Enzyme classifications Flashcards

(37 cards)

1
Q

What are the 6 classifications of Enzymes

A

oxidoreductases
transferases
hydrolases
lysases
ligases
isomerases

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2
Q

What is the function of oxidoreductases

A

Electron transfer in REDOX

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3
Q

What is an example of an oxidoreductase enzyme

A

Cytochrome P450

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4
Q

Where is Cytochrome P450 found and it’s function

A

in liver and detoxifies drugs

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5
Q

What are CYP450 inhibited by and what is it found in

A

flavanols in grapefruit

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6
Q

What are CYP450 induced by

A

alcohol

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7
Q

What’s the role of a transferase

A

To transfer functional groups (phosphate)

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8
Q

An example of a transferase

A

Kinases

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9
Q

What’s the role of kinase

A

to transfer phosphate from ATP to proteins

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10
Q

What is kinase crucial for

A

cell signalling ( immune response)

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11
Q

What are overactive kinase associated with

A

Cancer

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12
Q

What is the role of hydrolases

A

To break bonds using water

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13
Q

An example of hydrolase

A

proteases

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14
Q

What is the role of proteases

A

break peptide bonds

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15
Q

What are the 2 types of sites

A

catalytic and pH

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16
Q

What are proteases involved in

17
Q

What are AAA proteins

A

amino acid analysis

18
Q

What are AAA protiens specialised for

A

controlled protein degradation

19
Q

What is the role of lysases

A

Break/form bonds without hydrolysis or oxidation

20
Q

An example of lysases

21
Q

Role of dehydratase

A

removing water to create double bonds

22
Q

What is the role of isomerases

A

rearrangement within molecules

23
Q

An example of isomerases

A

Topoisomerases

24
Q

What’s the role of topoisomerases

A

To untangle DNA

25
What are the 2 types of topoisomerase
type1- single stranded type 2 - double strand
26
Does type one require ATP
NO
27
Does type 2 require ATP
yes
28
What is the role of ligases
join molecules using ATP
29
An example of ligases
DNA ligase
30
Role of DNA ligase
repair DNA breaks essential in cloning, PCR and biotech
31
What are the 4 regulation techniques of enzyme activity
Expression compartmentalisiation Feedback control Degradation
32
What is expression controlled by
Transcription and translation
33
How is expression regulated
transcription factors binding to DNA to turn genes on or off
34
How is compartmentalisation regulated
enzymes localised within organelles preventing unwanted reactions
35
How is positive feedback regulated
stimulates further activity
36
How is negative feedback regulated
inhibits via allosteric inhibition
37
How is degradation regulated
enzymes limited lifespan ubiquitin destructs enzyme and proteosome breaks proteins-prevents over-activity and maintains homeostasis