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Flashcards in Escherichia coli - Exam 2 Deck (23):
1

E. coli is a gram ______ bacteria.

Negative

2

True/False. E.Coli is part of the normal flora of animals.

True

3

How is strain identification of E. coli commonly done?

Serotyping (ID of bacterial O:K:F:H antigens) and virotyping (ID by specific toxic activity, virulence factor or virulence gene)

4

What are the antigens of E. coli?

O - LPS

K - capsule

H - flagella

F or P - adhesins

5

What are the clincal signs for enterotoxigenic E. coli?

Diarrhea is water and non-bloody

Minimal inflammatory changes

6

Which E. coli causes edema disease in weaned pigs?

Shigatoxigenic E. coli

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7

Which species/age group does enterotoxigenic E. coli affect?

Neonatal and weaned pigs

Neonatal calves and lambs

Dogs and horses

8

How is E. coli typically transmitted?

Fecal-oral route

9

What is the virulence factor for enteropathogenic E. coli?

Bundle forming pilus

10

What protein is used for attachment by enterpathogenic E. coli? What is the effect of the attachment?

Initimin is an E. coli protein for attachment

Causes the collapse of microvilli in distal small intesting and upper large intestine

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11

What are the clinical sings of enteropathogenic E. coli?

Watery diarrhea in all animals

No blood or leukocytes

12

Which type of E. coli is the causative agent for neonatal colibacillosis and colisepticemia in pigs?

Enterotoxigenic E. coli

13

Which type of E. coli has zoonotic potential?

Enterohemorrhagic E. coli

14

What does E. coli cause in dogs?

Colisepticemia in neonatal pups

Pyometra in bitches

Lower UTIs

15

What does E. coli cause in poultry?

Omphalitis 

Colisepticemia

Coligranuloma

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16

What is the disease caused by E. coli that is a peracute condition that results in death due to endotoxemia?

Coliform mastitis

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17

How do you diagnose enteric disease caused by E. coli?

Fecal sample for culture

18

What test would you use to test for Lipid A?

Limulus Amebocyte (LAL) Test

Lipid A is the endotoxin of gram negative bacteria

19

What is the benefit of having the family of enterobacteriaceae as part of the normal flora in the GI tract?

Microbial antagonism - protect GI tract against foreign bacteria

Breakdown and adsorption of the food

Waste processing

Vitamin K production 

20

What are the most prevalent bacteria in nosocomial infections?

Infections acquired from the hospital

Klebsiella pneumonia

Klebsiella oxytoca

Enterobacter cloacae

Proteus mirabilis

Citrobacter freundii

21

Which enterotoxins of E. coli cause diarrhea?

AB toxins

They are heat liable and stable, both plasmid encoded

22

What are the virulence factors of necrotoxigenic E. coli (NETC) and what does it cause? 

Cytotoxic necrtizing factors 1 and 2 (cause necrosis of cells...obvii)

Causes UTI infections (or so they think, "they" aren't really sure)

23

Which rough strain is used to make a vaccine for coliform mastitis?

J5 strain. Works on other bacteria.