Biol 445 final material Flashcards Preview

Spring 2013 > Biol 445 final material > Flashcards

Flashcards in Biol 445 final material Deck (37):
1

MDM2

and E3 ubiquitinase that destroys p53

2

p53: tumor suppressor or oncogene?

tumor suppressor

3

E2F's effect on cyclin E

produces more of it

4

Cyclin E

hyperphosphorylates Rb later (CDK2)

5

Cyclin D

hypophosphorylates Rb early on (CDK 4/6)

6

Hyper-phosphorylated Rb

is free from E2F
recruits histone acetylase

7

Un/Hypo-phosphorylated Rb

stays bound to E2F
recruits histone deacetylaces

8

Rb regulates

progression through G1

9

biological role of abl

phosphorylates focal adhesion proteins and regulators of the actin cytoskeleton
responds to DNA damage

10

homologous abl

arg

11

abl structure is similar to?

src

12

Oncogenic abl mutation (in what domain?)

Myristate; carboxy terminus
Allows for "off" formation

13

bcr/abl fusion protein effects

increased proliferation/differentiation
increased stem cell turnover
decreased apoptosis
creates cytoskeletal/adhesion effects

14

Philadelphia chromosome

t9;22 causes CML and present in some adult ALLs
fuses bcr and abl genes

15

TCF4

turns Wnt pathway off
used by Clevers to find genes turned on by Wnt are in crypts
and genes turned off by Wnt are in villi

16

TCF

together with armadillo activates Wnt-responsive genes

17

armadillo

normally is phosphorylated by Fz
without phosphorylation, moves to nucleus and binds to TCF

18

wingless (wnt)

induces segmentation
places of expression take on anterior fate
binds Fz

19

p95 is also known as

beta-catenin; hedgehog

20

FAP genetic cause

APC gene

21

apoptosome

activates caspases

22

apaf bound to cytochrome-c:

assembles apoptosome

23

cytochrome-c

binds to apaf

24

bcl-2 causes:

cytochrome-c to leave mitochondria membrane
opens pores in mitochondrial membrane

25

bcl-2 equivalent to

ced-9

26

ced-3

results in apoptosis

27

ced-4

activates ced-3

28

ced-9

inhibits ced-4

29

p21 function

Forms inhibitory complex between CDK2 and cyclin E
Results in G1 arrest

30

Cell cycle arrest target of p53

p21

31

p53 effect on Bcl-2

Inhibition

32

ARF

Binds and sequesters MDM2

33

E2F

Hyperproliferative stress response
Active when the cell cycle is active
Activates ARF

34

Chk2, Chk1

Phosphorylate both MDM2 and p53
Prevent p53 degradation

35

Rad9

Activates Chk1

36

ATR

Genotoxic stress response
Activates in response to replication errors and single stranded DNA breaks
Phosphorylates Rad9

37

ATM

Genotoxic stress response
Activates in response to double stranded, major breaks
Activates Chk2