Basic Bacteriology I Flashcards

(54 cards)

1
Q

MOA penicillin

A

bind and inhibit transpeptidase

-inhibit crosslinking peptidoglycan layer

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2
Q

transpeptidase

A

PCN binding protein

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3
Q

lipid A

A

induces TNF and IL-1

O polysaccharide is antigen

endotoxin - lipid A

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4
Q

protein synthesis in bacteria

A

50S and 30S ribosomes

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5
Q

gram negative

A

appear blue

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6
Q

gram negative

A

appear red

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7
Q

plasmid in bacteria

A

contains genes for antibiotic resistance, enzymes, toxins

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8
Q

bacillus anthracis capsule

A

D-glutamate

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9
Q

glycocalyx

A

mediate adherence in bacteria - to foreign surface - indwelling catheter

loose network of polysaccharides

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10
Q

unique to gram positive

A

lipotechoic acid

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11
Q

unique to gram negative

A

porin
endotoxin - LPS - outer membrane
periplasmic space - location of beta-lactamase

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12
Q

outer membrane of gram negative

A

has LPS - endotoxin

O polysaccharide - antigen
lipid A - induce TNF and IL1

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13
Q

gram positive cell wall

A

outer to inner

thick peptidoglycan
cytoplasmic membrane

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14
Q

gram negative cell wall

A

outer to inner

outer membrane - with LPS
thin peptidoglycan layer
periplasmic space
cytoplasmic membrane

also - has porins

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15
Q

gram + cocci

A

staph
strep
enterococcus

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16
Q

gram - cocci

A

moraxella catarrhalis
neisseria

both - diplococci

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17
Q

gram + rod spore formers

A

bacillus

clostridum

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18
Q

gram + rod non-spore formers

A

cornybacterium

listeria

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19
Q

short rod

A

coccobacilli

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20
Q

mycoplasma cell wall

A

only simple cell membrane

-don’t stain gram + or -

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21
Q

mycobacterium stain

A

weakly gram +

stain acid fast

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22
Q

enteric gram - rods

A
live in GI tract
bacteroides
campylobacter
E. coli
enterobacter
helicobacter
klebsiella
proteus
pseudomonas
salmonella
shigella
serratia
vibrio
yersinia
23
Q

respiratory gram - rods

A

bordetella
haemophilus
legionella

24
Q

silver stain

25
zoonotic gram - rods
bartonella brucella francisella pasteurella
26
other gram + rods
gardnerella lactobacillus mycobacterium propionibacterium
27
branching filament gram +
actimoyces | nocardia
28
obligate intracellular gram -
chlamydia and rickettsia
29
spiral gram -
spirochetes borrelia, leptospira, and treponema
30
no cell wall
mycoplasma | ureaplasma
31
dark field microscopy
treponema - too thin to be visualized on gram stain
32
mycobacterium gram stain
high lipid content | mycolic acid in cell wall - detected by carbolfuschin in acid-fast stain
33
giemsa stain
``` chlamydia borrelia rickettsia trypanosome plasmodium ```
34
PAS stain
stain glycogen diagnosis of whipple disease - tropheryma whipplei
35
ziehl neesen stain
carbol fuschin acid fast - nocardia and mycobacterium protozoa
36
india ink stain
cryptococcus neoformans
37
silver stain
fungi legionella H. pylori
38
intracellular parasite bacteria
gram stain doesn't work | chlamydia and rickettsia
39
chocolate agar
h. flu
40
thayer martin agar
stain neisseria - gonorrhea and meningitis vancomycin - inhibit gram + trimethoprim colistin - inhibit gram - except neisseria nystatin - inhibit fungi
41
borget-gengou agar
bordetella pertussis
42
tellurite agar
c. diptheria
43
lowenstein jensen agar
mycobacterium tuberculosis
44
eaton agar
mycoplasma pneumonia requires cholesterol
45
macconkey agar
lactose fermenting enterics fermentation produces acid - causes colonies to turn pink
46
charcoal yeast extract agar buffered wth cystein and iron
legionella
47
sabouraud agar
fungi
48
EMB agar
E. coli eosin methylene blue green metallic sheen
49
aerobes
use O2 dependent systems - generate ATP | -nocardia, pseudomonas aeruginosa, m. tuberculosis
50
reactivation TB location
apices of lung | -highest O2
51
anaerobes
fusobacterium, clostridium, bacteriodes, actinomyces -lack catalase and superoxide dismutase - oxidative damage aminoglycosides - don't work - require O2 to enter bacterial cell
52
catalase
2H2O2 > 2H2O + O2
53
superoxide dismutase
O2- + O2- + 2H+ > H2O2 + O2
54
periplasmic flagella
in spirochetes | -run sideways along outer membrane sheath