MTAP CHAP 6 - SPECIMEN COLLECTION AND PROCESSING

Question 1

4 level scheme of prioritization of specimen

specimen classified as critical beacuase they represent a potentially life-threatening illness and are from an invasive source

Answer 1

level 1

Question 2

4 level scheme of prioritization of specimen

specimens are unprotected and may quickly degrade or have overgrowth of contaminating biota

Answer 2

level 2

Question 3

4 level scheme of prioritization of specimen

specimen requires quantitation, delay iun processing may adversely affect the accuracy of quantitation

Answer 3

level 3

Question 4

4 level scheme of prioritization of specimen

are specimens that arrive in laboratory in holding or transport media

Answer 4

level 4

Question 5

4 level scheme of prioritization of specimen

specimens under level 1

Answer 5

critical/invasive

amniotic fluid
blood
brain
csf
heart valves
pericardial fluid

Question 6

4 level scheme of prioritization of specimen

unpreserved or under level 2

Answer 6

body fluids that is not under level 1
bone
drainage from wounds
feces
sputum
tissue

Question 7

4 level scheme of prioritization of specimen

quantitation required/under level 3

Answer 7

catheter tip
urine
tissue for quantitation

Question 8

4 level scheme of prioritization of specimen

preserved sample/level 4

Answer 8

feces in preservative
urine in preservative
swabs in holding medium (aerobic/anaerobic )

Question 9

when will we reject a expectorate sputum upon gram stain

Answer 9

if it reveals fewer than 25 wbc and more than 10 epithelial cells per LPO and mixed with bacterial biota

Question 10

the standard nonselective media in US

Answer 10

sheep blood agar

Question 11

stain directed to toward coloring the forms and shapes presents

Answer 11

simple stain

forms and shape

Question 12

type of stain designed to color specific components of the elements present,

Answer 12

differential stain

specific components

Question 13

type of stain directed specifically at identofocation of an organism or virus

Answer 13

antibody or DNA-probe-mediated stains

Question 14

what are the 4 stains that must be available in all diagnostic microbiology lab

Answer 14

gram stain
acid fast
calcufluor white
rapid modified wright-giema

Question 15

stain for bronchoalveolar lavage

Answer 15

wright-giemsa

Question 16

stain for tzank preparations

Answer 16

wright-giemsa

Question 17

stain for samples with complex cellular backgrounds such as bacteria, yeasts, parasites, and viral inclusions

Answer 17

wright-giemsa

Question 18

stain for flagella

Answer 18

leifson

Question 19

stain for metachromic granules of corynebacterium diphtheriae

Answer 19

methylene blue

Question 20

acid fast stain for direct smears of mycobacteria in material from the lower respiratory tract

sediments for mycobacteria (concentrated smears)

Answer 20

ziehl-neelsen

Question 21

partial acid fastness of nocardia spp

Answer 21

ziehl-neelsen spp

Question 22

acid fast stain for direct smears of mycobacteria in material from the lower respiratory tract aside from ziehl-neelsen

Answer 22

kinyoun

Question 23

acid fast stain modification of ziehl-neelsen method for cryotosporodium and cyclospora parasites

Answer 23

kinyoum

Question 24

what are the acid fast for mycobacteria in materials from the lower respiratory tract

Answer 24

ziehl neehlsen
kinyoun
fluorochrome

Question 25

acid fast stain for sediments for mycobacteria - concentrated smear, auramine, and rhodamine

Answer 25

fluorochrome

Question 26

acid fast stain for bronchoalveolar fungi and some parasitic cysts

Answer 26

calcofluor white

Question 27

routine stain for detecting microotrganism

Answer 27

traditional gram stain

Question 28

provides same differential staining but enhances red negative organisms by staining the background material green to gray-green

Answer 28

enhanced gram stain