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CLINICAL BACTERIOLOGY > IDENTIFICATION OF STAPHY > Flashcards

IDENTIFICATION OF STAPHY Flashcards

(58 cards)

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1
Q

demonstrate the presence of catalase
an enzyme that catalyzes the release of oxygen from
hydrogen peroxide (H2O2)

A

Catalase Test

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2
Q

To distinguish Staphylococci spp. And Micrococci
spp. (positive)

A

Catalase Test

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3
Q

percentage of hydrogen peroxide or H2O2 in catalase test used for the routine culture

A

3%

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4
Q

percentage of hydrogen peroxide in catalase test used for detection of catalase in an aerobe

A

15%

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5
Q

During tests bacteria protect themselves from the little effect of hydrogen peroxide which is
accumulated as an end product of aerobic
carbohydrate metabolism

A

Catalase Test

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6
Q

positive result of catalase test

A

bubble formation or effervescence

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7
Q

Best single pathogenicity test for staphylococcus

A

Coagulase (Slide or Tube Method)

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8
Q

is a biochemical test that is used
to differentiate staphylococcus aureus from other
staphylococci species like staphylococcus
epidermidis and staphylococcus saprophyticus

A

Coagulase (Slide or Tube Method)

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9
Q

a test used to detect the ability to produce the
coagulase. coagulase is an enzyme-like protein
and causes plasma to clot by converting
fibrinogen to fibrine

A

Coagulase (Slide or Tube Method)

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10
Q

2 methods of coagulase test

A

Slide Method (screening test)
Tube Method (confirmatory test)

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11
Q

specimen used in coagulase test

A

plasma (plasma, 0.5 mL rabbit’s plasma for tube)

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12
Q

among the 2 methods of coagulase test, which one detects BOUND COAGULASE (CLUMPING FACTOR)?

A

Slide Method (screening test)

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13
Q

positive results of slide method (screening test)

A

agglutination

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14
Q

among the 2 methods of coagulase test, which one detects UNBOUND COAGULASE (staphylocoagulase)?

A

Tube Method (confirmatory test)

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15
Q

incubation time of tube method coagulase test

A

2hrs, 4hrs, and another 24hrs

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16
Q

positive result of tube method coagulase test

A

coagulum/clot

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17
Q

medium used in O/F reactions

A

O/F glucose medium

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18
Q

O/F glucose medium is also called as

A

Hugh Leifson medium

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19
Q

a method for biochemical
differentiation of microbes it is used to detect the
oxidation or fermentation of carbohydrates by
bacteria.

A

Oxidation-Fermentation (O/F) Reactions

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20
Q

In O/F reactions, _____ferment glucose or
fermentative, whereas _____ fail to produce
acid under anaerobic conditions.

A

Staphylococcus , micrococci

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21
Q

sugars added in O/F reaction’s medium aside from sucrose, lactose, and glucose

A

cylos, mannitol, and maltose

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22
Q

In O/F reactions, ___
utilization of the carbohydrate will result in acid
production or color yellow in the open tube only

A

Oxidative

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23
Q

In O/F reactions, ____ utilization of the carbohydrate will
result in acid production or yellow in both open
and closed rooms

A

Fermentative

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24
Q

___ organism, meaning
absence okay it will not produce acid in either of
the tube

Study These Flashcards
A

asaccharolytic

25
what is added in hugh leifson medium to serve as a barrier for oxygen
mineral oil
26
In O/F reactions, if the closed tube turns yellow it means?
it is fermentative
27
In O/F reactions, if the open tube turns yellow it means?
oxidative
28
In O/F reactions among the genera if both tube turns yellow, is it staph or micrococci?
staph
29
In O/F reactions, if only the open tube turns yellow. Is it staph or micococci?
micro
30
active chemical component of modified oxidase test
Tetramethyl-paraphenylene diamine dihydrochloride
31
is a reagent impregnated disk recommended for use in qualitative procedures to aid in the differentiation of staphylococcus and micrococcus by the detection of the oxidase enzyme
Microdase disk
32
a positive result in modified oxidase test
blue to purple to black complex (micro lang ang psoitive sa modified OXIDASE test)
33
medium used for mannitol fermentation
Mannitol Salt Agar
34
positive result of mannitol fermentation
yellow
35
negative result of mannitol fermentation
pink
36
a yellow positive result in mannitol fermentation indicated the presence of what
staphylococcus aureusq
37
indicator used in mannitol fermentation
Phenol red
38
principle behind the mannitol fermentation
If an organism can ferment mannitol, an acidic byproduct is formed that will cause the phenol red in the agar to turn yellow. Most pathogenic staphylococci such as staphylococcus aureus will ferment mannitol and most non-pathogenic staphylococci will not ferment mannitol
39
medium used in DNAse test
DNAse Medium
40
indicator used in DNAse test
methyl green
41
what is added in DNAse test
1 N hydrochloric acid
42
positive result of DNAse test
zone of inhibition
43
a positive zone of inhibition in DNAse test identifies the presence of
staphylococcus aureus
44
a negative result in DNAse test identifies the presence of
Staphylococcus epidermidis
45
what percent of ---- is used in gelatin medium for GELATIN LIQUEFACTION/HYDROLYSIS TEST
12%
46
principle behind dnase test
If the organism that grows in the medium produces deoxyribonuclease, it breaks down dna into smaller fragments. When the dna is broken down it no longer binds to the metal grid and the green color fades and the colony is surrounded by a colorless zone.
47
Gelatin is a protein derived from the connective tissues of vertebrates that is ___
collagen
48
positive result in Gelatin Liquefaction/Hydrolysis Test
liquefaction
49
Presumptive identification of ______ is accomplished by testing for novobiocin susceptibility
staphylococcus saprophyticus
50
Staphylococcus saprophyticus is __to novobiocin whereas most other coNs are susceptible
resistant
51
___ microgram of novobiocin disk is used in novobiocin disk
5
52
STEPS IN COLLECTION OF BACTERIAL SAMPLE
1. Use sterile cotton swab to collect bacterial sample in the skin/nasal cavity 2. Inoculate the sample in MSA 3. Incubate at 37C for 18-24 hours 4. Observe for growth and color development
53
STEPS IN OXIDASE TEST
1. Using a sterile loop/applicator stick, rub a colony into the oxidase strip 2. Observe for color change (+) Purplish to black (-) No color change
54
STEPS IN CATALASE TEST
1. Place 1 drop of 3% hydrogen peroxide reagent in a glass slide 2. Emulsify a colony using applicator stick/inoculating loop 3. Observe (+) bubble formation (-) no gas bubbles
55
STEPS IN DNASE TEST
1. Using bacteria from a plate, inoculate the DNAse medium in a straight line 2. Incubate the DNAse medium for 18-24 hours 3. After incubation, add a small amount of 1N HCl to the plate, flooding the colonies. 4. Discard the excess acid and observe the media surrounding the colonies. (+) Clearing surrounding the colonies
56
STEPS IN DNASE TEST
. Using bacteria from a plate, inoculate the DNAse medium in a straight line 2. Incubate the DNAse medium for 18-24 hours 3. After incubation, add a small amount of 1N HCl to the plate, flooding the colonies. 4. Discard the excess acid and observe the media surrounding the colonies. (+) Clearing surrounding the colonies
57
STEPS IN COAGULASE TEST - SLIDE
1. Divide the slide into two sides using a marking pen. Label the left portion “T” (for test) and the right portion “C” (for control) 2. Place a drop of plasma in the T portion of the slide 3. Place a drop of saline in the C potion of the slide 4. Emulsify few colonies into the T portion 5. Emulsify few colonies into the C portion 6. Mix well for 5 seconds and observe for clumping within 10 seconds (+) clumping (-) no clumping *negative control should show no clumping
58
STEPS IN COAGULASE TEST - TUBE
1. Add 0.5 mL of plasma into a tube 2. Inoculate a loopful of bacteria 3. Incubate for 2 hours and observe for clot formation 4. If no clot is observed, incubate for another 2 hours and observe for clot formation 5. If no clot is observed after a total of 4 hours incubation, incubate further up to 18-24 hours (+) Coagulum (-) No coagulum

CLINICAL BACTERIOLOGY (23 decks)

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