CircoViradae Flashcards
(99 cards)
1
Q
Shape of circoviridae
A
Icosahedral
2
Q
Diameter of circoviridae
A
17-25nm
3
Q
Genome of circoviridae
A
Single strand
circular DNA
nucleic acid also infective (difficult to inactivate)
4
Q
how many genes does circoviridae have
A
3-4
5
Q
where does circoviridae replicate
A
in dividing cells (using polymerase enzyme)
6
Q
does circoviridae have good antigens
A
yes
7
Q
how resistant is circoviridae
A
VERY, outside can be infectious for months
8
Q
PCV1
A
non pathogenic
9
Q
PCV2
A
Responsible for PCVD (or in background)
10
Q
heat deactivation of circoviridae
A
80° for 1h
11
Q
cell type circoviridae is found in
A
immune cells
12
Q
PCV2 & PCV3
A
same disease different genome
13
Q
PCV4
A
similar to mink circovirus
14
Q
pathogenesis of Porcine circovirus
A
oronasal route (or other)
–> Rep in Immune c –> Viraemia
–> Imunosupression OR PMWS
15
Q
predisposing factors of PCVD
A
environment
16
Q
diseases connected to PCV
A
- subclinical inf. (immune sup.)
- PCV2 systemic D. (new PMWS)
- PCV2 resp D
- PCV2 enteric D
- PCV2 repro D (most imp)
- PDNS (porcine dendritic néphropathy syndrome)
17
Q
PMWS age
A
1,5 - 4 months
18
Q
PMWS symptoms
A
- diarrhea, phtisis
progressive weight loss
jaundice
rough hair
19
Q
PMWS patho
A
swollen lymph nodes (inguinal & mesenteric)
haemorrages
pneumonia
(myocardial replication)
20
Q
PDNS age
A
from 1,5 months (30-100kg)
21
Q
PDNS symptoms
A
red spots on skin
progressive weight loss
haemorrages, oedema
22
Q
PDNS patho
A
Red, enlarged LN
Glomerulonephritis
Haemo in renal cortex
23
Q
PRDC age
A
4-7 months
24
Q
PRDC symptom
A
fever
lethargy
resp
25
PRDC patho
interstitial bronchopneumonia
peribronchial fibrosis
26
Repro disorders age
pregnant
27
Repro disorders symptoms
SMEDI
28
Repro disorders pathology
fetal myocarditis (also detected in liver c & macropages)
29
can the activated immune system help the development of the disease (PCV2)
yes
30
how to control PCV2 disease
vaccine protection
31
animals susceptible of PCV2
domestic pigs and reservoir species
32
PDNS pathogenesis
Type 3 hypersensitivity
33
what happens in sows infected with PCV2 before day 70
sever lesions : abortus, mummification & embryonic death
34
what happens in sows infected with PCV2 after day 70
immune response of fetus but repro pb remains
35
Where is PRDC more frequent than PMWS
North America
36
PRDC diagnosis
- Ab resistancy
- Histological lesions of lungs
- PCV2 detected in lungs
37
how to detect PCVD
**- immunohistochemistry
- IF
- PCR**
| ELISA: for IgM in recent infections
38
Types of vaccines used for PCVD
Inactivated
Recombinant
39
what is the sow vaccine
protects from repro pb
enough protection for piglets in first few weeks
40
when is the highest uptake of the PCV virus for piglets
first few weeks of age
41
What is the piglet vaccine?
protect during and after weaning
42
which species can naturally be inf-ected with PCV
domestic pigs
wild boars
cattle
rodents (in pig farms)
43
PCV3?
similar CS, no immune complexes (no PDNS)
repro disease
| all over the world
44
PCV4 ?
role in co infections:
- PCV2
- PCV3
- PRRSV
- PEDV
- Aujesky
| china + south korea
45
what kind of infection is possible for dog Circovirus
subclinical
clinical
co-infection
asymptomatic
46
Patho of dog circovirus
pale mucosa
bloody stool
hemorrhagic in SI
splenomegaly
47
which animals can be infected by dog circovirus
dog, wolf, fox, badger
48
Avian circovirus CS
immunossupression
49
avian circovirus diseases
- Beak and feather virus
- pigeon circovirus
- goose circovirus
50
BFD which animals
young birds, below 3 years
51
BFD replication
in dividing cells (feather follicules)
| horizantal and vertical transmission
52
Acute BFD CS
- lethargy, anorexia
- resp signs
- vomit, diarrhea
| death within 2-4 weeks (secondary inf)
53
chronic BFD CS
lesions obvious after moulting, loss feather
feather and beak deformitites
| can go on for years or die within 1
## Footnote
surving birds are carriers and shedders
54
Pigeon circovirus age
1- 12 months
55
PiCV CS
Lethargy, anorexia, beak & feather deformities
| race performance decrease
56
57
Acute PiCV
similar to BFD
| death in 2-5 days
58
which cells are destroyed in PiCV
spleen, thymus and bursa
59
# Goose Circovirus
GoCV age & CS
1 week old & slow growth, feather formation
60
Chicken infectious anaemia agent
Anelloviridae/Gyrovirus
61
CAV CS
anaemia
immune suppression
62
CAV affected animals
ONLY chickens (few weeks old)
63
Epi of CAV
vertical (egg,sperm)
horizantal (direct contact, feces)
| shedding with egg up to 2 months
64
Age of CAV
below 3 weeks: disease/severe
over 3 weeks: mild
65
pathogenesis of CAV
PO > intestine (resp) > viraemia > dividing cells
66
mainly in which cells does CAV infect?
T cells
67
how can we differentiate bursitis and CAV
mainly B cells in bursitis
68
clinical CAV
- Anaemia-dermatitis
- 10-14 day old
- rare
| vertical transmission
69
Subclinical CAV
- resistance with age
- frequent
- decrease productivity
| horizantal transmission
70
CAV clinical signs & age
up to 1 month
incubation 7-10 days
not typical clinical signs
71
CAV pathology
haemorrhages, in gizzard and proventriculus
thinner blood
anemia
72
histo path of CAV
Myeloid and lymphoid cells destroy
73
diagnosis of CAV
virus isolation on lymphoid cells
IF, PCR
74
DD of CAV
| (Diferential diagnosis)
- **infectious bursitis**
- newcastle disease
- avian influenza
- viral inclusion body hepatitis
75
Vaccination of hens against CAV
12-15 wo
76
CAV vaccine
in young: inactivated virulent
live, moderately attenuated
| by drinking
77
papillomaviridae diameter
40-55nm
78
papillomaviridae shape
icosahedral
79
papillomaviridae resistance
VERY, inf. for month in evironment
80
papillomaviridae genome
double stranded, linear DNA
| different than PCV & CAV
81
can papillomaviridae replicate in tissue culture
no, needs keratin
82
# T/F
papillomaviridae uses viral transcriptase
True
83
# T/F
papillomaviridae is stenoxen but not oncogene
F, stenoxen and oncogene
84
84
Bovine papillomatosis types
6
85
BPM that infects horses
1,2
86
BPV in the skin all over the body
1,2,3
87
BPV in the intestines
4
88
BPV in skin of udder
5,6
89
where can be found infected virion of BPV
stratum corneum
| virion assembly in stratum granulosum
90
Incubation, spreading & intake of BPV
- 1-2month in young animals
- direct contact, fly vector (all over the world)
- trauma, errosion (skin, mucosa)
91
Clinical signs of BPV
nodules: near eye, horn base, abdomen, neck, dug, withers
| rare: oesophagus, oral cavity
92
93
BPV treatment
surgical removal, if irritating
| not AUTOVACCINES bc food producing animals
94
Horse papillomavirus
EPV 2 types
on head & neck
iatrogenic spreading
| under 3 years old
95
Horse sarcoid cause
BPaV 1&2
head, abdomen & hoof
agressive but no metastasis
| horse cannot spread virus (rep cycle is stopped)
96
Dog /Cat papillomavirus can be found where
oral cavity, genitalia
97
Dog /Cat papillomavirus treatment
autovaccine
98
