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CLINICAL BACTERIOLOGY > capsular staining, bacterial challenge test (disinfectants), mcfarland, ast interpretation > Flashcards

capsular staining, bacterial challenge test (disinfectants), mcfarland, ast interpretation Flashcards

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1
Q

steps in capsular staining

A
  1. Place a loopful of saline on a labelled slide.
  2. Emulsion a single colony.
  3. Place a loopful of serum and spread to make a thin smear.
  4. Air dry and heat fix gently.
  5. Cover smear with Crystal Violet. Hold over flame for a few seconds
  6. Wash excess stain with 20% Aqueous CuSO4 (Copper(II) sulfate)
  7. Blot dry and view under OIO
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2
Q

reagents used in capsular staining

A

crystal violet and 20% of aqueous copper II sulfate (CuSO4)

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3
Q

BACTERIAL CHALLENGE TEST
(Effectiveness of Disinfectants)

O means

A

no growth

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4
Q

BACTERIAL CHALLENGE TEST
(Effectiveness of Disinfectants)

+ means

A

poor growth

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5
Q

BACTERIAL CHALLENGE TEST
(Effectiveness of Disinfectants)

++ means

A

good growth

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6
Q

BACTERIAL CHALLENGE TEST
(Effectiveness of Disinfectants)

+++ means

A

excellent growthq

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7
Q

C

A

Chloramphenicol

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8
Q

CN

A

Gentamicin

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9
Q

KF

A

Cephalotin

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10
Q

AM

A

Ampicillin

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11
Q

CXM

A

Cefuroxime

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12
Q

CB

A

Carbenicillin

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13
Q

TIC

A

Ticarcillin

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14
Q

TE

A

Tetracycline

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15
Q

K

A

Kanamycin

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16
Q

Ax

A

Amoxicillin

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17
Q

CIP

A

Ciprofloxacin

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18
Q

NI/F

A

Nitrofurantoin

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19
Q

ETP

A

Ertapenem

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20
Q

SAM

A

Ampicillin-Sulbactam

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21
Q

GRAM POSITIVE ANTIBIOTICS ARE THE FOLLOWING

A

GRAM POSITIVE ANTIBIOTICS ARE THE FOLLOWING

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22
Q

E

A

Erythromycin

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23
Q

AmC

A

Amoxicillin/Clavulanic
Acid

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24
Q

P

A

Penicillin G

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25
S
Streptomycin
26
NV
Novobiocin
27
OX
Oxacillin
28
KF
Cephalotin
29
ATM
Aztreonam
30
NA
Nalidixic Acid
31
VA
Vancomycin
32
CIP
Ciprofloxacin
33
DA
Clindamycin
34
FOX
Cefoxitin
35
B
Bacitracin
36
steps in bacterial challenge test (effectiveness of disinfectants)
Procedure: 1. Place 5ml of the following disinfectants into 3 labelled tubes - Merthiolate - 75% Alcohol - Hypochlorite 2. To each tube, add 0.5 ml of bacterial suspension 3. Mix gently by tapping and start the timer 4. At the end of 30 seconds, 1 minute, and 2 minutes. Inoculate the designated area on a nutrient agar plate 5. Incubate at 35°C for 24 hours 6. Evaluate using the following: - O = no growth - + = poor growth - ++ = good growth - +++ = excellent growth
37
in the bacterial challenge test, how many Celsius is the bacteria inoculated and for how many hrs?
35 C for 24 hrs
38
0.5 McFarland standard steps
Procedure: 1. Prepare 1% Sulfuric acid (H2SO4) and 1.175% Barium chloride (BaCl2) 2. Using separate pipettes, add the amounts below
39
how many uL are there in 1 ml
1000 uL
40
chlorampenicol C, RIS
12, 17, >18
41
gentamicin CN
13, 17, >18
42
cephalotin KF
14, 17, >18
43
ampicillin AM
13, 16, >17
44
cefuroxime cxm
14, 17, > 18
45
carbenicillin cb
19, 22, >23
46
ticarcillin TICS
14, 19, > 20
47
tetracycline TE
14, 18, 19
48
kanamycin K
13, 17, >18
49
amoxycillin Ax
19, >20
50
ciprofloxacin CIP
13, 17, >18
51
nitrofurantoin NI/F
14, 16, >17
52
ertapenem ETP
12, 15, >16
53
icillin-sulbactan SAM
11, 14, >15
54
gram negative antibiotics
chloramphenicol, gentamicin, cephalotin, ampicillin, cefuroxime, ticarcillin , tetracycline, kanamycin, amoxicillin, ciprofloxacin, nitrofurantoin, ertapenem, ampicillin-sulbactan
55
erythromycin
13, 16, >17
56
amoxicillin/cluvanic acid
19, >20
57
penicillin G
28, .29
58
streptomycin
13, 17, >18
59
novobiocin
14,16, >17
60
oxacillin
17, >18
61
CEPHALOTIN
14, 17, >18
62
aztreonam
15, 21, >22
63
nalidixic acid
13, 18, >19
64
vancomycin
14, >15
65
ciprofloxaci
13, 17, >18
66
cefoxitin
13, 18, >19
67
bacitracin
8, 12, >13

CLINICAL BACTERIOLOGY (21 decks)

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