BACTE LAB LA2 Flashcards
deals with detecting, culturing, and identifying microorganisms like bacteria, fungi, viruses, etc.
MICROBIOLOGY LABORATORY
Example of Obligate Anaerobes
- Clostridium
- Bacteroides
- Fusobacterium
- Pepto streptococcus
also known as tissue culture hoods, are
designed to provide personnel, environmental and product protection when
appropriate practices and procedures are followed
Biological Safety Cabinets
BSC certification validity
12 months
Operate the BSC _________ for at least ______________ before beginning work to
allow the BSC to “purge” particulates
blowers, 3-5 minutes
Disinfect all interior surfaces of BSC work area with an appropriate surface
disinfectant which include:
10% bleach followed by 70% ethanol
Perform all operations at least _____________ from the front grille on the work surface.
4 inches
is a spill that is one that the laboratory staff is capable of handling safely without the assistance of safety and emergency personnel
MINOR SPILL
In minor spill Allow disinfectant _______________ of contact time before wiping up the spill.
20 minutes
In minor spills Allow BSC to run an additional ___________ before resuming work or shutting down.
10 minutes
is a spill requires active assistance from emergency personnel.
MAJOR SPILL
are commonly used in healthcare settings to sterilize medical devices.
AUTOCLAVES
The items to be sterilized are placed inside an autoclaves’ pressure vessel or is commonly referred to as the ____________
chamber
How often is the checking for AUTOCLAVE timing
Quarterly
Three factors are critical to ensuring successful steam sterilization in an autoclave:
time, temperature and steam
quality
3 Phases of Autoclave Process
Conditioning Phase
Exposure Phase
Exhaust Phase
Air inhibits sterilization and must be removed from the chamber during the first phase of the sterilization cycle known as ______________________
Conditioning
use steam to displace the air in the chamber and force
the air down the sterilizer drain.
Gravity-type sterilizers
After the air is removed, the sterilizer drain closes and steam is continuously admitted into the chamber, rapidly increasing the pressure and temperature inside to a predetermined level. The cycle enters the ________________ and items are held at the sterilization temperature for a fixed amount of time required to sterilize them.
Exposure Phase
During the final phase of the cycle, the sterilizer drain is opened and steam is removed, depressurizing the vessel and allowing the items in the load to dry.
Exhaust Phase
are designed to validate that an autoclave run was successful at killing microorganisms
Biological indicator systems
__________________________ spores are highly resistant to steam and vaporized hydrogen peroxide and are therefore most commonly used in biological indicator tests.
Geobacillus stearothermophilus
are used to validate whether an autoclave is working properly.
Chemical and biological indicators
are quick to read, but generally, only demonstrate that the autoclave has reached a certain minimum temperature.
Chemical indicators
