DNA structure, phases of DNA replication -- Lecture 5 Flashcards

1
Q

nucleotides consist of:

A

5-C sugar
phosphate group
nitrogenous base

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2
Q

the phosphate group is at the ___ end of the nucleotide

A

5’ end

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3
Q

the ___ is at the 5’ end of the nucleotide

A

phosphate group

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4
Q

the phosphate group is at the 5’ end of the ___

A

nucleotide

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5
Q

the OH is at the ___ of the nucleotide

A

3’

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6
Q

the ___ is at the 3’ end of the nucleotide

A

OH

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7
Q

the OH is at the 3’ end of the ___

A

nucleotide

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8
Q

if the OH is at the 2’ end of the nucleotide…

A

nucleotide is RNA

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9
Q

if the H is at the 2’ end of the nucleotide

A

nucleotide is DNA

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10
Q

phosphodiester bond formation:

DNA is only synthesized in the ___ direction by DNA polymerase enzymes

A

5’ to 3’ direction

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11
Q

phosphodiester bond formation:

DNA is only ___ in the 5’ to 3’ direction by DNA polymerase enzymes

A

synthesized

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12
Q

phosphodiester bond formation:

DNA is only synthesized in the 5’ to 3’ direction by ___

A

DNA polymerase enzymes

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13
Q

phosphodiester bond formation:

___ is only synthesized in the 5’ to 3’ direction by DNA polymerase enzymes

A

DNA

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14
Q

DNA structure:

DNA backbone is held together by covalent ___

A

phosphodiester bonds

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15
Q

DNA structure:

DNA backbone is held together by ___ phosphodiester bonds

A

covalent

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16
Q

DNA structure:

___ is held together by covalent phosphodiester bonds

A

DNA backbone

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17
Q

DNA structure:

nitrogenous bases of complimentary DNA strands are held together by ___

A

H-bonds

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18
Q

DNA structure:

1 complete turn of DNA has ___

A

10 base pairs

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19
Q

DNA structure:

the ___ has a free phosphate group attached to the 5’ carbon

A

5’ end

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20
Q

DNA structure:

the 5’ end has a ___ attached to the 5’ carbon

A

free phosphate group

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21
Q

DNA structure:

the 5’ end has a free phosphate group attached to the ___

A

5’ carbon

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22
Q

DNA structure:

the ___ has a free hydroxyl (-OH) group at the 3’-carbon

A

3’ end

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23
Q

DNA structure:

the 3’ end has a ___ at the 3’-carbon

A

free hydroxyl (-OH) group

24
Q

DNA structure:

the 3’ end has a free hydroxyl (-OH) group at the ___

A

3’-carbon

25
DNA structure: antiparallel
DNA strands are in opposite orientations
26
phases of DNA replications: DNA replication has 3 phases:
initiation elongation termination
27
phases of DNA replications: initiation (what is it)
starting of the process bringing together all the necessary enzymes to replicate DNA
28
phases of DNA replications: elongation (what is it)
actual process of replicating the DNA
29
phases of DNA replications: termination (what is it)
make sure the process is appropriately stopped
30
semiconservative DNA replication: step 1:
old DNA strands are separated H-bonds are broken
31
semiconservative DNA replication: step 2:
each old DNA strand is used as a template for a new DNA strand (Semiconservative Replication)
32
semiconservative DNA replication: step 3:
the new DNA molecules are 1/2 old strand and 1/2 new strand
33
initiating DNA replication: replication complex (RC) -- what is it
large complex containing the enzymes needed to replicate DNA, interacts w/ the DNA
34
initiating DNA replication: where do RCs bind to the DNA to begin replication?
origin of replication
35
initiating DNA replication: origin of replication (ori) -- what is it
sequences of DNA where replication begins
36
initiating DNA replication: why do eukaryotic chromosomes have thousands of oris?
eukaryotic chromosomes are very big multiple oris allow for a much faster replication process
37
chromosomes get copied beginning at several ori's ___
simultaneously
38
chromosomes get ___ beginning at several ori's simultaneously
copied
39
___ get copied beginning at several ori's simultaneously
chromosomes
40
chromosomes get copied beginning at several ori's simultaneously: replication complexes bind to ___ this forms
oris replication bubbles as replication forks move out in opposite directions
41
chromosomes get copied beginning at several ori's simultaneously: (steps)
replication complexes bind to the ori's DNA strands are separated DNA replication proceeds bidirectionally
42
direction of replication: DNA strands are separated at replication forks by ___
helicase
43
direction of replication: DNA strands are ___ at replication forks by helicase
separated
44
direction of replication: DNA strands are separated at ___ by helicase
replication forks
45
direction of replication: ___ are separated at replication forks by helicase
DNA strands
46
direction of replication: helicase (what does it do)
breaks the H-bonds b/n strands
47
direction of replication: on the ___: new DNA is synthesized continuously in the 5' --> 3' direction by DNA Pol III
leading strand
48
direction of replication: on the leading strand: new DNA is synthesized continuously in the ___ direction by DNA Pol III
5' --> 3'
49
direction of replication: on the leading strand: new DNA is synthesized continuously in the 5' --> 3' direction by ___
DNA Pol III
50
direction of replication: on the leading strand: new DNA is ___ in the 5' --> 3' direction by DNA Pol III
synthesized continuously
51
direction of replication: on the lagging strand: new DNA is synthesized in discontinuous fragments in the 5' --> 3' direction (occurs more ___)
slowly
52
direction of replication: on the lagging strand: new DNA is synthesized in discontinuous fragments in the ___ direction (occurs more slowly)
5' --> 3'
53
direction of replication: on the lagging strand: new DNA is synthesized in ___ in the 5' --> 3' direction (occurs more slowly)
discontinuous fragments
54
direction of replication: on the lagging strand: new DNA is ___ in discontinuous fragments in the 5' --> 3' direction (occurs more slowly)
synthesized
55
direction of replication: on the lagging strand: ___ is synthesized in discontinuous fragments in the 5' --> 3' direction (occurs more slowly)
new DNA
56
direction of replication: on the ___: new DNA is synthesized in discontinuous fragments in the 5' --> 3' direction (occurs more slowly)
lagging strand