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Flashcards in CItric Acid Cycle Deck (48)
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1

products of conversion of pyruvate to acetyl CoA?

2 NADH are formed

2

where does the conversion of pryuvate into acetyl COA

inside the mitocondria

3

WHERE DOES THE CITRIC ACID CYCLE OCCUR? WHERE DOES OXIDATION PHOSPHORYLATION OCCUR?

BOTH INSIDE THE MITOCONDRIA

4

eukaryotic cells without mitocondria

mature erhytocytes

5

3 examples of cells with high energy needs that have a lot of mitocondria as a result 

heart for contraction

kidney for transport

liver for biosynthesis

6

 In ____ cells, mitochondria account for up to 20% of the total protein.

liver

7

The citric acid cycle occurs in the ___ of the mitocondria

matrix

8

where are oxidative phosphorylation enzymes? 

embedded in the inner membrane facing the matrix.

9

 traffic between the cytoplasm and matrix space is controlled by specific transport systems, except for

 O2 and CO, which diffuse through

10

functions of the citric acid cycle (3)

A. Converts a number of different fuels to a common mobile fuel (NADH).
B. Serves as the final meeting place of nearly all oxidizable substrates.
C. Provides intermediates for biosynthesis.

11

 Oxidation of Pyruvate to Acetyl-CoA

 

first step? what is the prosthetic group?

 Condensation and decarboxylation by pyruvate dehydrogenase (E1)

prosthetic group -- TPP (thiamine b1)

12

 Oxidation of Pyruvate to Acetyl-CoA

 

second step? what is the prosthetic group?

Oxidative transfer by dihydrolipoyl transacetylase

prosthetic group -- lipoamide

 

13

 Oxidation of Pyruvate to Acetyl-CoA

 

third step? what is the prosthetic group?

Transacetylation by dihydrolipoyl transacetylase

 

prosthetic group -- lipoamide

14

 Oxidation of Pyruvate to Acetyl-CoA

 

fourth step? what is the prosthetic group?

Oxidation of dihydrolipoamide by dihydrolipoyl dehydrogenase

Prosthetic group: FAD

15

The pyruvate dehydrogenase complex has multiple 

Multiple Lipoamides/E2

16

In the pyruvate dehydrogenase complex

Dihydrolipoamide

______ is the site of action of arsenite poisoning

17

Importance of Lipoamide as a Swinging Arm in the pyruvate dehydrogenase complex: 

Kinetics are fast

18

___ groups are not going on and off an ezyme, are always there

prosthetic

19

Acetyl CoA + oxaloacetate + H2O --->  ____

by citrate synthetase

 citrate + CoA + H+

20

important feature of this step?

The hydrolysis step has a ΔG°ʹ′ = -7.5 kcal/mol. Wasteful?

21

Acetyl CoA + oxaloacetate + H2O → citrate + CoA + H+

enzyme? 

Citrate synthetase

22

Acetyl CoA + oxaloacetate + H2O → citrate + CoA + H+

rxn type?

condensation/hydrolysis

23

Citrate --->

with aconitase

cis-aconitate + H2O

24

 Citrate  cis-aconitate + H2O 

enzyme?

Aconitase

25

Citrate ----> cis-aconitate + H2O

rxn type>??

dehydration

26

cis-Aconitate + H2O ---> 

with aconitase

isocitrate

27

 cis-Aconitate + H2O --->  isocitrate

rxn type?

 

hydration

28

 cis-Aconitate + H2O  ---> isocitrate

eznyme?

acotinase

29

α-Ketoglutarate + NAD+ + CoA ----> 

α-Ketoglutarate dehydrogenase complex

succinyl CoA + CO2 + NADH

30

α-Ketoglutarate + NAD+ + CoA --> succinyl CoA + CO2 + NADH

enzyme?

α-Ketoglutarate dehydrogenase complex