Crossword CPA #7

Question 1

period in which the maximum number of microbes are growing in a medium

Answer 1

stationary

Question 2

type of media; aka synthetic media

Answer 2

defined

Question 3

organism that prefers a pH less than 6.5

Answer 3

acidophiles

Question 4

type of method to count cells; ie microscopic counts, serial dilution with variable plate counts

Answer 4

direct

Question 5

how bacteria reproduce; population doubles every generation time

Answer 5

binary fission

Question 6

organisms that prefer to grow in salty areas

Answer 6

halophiles

Question 7

sticky formation that causes tooth decay

Answer 7

biofilm

Question 8

organism that gets energy from chemicals

Answer 8

chemotroph

Question 9

type of method to count cells; ie turbidity, metabolic measurements, dry weight, PCR

Answer 9

indirect

Question 10

term used to graphically describe bacteria growth (2 terms)

Answer 10

exponential or logarithmic

Question 11

organism that causes dental caries

Answer 11

Streptococcus mutans

Question 12

process to preserve cells by removing all liquid in the broth so that you can deep-freeze the pellet

Answer 12

lyophilization

Question 13

period of bacteria growth

Answer 13

logphase

Question 14

ie a nutrient rich test tube or plate that allows bacteria to grow

Answer 14

medium

Question 15

oxygen is deadly to these organisms

Answer 15

obligate anaerobes

Question 16

organisms that prefer a pH greater than 7.5

Answer 16

alkalinophiles

Question 17

ie the small amount of bacteria that you put in a nutrient rich test tube/plate to grow

Answer 17

inoculum

Question 18

organisms that prefer to grow in very, very hot temperatures (80C+)

Answer 18

hyperthermophiles

Question 19

how long it takes for a particular bacteria population to double

Answer 19

generation time

Question 20

organisms that prefer a pH of 6.5 to 7.5

Answer 20

neutrophiles

Question 21

refers to either a test tube full of microbes or the process of inoculating a test tube to allow microbes to grow

Answer 21

culture

Question 22

ie spots of bacteria on a plate

Answer 22

colonies

Question 23

organisms that need 2-10% of oxygen to survive (ie H. pylori)

Answer 23

microaerophiles

Question 24

organisms that prefer to grow in cold temp. (below 15C)

Answer 24

psychrophiles

Question 25

organisms that prefer to grow in hot, but not too hot, temps (45-80C)

Answer 25

thermophiles

Question 26

growth-limiting nutrient for many microbes; found in amino acids

Answer 26

nitrogen

Question 27

organisms that can survive with or without oxygen, but grow faster with O2 (ie E. coli)

Answer 27

facultative anaerobes

Question 28

organisms that get their carbon from other organsims

Answer 28

heterotrophs

Question 29

test tube with agar solidified at an angle to allow for more agar surface are to grow microbes

Answer 29

slant

Question 30

period in which bacteria death exceeds new bacteria formation in a medium

Answer 30

deathphase

Question 31

method bacteria use to estimate their population size in an area

Answer 31

quorum sensing

Question 32

organisms that need O2 to survive

Answer 32

obligate aerobes

Question 33

a single one of these is required for a pure culture; appears as one spot on agar plate

Answer 33

colony forming unit (CFU)

Question 34

organisms that prefer to grow when the temperature is 20C to 40C

Answer 34

mesophiles

Question 35

type of media; helps you pick out one particular type of bacteria if 2 different bacteria are growing on your plate due to visible differences in the media

Answer 35

differential

Question 36

ie a broth that contains only the progeny of one bacterium cell

Answer 36

pure culture

Question 37

organisms that can detoxify O2 to survive on O2-filled environments

Answer 37

aerotolerant anaerobes

Question 38

would not be carbon, hydrogen, oxygen, or nitrogen

Answer 38

trace elements

Question 39

a method in which cells of interest in a population of different cells are tagged with florescent dyes and then a machine is used to count each tagged cell

Answer 39

flowcytometry

Question 40

type of media; allows for several different types of microbes to possibly grow

Answer 40

complex

Question 41

chemicals needed to live; must be obtained from diet because you can't make them in your body

Answer 41

growth factors

Question 42

type of media; encourages your preferred bacteria to grow and not others

Answer 42

selective

Question 43

period of slow growth after inoculation

Answer 43

lagphase

Question 44

how cloudy your cultured media broth is

Answer 44

turbidity

Question 45

organisms that make their own food with carbon

Answer 45

autotroph

Question 46

organisms that get their energy from sunlight

Answer 46

phototroph

Question 47

liquid media

Answer 47

broth