysine, peptones, glucose, ferric ammonium citrate and sodium thiosulfate
27
Positive result for oxidase test?
Purple
27
LIA decarboxylation?
Butt
27
LIA Deamination?
Slant
27
-Tests a microorganisms ability of hydrolyzing urea to produce ammonia and carbon dioxide
Urease test
27
API (ANALYTICAL PROFILE INDEX) principle?
Turbidimetry
28
Urease test + color?
Pink color change
28
-To differentiate Enterobacteriaceae from non- Enterobacteriaceae
Oxidase test
28
-determine the sensitivity or resistance of pathogenic aerobic and facultative anaerobic bacteria to various antimicrobial compounds.
KIRBY-BAUER DISK DIFFUSION
28
Oxidase test detects the presence of?
Cytochrome oxidase
28
* Gram positive cocci Time to result
: 2 - 8 hours
29
- Automated Microbiology Bacterial Identification and antibiotic susceptibility system.
VITEK
29
- kits include strips that contain up to 20 miniature biochemical tests.
API (ANALYTICAL PROFILE INDEX)
29
negative oxidase test?
Escherichia, Klebsiella, Enterobacter, Serratia
30
Vitek cards contains __ with nutrients and biochemical test
64 wells
30
VITEK principle?
Colorimetry
31
Yeast like organisms: Time to result:
18 hours
32
Gran negative bacilli: Time to result:
2 - 10 hours
33
Gram positive spore forming bacilli: Time to result:
14 hours
33
* Anaerobic Bacteria Time to result:
6 hours
34
Coryneform bacteria Time to result
8 hours

bacteriology (24 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions