B cells encounter Ag

Question 1

B cell home to LN via what

Answer 1

HEV

Question 2

what do b cells use to enter t cell zone

Answer 2

CCL21 and CCL19

Question 3

what do b cells use to move to the b cell zone

Answer 3

CCL13

Question 4

where do b cells move to to interact with macrophage

Answer 4

b cells move subcapsular sinus to interact with macrophage

Question 5

once b cells move to subcapsular sinus to interact with macrophage and it binds ag what happens

Answer 5

b cells express CD69 to retain S1PR inside the cells-> prevents B cells from leaving LN.

Question 6

is B cells dont interact with the macrophage it will move to

Answer 6

follicle and interacts with FDC

Question 7

if b cell moves to follicle and interacts with FDC and binds Ag what happens

Answer 7

b cells express CD69 to retain S1PR inside the cells-> prevents B cells from leaving LN.

Question 8

if the b cell moves to follicle and interacts with FDC and doesn’t bind Ag what happens

Answer 8

B cell follows S1P gradient and leaves LN. and goes back to the beginning

Question 9

Once B cells express CD69 to retain S1PR inside the cells and prevents B cell from leaving LN what happens next

Answer 9

B cell endocytose Ag and present it on MHCII

Question 10

once the B cell endocytose Ag and present it on MHCII what happens

Answer 10

B cell expresses CCR7 and moved to B/T cell boundary

Question 11

once the B cell expresses CCR7 and moved to B/T cell boundary what happens

Answer 11

B cell forms a cognate pair with TFH cell which bind both the same Ag

Question 12

once B cell forms a cognate pair with TFH cell what happens

Answer 12

CD40L binds CD40-> induces NF-KB

Question 13

NF-KB does what

Answer 13

upregulates adhesioin molecules

Question 14

Once CD40L binds CD40-> induces NF-KB what happens

Answer 14

B cell expresses ICAM-1 which binds LFA-1 on the T cell

Question 15

once B cell expresses ICAM-1 which binds LFA-1 on the T cell what happens

Answer 15

TFH cell delivers cytokines directly on the surface of the B cell

Question 16

once TFH cell delivers cytokines directly on the surface of the B cell what happens

Answer 16

B cell proliferate to form primary focus

Question 17

what is subset 1 once b cells proliferate to form primary focus

Answer 17

B cell becomes IgM plasma cell and secretes IgM

Question 18

what is subset 2 once b cells proliferate to form primary focus

Answer 18

continue differentiation

Question 19

once B cells proliferate to form primary focus what happens

Answer 19

B cells move to primary follicle

Question 20

what happens in the primary follicle

Answer 20

becomes germinal center

Question 21

what does the light zone contain

Answer 21

FDCs B cell undergoes affinity maturation

Question 22

what survives in affinity maturation

Answer 22

strongest binder survives

Question 23

what is the dark zone

Answer 23

densely populated and is where B cells proliferate and undergo somatic hypermutation

Question 24

what cells use somatic hypermutation

Answer 24

TFH

Question 25

process of somatic hypermutation

Answer 25

activate AID-> which introduces mutations and then each proliferate the B cell and gets differentiation mutations and if they express mutated BCR they move to the light zone

Question 26

affinity maturation uses what cells

Answer 26

FDCs

Question 27

what are in affinity maturatioin but are not quite a plasma cell

Answer 27

centrocytes

Question 28

in affinity maturation: bind better to Ag results in

Answer 28

survival signals from FDC

Question 29

in affinity maturation: bind equal to Ag results in

Answer 29

die by apoptosis

Question 30

in affinity maturation: bind less to Ag results in

Answer 30

die by apoptosis

Question 31

in affinity maturation: bind self results in

Answer 31

induce anergy

Question 32

what cells are used in class switching

Answer 32

TFH

Question 33

what do TFH cells secrete in class switching

Answer 33

cytokines

Question 34

When TFH cells secrete cytokines in class switching what is it doing

Answer 34

tell B cell which Ab to make

Question 35

low level transcription in class switching does what

Answer 35

opens up locus

Question 36

what mediates class switching

Answer 36

AID

Question 37

what does class switching require

Answer 37

CD40-CD40L interacting signals

Question 38

CD40L deficiency results in

Answer 38

hyper IgM syndrome

Question 39

hyper IgM syndrome results in

Answer 39

not being able to make germinal centers and only IgM plasma cells

Question 40

after B cell moves to primary follicle what happens

Answer 40

B cell differentiates into plasma cell and memory B cells

Question 41

plasma cells stay where and secrete what

Answer 41

in lymph node and secrete Ab

Question 42

are plasma cells short or long lives

Answer 42

short

Question 43

when plasma cells move to bone marrow and secrete Ag are they long or short lived

Answer 43

long lived

Question 44

memory b cells are very long or short lived

Answer 44

long

Question 45

memory b cells differentiate into

Answer 45

plasma cells after interaction with Ag