Basic Hematologic Techniques Flashcards Preview

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Flashcards in Basic Hematologic Techniques Deck (20):
1

Describe the process of getting a PCV

Fill microhematocrit tube 70-90% 

Plug one end with clay

Place in centrifuge

Remove from centrifuge

2

How does a refractometer work?

Solute bends light passing through the fluid, refraction is proportionate to teh solute concentration

3

Solutes that will interfere with refractometer readings (artificially increase TP)

Lipemia

Urea

Glucose

Cholesterol

4

If PCV and TP are proportionally increased then the patient 

is dehydrated

5

If the PCV and TP are proportionally decreased then the patient 

has blood loss

6

Two major constituents of Total Protein

Albumin

Globulin

7

Increased TP, Globulin and Albumin

Dehydration

8

Increased TP and Globulin

Inflammation or Neoplasia

9

Decreased TP, Albumin and Globulin

Blood Loss

Protein Losing Enteropathy

10

Decreased TP and Albumin

Protein Losing Nephropathy

Liver Failure

Vaculitis

11

Decreased TP and Globulin

FPT

SCIDS

12

Stained blood films are essential for

Determining concentration of individual leukocyte types

Evaluating morphologic abnormalities of leukocytes, erythrocytes and platelets

13

Stains used to stain a blood slide

Wright Stain

Wright Giemsa Stain

Diff Quik

14

Systemic Blood Film Evaluation steps

  1. 10x lens - Scan feathered edge
  2. 10x lens - Examine monolayer
  3. 100x lens - Evaluate monolayer

15

What should you be looking for on 10x when looking at the feathered edge?

Platelet clumps

Organisms

16

What should you be looking for on 10x looking at the monolayer?

Estimating RBC and WBC count

17

On 100x looking at the monolayer what should you be doing?

Evaluating RBC, WBC (differentiating) and platelets 

18

RBC evaluation should involve

Size

Color

Shape

Arrangement

Inclusions/Organisms

19

WBC evaluation should involve

Differential cell count

Leukocyte morphology

20

Macroplatelets / Giant Platelets

Term used to describe platelets that are near the size of  RBCs