Chkamydiaeceae

Question 1

They contain both the DNA and RNA, ribosomes and they produce their own proteins, lipids and
nucleic acids
o They were once thought to be large viruses, but they contain both DNA and RNA

Answer 1

CHLAMYDIACEAE

Question 2

Chlamydiaceae stain poorly with gram stain, but they could be better visualized with the

Answer 2

Gutsein Stain

Question 3

Non-motile, small (0.2 – 1.5um), resembles gram-negative cell wall, obligate intracellular
bacteria that requires living cells for growth

Answer 3

CHLAMYDIACEAE

Question 4

Requires the biochemical resources of the eukaryotic host cell‘s environment to fuel their
metabolism for growth and replication – “energy parasite”

Answer 4

CHLAMYDIACEAE

Question 5

They have tropism for columnar epithelial cells

Answer 5

CHLAMYDIACEAE

Question 6

Posses a heat stable, family specific antigen that is an essential component of the cell
membrane (LPS

Answer 6

CHLAMYDIACEAE

Question 7

Morphologic forms of Chlamydiaceae:
Replicative form
Intracellular and metabolically active

Answer 7

Reticulate body

Question 8

Morphologic forms of chlamydiaceae:
Inefective form
Extracellular and structurally resembles a gram-negatice bacillus
Have a rigid cell wall

Answer 8

Elementary body

Question 9

2 components of the outer membrane of elementary body

Answer 9

MOMP( major aouter membrane protein and lipopolysaccharide (LPS) antigen

Question 10

• Major cause of pelvic inflammatory disease(PID) and ocular trachoma

Answer 10

Chlamydia trachomatis

Question 11

• Major cause of pelvic inflammatory disease(PID) and ocular trachoma

Answer 11

Chlamydia trachomatis

Question 12

Serotype of endemic trachoma

Answer 12

Serotypes A, B, Ba, C

Question 13

serotype of lymphogranuloma venereum (LGV)

Answer 13

Serotypes L1, L2, L3

Question 14

serotype of PID, urethritis, cervicitis, epididymitis, inclusion conjunctivitis

Answer 14

Serotypes D- K

Question 15

Associated with infertility and ectopic pregnancy

Answer 15

Chlamydia trachomatis

Question 16

Intraperitoneal spread may cause peritonitis or perihepatitis

Answer 16

(Fitz-Hugh-Curtis syndrome)

Question 17

Chronic inflammation of the conjunctiva leading to blindness
o It can cause distortion of the eyelids – the eyelashes become misdirected and turn in
o It is transmitted by contact with individuals that carries the organism from an infected
eye, or by flies

Answer 17

Trachoma

Question 18

A sexually transmitted disease and has multisystem involvement
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o A small painless ulcer or papule appears initially and nodules (buboes) develop after
several weeks
o Fret‘s test – intradermal skin test

Answer 18

Lymphogranuloma Venereum (LGV

Question 19

It is characterized by copious discharge from the eye, an inflamed and swollen
conjunctiva, and the presence of large inclusion bodies in the host cell cytoplasm
o Usually affects infants

Answer 19

Inclusion conjunctivitis

Question 20

Genus specific antigen can be detected by this methd
 Used to diagnose LGV but not trachoma, inclusion conjunctivitis and
neonatal infection
 Single point titer > 1:64

Answer 20

 Complement fixation

Question 21

Used for type-specific antibodies of C. trachomatis
 Can be used for diagnosis of LGV, trachoma, inclusion conjunctivitis and
neonatal infection
 IgM titer of 1:32

Answer 21

Microimmunofluorescence assay (Micro-IF)

Question 22

RAtio of single point titer

Answer 22

> 1:64

Question 23

Ratio of IgM titer

Answer 23

1:32

Question 24

Etiologic agent of psittacosis/ornithosis

Answer 24

Chlamydophila (formerly Chlamydia) psittaci

Question 25

Endemic pathogen of all bird species – parrots, parakeets, chicken, ducks

Answer 25

Chlamydophila (formerly Chlamydia) psittaci

Question 26

Outbreaks have occurred among turkey-processing workers and pigeon aficionados
- Humans acquire the disease by inhalation of infected aerosols form dried bird excreta or
handling of infected birds
- only laboratories with biosafety level 3 facilities can perform cultivation of samples

Answer 26

Chlamydophila (formerly Chlamydia) psittaci

Question 27

widely used serologic test to detect psittacosis

Answer 27

. complement fixation

Question 28

sensitive method

Answer 28

Direct Microimmunofluorescence

Question 29

identity and distinguish all 9
chlamydial species including C. psittaci

Answer 29

Amplification of rDNA using PCR followed by RFLP

Question 30

human pathogen
- transmission is by aerosol droplets via the respiratory route
- “pear -shaped” appearance of EB; has round and dense inclusions

Answer 30

Chlamydophila (formerly chlamydia) pneumoniae (TWAR strain)

Question 31

resistant to sulfonamides
- negative for glycogen staining of inclusion bodies
- uses HL of Hep-2 cell lines for culture

Answer 31

Chlamydophila (formerly chlamydia) pneumoniae (TWAR strain)