Partially Acid-Fast, Catalase Positive, non-motile

Question 1

The species of this genus are partially acid-fast, obligate aerobic, non-motile, and catalasepositive

Answer 1

Nocardia

Question 2

Nocardia cell wall contains peptidoglycan, meso-diaminopimelic acid (DAP), and the sugars,

Answer 2

arabinose and galactose

Question 3

The species grow on media that are used to recover fungi

Answer 3

Nocardia

Question 4

Gram-positive bacilli with long, thin, beaded, branching filaments

Answer 4

Nocardia

Question 5

Colonies exhibit wrinkled, chalk-like, and orange-tan pigmentation.

Answer 5

Nocardia

Question 6

Differential test of Nocardia

Answer 6

Resistant to lysozyme

Question 7

It is a chronic, localized, painless subcutaneous infection that is characterized by the
presence of sulfur granules in the affected tissue

Answer 7

Actinomycetoma (Actinomycotic mycetoma)

Question 8

Causative Agent of Actinomycetoma

Answer 8

Nocardia brasiliensis

Question 9

It is a confluent bronchopneumonia where the sputum is thick and purulent, although the
encapsulation of the abscess is absent
The affected tissues do not have sulfur granules

Answer 9

Pulmonary disease

Question 10

What are the best samples for Nocardia in performing wet mount and staining?

Answer 10

Pus and tissue specimen

Question 11

Causative Agent of pulmonary disease

Answer 11

: Nocardia cyriacigeorgica and Nocardia farcinica

Question 12

stain is used to observe a partially acid-fast Nocardia

Answer 12

Modified Ziehl-Nielsen or Kinyoun stain

Question 13

) stain is used for histopathological examination of
tissue specimens

Answer 13

Gomori‘s methenamine-silver (GMS) s

Question 14

is used to observe the morphology of actinomyctes, and to differentiate
branching Nocardia species from non-branching Rhodococcus species

Answer 14

Tap water agar

Question 15

These are aerobic, catalase-positive, branching, filamentous, Gram-positive bacteria that can
fragment into rods and cocci

Answer 15

Rhodococcus, Gordonia, and Tsukamurella

Question 16

It is non-motile and partially acid-fast, and is composed of mycolic acid with longer carbon
chains

Answer 16

Rhodococcus equi

Question 17

It can persist and replicate within macrophages

Answer 17

Rhodococcus equi

Question 18

It can infect immunocompromised individuals (HIV patients) and cause slowly progressive,
granulomatous pneumonia

Answer 18

Rhodococcus equi

Question 19

Microscopy: Coccobacilli with a ―”zigzag’ pattern and a filamentous form

Answer 19

Rhodococcus equi

Question 20

BAP-Colonies exhibit a pale pink or yeloow color

Answer 20

Rhodococcus equi

Question 21

The species of this genus vary from Gram-positive to Gram-variable rods
They are partially acid fast, non-motile and catalase-positive

Answer 21

Gordonia

Question 22

Colonies are smooth and slimy with irregular edges; but may appear as dry or rough;
and exhibit the presence of mycelia

Answer 22

Gordonia

Question 23

The species of this genus are Gram-positive and shaped like long rods that fragments into three
parts
 The members are slightly acid-fast when the kinyoun method is used

Answer 23

Tsukamurella

Question 24

Colonies are circular with rhizoid edges; has no aerial hyphae; and exhibit white or
orange pigmentation

Answer 24

Tsukamurella