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Flashcards in Escherichia Deck (56):
1

What is the single most common facultative anaerobic gram negative bacteria that is part of the normal flora in the GI tract of animals 

Escherichia Coli 

2

E. Coli is motile with peritrichous _____ and _____

flagella and fimbriate 

3

What pathogenesis does E. coli cause in almost every species it infects?

enteritis and septicemia 

4

What pathogenesis does E. coli cause in pigs?

edema disease 

5

True/False: E. coli causes an opportunistic infections of canine pyometra and cystitis as well as ruminant mastitis

True

*Staph aureus, Strep agalatia, and E coli are the causitive agents of mastitis

 

side note (Klebsiella, nocardia, truperella, and listeria are also possible causative agents of mastitis)

6

It is difficult to make vaccines against E. coli because the ___ antigens, that are part of the gram negative cell wall, create a lot of variability 

O antigens

7

______ is released from the E. coli bacteria when they die and can lead to fever and septic shock in the animals 

Lipid A  

 

8

E. coli can have capsules (____ antigen), flagella (____antigen) and adhesins (___antigen)

capsule- K antigen

Flagella- H antigen

Adhesins- F or P antigen 

 

*lots of different serotypes due to these possible antigens

9

finbria and pili are the adhesins that promote adherence to ____ on the epithelial cells of the intestinal tract and other target cells 

glycoproteins 

10

What is the most important immune component against E. coli?

The normal flora of the GI tract

*extremely important to avoid binding of these types of bacteria 

11

_____(ETEC) is an E. coli adhesion that causes enteric disease and releases a toxin. This is for adhesion in the _____

enterotoxogenic, small intestine 

12

_____(EIEC) mediates attach to the _____ cells to penetrate and cause dissemination throughout the body. 

enteroinvasive, small intestine 

13

_____(EAEC) forms a biofilm around the lumen of the _____ and does not allow for normal absorption and secretion, leading to diarrhea 

enteroaggregative, small intestine 

14

_____(EPEC) binds to the _____ in order to highjack the enterocyte and completely destroy the microvilli causing attaching and effacing lesions 

enteropathogenic, small intestine 

15

Enteropathogenic adhesions in the small intestine has a ____ forming pilus

bundle 

Bfp

16

_____(EHEC)  is an adhesion that utilized outer membrane protein A to bind to the _____. Causing attaching and effacing lesions

Enterohemorrhagic, large intestine 

17

The enterohemorrhagic adhesions has ____membrane proteins 

outer 

OmpA 

18

Enterohemorrhagic E. coli secretes what toxin?

Shiga-like toxin 

19

Enterotoxins results in deregulation of water and electrolyte secretion resulting in ____ in neonates

diarrhea 

20

How many toxins are produced by the enterotoxic E.coli?

three

21

_____ toxin binds to the endothelial cells and causes protein synthesis inhibition and death of the endothelial cells resulting in bloody diarrhea 

Shiga-like toxin 

22

Shiga like toxin inhibits ____

protein synthesis

23

Enteroinvasive E. coli produces a _____ necrotizing factor (CNF) that mediates the uptake of the bacteria by epithelial cells so they can disseminate 

cytotoxic 

24

______ encoded toxin is present in many different kinds of E. coli and cause damage to endothelial cells and results in diarrhea 

Plasmid 

25

Enterohemolysis is present in ______ E. coli 

enterohemorrhagic 

26

_______ is a secretion system with needle like structures that come out of the cell wall of the bacteria and secrete effector proteins into the extracellular environment  or into host cells. 

Type III secretion system 

27

True/False: E. coli are part of the normal flora of the GI tract

True 

28

How is E. coli transmitted? 

fecal oral route 

29

Most strains of E. coli do not cause disease, however they can _____virulence factors via transduction, conjugation, and transformation 

Acquire 

30

Enterotoxigenic diarrhea is present more in ____

neonates 

31

If you visit a swine farm where there is a report of an outbreak on neonate pigs of watery diarrhea. You isolate a gram negative organism. What is at the top of your differentials?

Enterotoxogenic E. coli 

32

In Enterotoxigenic Diarrhea, the E. Coli needs to produce both ____ and _____

adhesins and enterotoxins 

*needs to adhere to enterocytes of the small intestine

33

Enterotoxigenic E. coli leads to ____diarrhea 

watery 

34

True/False: Large bowel cells are susceptible to the enterotoxigenic toxin

False

LARGE BOWEL CELLS ARE NOT SUSCEPTIBLE

35

True/False: Enterotoxigenic diarrhea leads to high mortality unless fluid and elecrolyte imbalance is corrected 

True 

36

Enteropathogenic E coli causes ____ and _____lesions 

attaching and effacing lesions in the GI tract 

 

*hallmark of the disease 

37

_____ is responsible for intimate attachments via Tir with enteropathogenic E. coli

intimin 

38

Once Enteropathogenic E. coli binds to the enterocytes that utilize _____systems that produce effacing lesions and diarrhea 

Type III secretion system 

39

_____ brings the bacteria very close to the enterocyte, which causes the collapse of the microvilli 

intimin 

40

Enterohemorragic E. coli causes attaching and effacing lesions in the _____ intestine 

large 

41

What is the main virulence factor of Enterohemorragic E. coli?

Shiga- like toxin 

42

Enterohemorragic E. coli causes ____ diarrhea 

hemorrhagic (bloody)

43

Enterohemorragic E. coli causes _____ in humans as a result of the shiga-like toxin 

hemolytic uremic syndrome 

44

Enterohemorragic E. coli has ____ potential. Need to make sure to cook your meat!

zoonotic 

45

Enteroinvasive E. coli can be caused in neonates due to inadequate _____ 

colostrum 

46

_______ E. coli posses CNF-1 that is very important virulence factor. CNF induces the bacterial uptake into the host cell so that it can disseminate 

Enteroinvasive E. coli 

47

The _____ is a very important virulence factor for Enteroinvasive E. coli so that it can escape phagocytosis and complement mediated lysis 

capsule 

48

_____ disease is often a fatal enterotoxemia of weaned pigs 

Edema 

49

What is the causative agent of edema disease in pigs?

enterohemorrhagic E. coli 

*causes important mortality events in swine and effects the swine industry 

A image thumb
50

What is the most important virulence factor in edema disease?

Shiga-like toxin 

 

*extensive generalized edema 

51

_____ of fowl is a widespread disease in birds that can cause embryonic death and is economically important for the poultry industry 

colibacillosis 

52

True/False: it is imperative that the dam be exposed to microorganisms before partuition for antibodies to be made for secretion into colostrum 

True 

53

You are able to grow E. coli in Blood agar and Maconkey agar. Ecoli is lactose ____ in Maconkey agar 

positive

*will look purple 

54

What does E. coli look like under the microscope 

Gram negative rods with rounded ends 

A image thumb
55

What agar is made specificaly for identification of E. coli?

Eosin Methylene blue agar 

*will have green metallic sheen when positive 

56

True/False: Fluid and elecrolyte therapy is a good treatment for E.coli 

True