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Strep Flashcards

1
Q

fermenters but not gas producers

A

Strep and entero

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2
Q

Grow poorly in nutrient agar and tryptic soy broth

A

Strep and entero

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3
Q

• young-cultures have capsule in cell morphology

A

Strep

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4
Q

grayish, pinpoint, translucent to slightly opaque
• shows mucoid colonies

A

Strep

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5
Q

• some are aerotolerant
o microorganisms that grow in presence of oxygen but
unable to use oxygen for metabolism

A

Strep

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6
Q

Most notorious species – most clinically important species of strep?

A

Streptococcus pyogenes
• Streptococcus pneumoniae

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7
Q

o members of streptococcus that shows growth at 37°C
o usually produces rapidly
o mostly beta-hemolyt

A

Pyogenic Group

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8
Q

growth at 37°C and 45°C
o species: Enterococcus faecalis
o mostly normal inhabitants of the upper respiratory tract

A

Viridans group

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9
Q

growth at lower temperatures (10°C & 37°C)
o often found in dairy products
o species: Streptococcus lactis
▪ an organism that can cause normal coagulation
of milk or dairy products which leads to soaring

A

Lactococci

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10
Q

10°C, 37°C, & 45°C)
o wide range of temperature where they can show growth

A

Enterococcus

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11
Q

it is encapsulated even the old culture
which is a protection from the extraction of
the C-carbohydrate from the cell wall

A

S.pneu

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12
Q

Strep that - does not belong to any Lancefield
classification

A

S.pneu

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13
Q

Strep? M protein is attached to the peptidoglycan of the cell wall
and extends to the cell surface

A

Streptococcus pyogenes or Group A Streptococcus (GAS)

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14
Q

Strep pyo: is the most common serotype seen in
pharyngitis

A

M1 serotype

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15
Q

In strep pyo, this causes inflammation of oral cavity

A

M1 serotype

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16
Q

Strep pyo: are adhesion molecules that
mediate attachment to host epithelial cells.

A

Fibronectin-binding protein (Protein F) and
Lipoteichoic acid

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17
Q

Strep pyo: prevents opsonized
phagocytosis by neutrophils or macrophages. It also
allows the bacterium to mask its antigens and remain
unrecognized by its host

A

Hyaluronic acid capsule

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18
Q

Strep pyo: responsible for hemolysis on Sheep
Blood Agar plates incubated anaerobically

A
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19
Q

Strep pyo: oxygen-labile exotoxin

A

o Streptolysin O –

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20
Q

Strep pyo; responsible for the beta-hemolysis observed on
blood agar plates that are incubated
anaerobically
▪ causes subsurface hemolysis

A

streptolysin O

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21
Q

Strep pyo: lyses leukocytes and is non-
immunogenic

A

Streptolysin S

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22
Q

is an enzyme that
solubilizes the ground substance of mammalian
connective tissues
▪ allows the movement of organisms in tissues

A

Hyaluronidase

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23
Q

Strep pyo: red spreading rash
▪ contributes to the invasiveness of the
microorganism

A

Streptococcal pyrogenic endotoxins/erythrogenic
toxins

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24
Q

Strep throat is most often seen in children between 5
and 15 years of age

A

Strep pyo

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25
Skin or pyodermal infections include impetigo, cellulitis, erysipelas, wound infection, or arthritis
Strep pyo: pyodermal infections
26
invasive infection characterized by rapidly progressing inflammation and necrosis of the skin, subcutaneous fat, and fascia o rapid progression of inflammation and skin necrosis o flesh-eating bacteria
Strep pyo: Necrotizing Fasciitis (Galloping gangrene
27
Entire organ system shuts down o Root cause: strep throat that was not managed immediately
Strep pyo: Streptococcal Toxic Shock Syndrome
28
a group of immune-mediated complications that usually occurs following a streptococci infection
sequelae
29
2 types of Poststreptococcal sequelae: an inflammatory condition that can affect different parts of the body
Rheumatic fever
30
2 types of Poststreptococcal sequelae: inflammatory disease of the renal glomeruli - caused by the deposition of antibody and antigen complex in the glomerulus
Acute Glomerulonephritis/Bright's disease –
31
a punctuate exanthem overlying diffuse erythema o communicable disease
Scarlet Fever (Scarlatina)
32
diffused red rash on the upper chest spread to the trunk and extremities ▪ “strawberry-colored tongue” - caused by competition
Strep pyo: Scarlet Fever (Scarlatina)
33
Anti-streptolysin O Test/Titer Test/ASO Titer
Dick’s test
34
Diagnostic test for scarlet fever
Schultz-Charlton Test
35
Screening test to identify group A
 Streptococcus
Bacitracin Disk test/Taxo A (0.04 units
36
identifies Group A and B Streptococci o Positive result: Group A and B are resistant
Sulfamethoxazole and Trimethoprim test (SXT)
37
o Positive result of PYR test
Cherry red color
38
Acid stable polysaccharide is located in the cell wall of what strep group?
Streptococcus agalactiae or Group B Streptococcus (GBS)
39
GBS: ▪ Serotypes Ia, IIb and II contain a terminal residue of
Sialic acid
40
The capsule prevents phagocytosis but is ineffective after opsonization
GBS
41
GBS: appears to be the most significant component of the capsule and a critical virulence determinanT
Sialic acid
42
used to differentiate Group A from Group B Strep
CAMP test
43
CAMP positive result
Arrow-head zone of B-hemolysis near the growth of S. aureus
44
utilized to detect the presence of the enzyme hippuricase or hippurate hydrolase
Hippurate hydrolysis test
45
Group that possesses the enzyme Hippuricase or Hippurate hydrolase
GBS
46
First rgt of HH test
Sodium hippurate
47
Second rgt of HH test
Ninhydrin
48
Product of HH test
Hippuric acid
49
Positive reaction of Hh test
Purple coloration of the media after adding Ninhydrin reagent
50
Most useful biochemical test for GBS
o Hippurate Hydrolysis o CAMP Test
51
GBS sample for pregnant women should be inoculated into selective broth such as?
Todd-Hewitt broth containing 10 ug/mL colistin and 15 ug/mL nalidixic acid
52
• Neonatal Streptococcal Infection is caused by?
GBS or step agalactiae
53
is a normal flora of the female genital tract
GBS
54
gram positive cocci in pairs, oval, lancet shape, and facultative anaerobe
D. Streptococcus pneumoniae (Diplococcus/Pneumococcus)
55
The cell wall of S. pneumoniae contains an antigen, referred to as
C.substance
56
In the presence of specific anticapsular serum, the capsule swells called?
Quellung reaction for S.pneu
57
most useful o Principle: In the presence of specific anticapsular serum, the capsule swells
Neufeld Quellang Test (Capsular swelling test)
58
Diseases caused by S. pneumoniae: mainly affects one or more lobes of the lungs o bloody rust tinge sputum with large number of WBC o alveoli are infected
Lobar pneumonia
59
They were previously classified as group D streptococci.
Entero
60
sometimes exhibit pseudo catalase reaction o weak/minimal bubbling
Enterococci
61
They were previously classified as group D streptococci. • Natural inhabitants of the intestinal tracts of human and animals
Entero
62
Can grow in extreme conditions: Alkaline pH, high temperatures up to 45°C, increased salt concentrations
Entero
63
Known to be resistant to multiple antimicrobial age
Entero
64
a type of bacteriocin found in gram positive bacteria and usually important in quorum sensing
Cytolysin
65
medium containing tellurite is recommended for
E.faecalis
66
Mostly known to cause subacute bacterial endocarditis
Viridans Streptococci
67
Viridans strep is bile soluble or insoluble?
Insoluble
68
Viridans strep is optochin resistant or susceptible?
Resistant
69
presence of LAP is a characteristic of?
Viridans strep

bacteriology (24 decks)

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