E. coli is a gram ______ bacteria.
True/False. E.Coli is part of the normal flora of animals.
How is strain identification of E. coli commonly done?
Serotyping (ID of bacterial O:K:F:H antigens) and virotyping (ID by specific toxic activity, virulence factor or virulence gene)
What are the antigens of E. coli?
O - LPS
K - capsule
H - flagella
F or P - adhesins
What are the clincal signs for enterotoxigenic E. coli?
Diarrhea is water and non-bloody
Minimal inflammatory changes
Which E. coli causes edema disease in weaned pigs?
Shigatoxigenic E. coli
Which species/age group does enterotoxigenic E. coli affect?
Neonatal and weaned pigs
Neonatal calves and lambs
Dogs and horses
How is E. coli typically transmitted?
What is the virulence factor for enteropathogenic E. coli?
Bundle forming pilus
What protein is used for attachment by enterpathogenic E. coli? What is the effect of the attachment?
Initimin is an E. coli protein for attachment
Causes the collapse of microvilli in distal small intesting and upper large intestine
What are the clinical sings of enteropathogenic E. coli?
Watery diarrhea in all animals
No blood or leukocytes
Which type of E. coli is the causative agent for neonatal colibacillosis and colisepticemia in pigs?
Enterotoxigenic E. coli
Which type of E. coli has zoonotic potential?
Enterohemorrhagic E. coli
What does E. coli cause in dogs?
Colisepticemia in neonatal pups
Pyometra in bitches
What does E. coli cause in poultry?
What is the disease caused by E. coli that is a peracute condition that results in death due to endotoxemia?
How do you diagnose enteric disease caused by E. coli?
Fecal sample for culture
What test would you use to test for Lipid A?
Limulus Amebocyte (LAL) Test
Lipid A is the endotoxin of gram negative bacteria
What is the benefit of having the family of enterobacteriaceae as part of the normal flora in the GI tract?
Microbial antagonism - protect GI tract against foreign bacteria
Breakdown and adsorption of the food
Vitamin K production
What are the most prevalent bacteria in nosocomial infections?
Infections acquired from the hospital
Which enterotoxins of E. coli cause diarrhea?
They are heat liable and stable, both plasmid encoded
What are the virulence factors of necrotoxigenic E. coli (NETC) and what does it cause?
Cytotoxic necrtizing factors 1 and 2 (cause necrosis of cells...obvii)
Causes UTI infections (or so they think, "they" aren't really sure)
Which rough strain is used to make a vaccine for coliform mastitis?
J5 strain. Works on other bacteria.