Rickettsiaceae

Question 1

Small (0.5 – 2 um) non-motile, pleomorphic bacilli, have a g(-) cell wall

Answer 1

RICKETTSIACEAE

Question 1

It is the simplest
bacterial form and
considered transitional
organism between
bacteria and viruses

Answer 1

RICKETTSIACEAE

Question 2

This group of organisms
infect wild animals, with
humans acting as
accidental hosts

Answer 2

RICKETTSIACEAE

Question 3

all species require living cells for growth except for

Answer 3

Bartonella quintana

Question 4

small organism, pleomorphic, gram-negative bacilli and multiplies by binary fission in the
cytoplasm of host cells (the release of mature rickettsiae results in the lysis of the host cell)

Answer 4

Rickettsia

Question 5

survives only briefly outside of a host and multiply only intracellularly in the cytosol of the host
cell

Answer 5

Rickettsia

Question 6

Rickettsia :
Lysis of cell wall

Answer 6

Rickettsia prowazekii

Question 7

Rickettsia:
Filopodium focal lysis

Answer 7

Ricketssia rickettsii

Question 8

Rickettsia:
Budding

Answer 8

Rickettsia tsutsugamushi

Question 9

contributes to its intracellular activity.

Answer 9

Phospholipase A2

Question 10

can be passed from generation to generation of ticks through their eggs (transovarian
passage)

Answer 10

Rickettsia ricketsii

Question 11

Ricketssia Groups of Bacteria

Answer 11

Spotted fever group
Typhus Group
Scrub typhus group

Question 12

They grow in the cytoplasm of host cell and released via ―pinching‖ off‖ a host cell membranebound rickettsia

Answer 12

Orientia tsutsugamushi

Question 12

• It was placed into a separate genus due to the absence of LPS and peptidoglycan, and the
  presence of 54 – 58 kDa major surface proteins

Question 13

It is transovarially maintained in mites (Leptotrombidium

Answer 13

Orientia tsutsugamushi

Question 14

Humans and rats are accidental, nonessential dead-end hosts

Answer 14

Orientia tsutsugamushi

Question 15

Small (0.5 um, gram negative coccobacilli and undergo intracellular development cycle following
infection of circulating WBC

Answer 15

Ehrlichia

Question 16

nfection of circulating WBC
- They undergo 3 developmental stages similar to Chlamydiae: EB, initial bodies and morulae

Answer 16

Ehrlichia

Question 17

3 Developmental stages of Erlichia

Answer 17

Elementary body, Initial body, Morulae

Question 18

Wright-Giemsa staining of intravacuolar microcolony resembles?

Answer 18

“mulberry” morula

Question 19

Infects monocytes and causes HME (Human Monocytic Ehrlichiosis)

Answer 19

Erlichia Chaffeensis

Question 20

It is transmitted to humans by the lone star tick (Amblyomma americanum)

Answer 20

Erlichia Chaffeensis

Question 21

Can also be transmitted by D. andersoni and Ixodes pacificus

Answer 21

Erlichia Chaffeensis

Question 22

Causes HGE (Human Granulocytotropic Ehrlichiosis)

Answer 22

Erlichia phagocytophila

Question 23

Transmitted by I. pacificus, I. scapularis, and I. ricinus ticks, and white footed mouse (Peromyscus leucopus)

Answer 23

Erlichia phagocytophila

Question 24

This is the causative agent of Q fever – a systemic infection that affects the lungs

Answer 24

Coxiella burnetti

Question 25

- Strictly intrancellular, gram-negative bacteria, smaller that Rickettsia species; more resistant to chemical and physical agents, dessication and sunlight (able to withstand harsh environmental conditions)

Answer 25

Coxiella burnetti

Question 26

Can survive extracellularly because of its endospore-like body – can be grown only in lung cells

Answer 26

Coxiella burnetti

Question 27

In infected animals, organisms are shed in urine, feces, milk and birth products - Humans are infected by the inhalation of contaminated aerosols from dried animal feces

Answer 27

Coxiella burnetti

Question 28

In contrast to rickettsial infection, a rash does not develop following infection

Answer 28

Coxiella burnetti

Question 29

In contrast to rickettsial infection, a rash does not develop following infection - It does not multiply in bacteriologic culture media

Answer 29

Coxiella burnetti

Question 30

Shell vial assay with human lung fibroblasts is used to isolate the organism from the buffy coat and biopsy specimens has not resulted in any laboratory acquired infections

Answer 30

Coxiella burnetti

Question 31

serologic techniques for detection of Coxiella burnetti

Answer 31

IFA, CF, EIA

Question 32

Animal reservoir – cattle, sheep and goats

Answer 32

Coxiella burnetti

Question 33

Facultative intracellular gram-negative bacilli - Do not synthesize acid from carbohydrates

Answer 33

Bartonella

Question 34

They live within red blood cells in their natural mammalian hosts

Answer 34

Bartonella

Question 35

They can be cultivated in blood enriched with 5% CO2 (CAP) or charcoal yeast extract agar (CYEA)

Answer 35

Bartonella

Question 36

Trench fever is transmitted from person to louse (Pediculus humanus corporis) to another person.

Answer 36

Bartonella

Question 37

Bartonella species: negative catalase, oxidase and urease

Answer 37

Bartonella henselae

Question 38

Bartonella that is an etiologic agent of trench fever

Answer 38

Bartonella quintana

Question 39

Bartonella that is an etiologic agent og infective endocarditis

Answer 39

Bartonella elizabethae

Question 40

Bartonella that is an etiologic agent of Oraya fever (chronic verruga peruana) and febrile acute hemolytic anemia

Answer 40

Bartonella bacilliformis

Question 41

for the detection of morulae during the febrile stage of Ehrlichiosis

Answer 41

Giemsa or Diff-Quik stains

Question 42

agglutination of certain strains of Proteus vulgaris by serum from patients with rickettsial diseases; presumptive test

Answer 42

Weil-Felix reaction

Question 43

– excellent sensitivity for detecting antibodies to rickettsia; early diagnosis of RMSF within 7 – 10 days after onset of symptoms

Answer 43

Microimmunofluorescent Dot test

Question 44

– do not induce Weil-Felix antibody in infected patients - Antibodies to rickettsia (except R. rickettsia) cannot be reliably detected until at least 2 weeks after the patient has become ill

Answer 44

- Q fever, Ehrlichiosis and Rickettsial pox