Exam 1 Flashcards

(54 cards)

1
Q

What are the two forms of Glycocalyces?

A

1) Hard hydrophobic
Capsule
2) Sticky hydrophilic

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Discuss the hard hydrophobic capsule

A

Tightly attached to the exterior cell wall or membrane
Hydrophobic
Inhibits recognition
Mimics what looks normal in the host

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Discuss the sticky hydrophilic form

A

Slime layer
Hydrophilic
Loose and water soluble
Sticky (viscous)
Attachment function
Produces a biofilm
Allows a bunch of cells to stick together to cause more damage
Plague in teeth

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

What does glycocalyes mean?

A

Sugar cup
It’s made of Polysaccharides
Polypeptides

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What are the two jobs of the glycococalyex?

A

1) Protection against desiccation
Removing water
Allows the bacteria to mimic the host
2) Virulence factor
Anything that is produced or secreted from a pathogen that can lead to disease
Can be components of the pathogen e.g. components of cell wall, adhesion molecules, enzymes/proteases (enzymes that break down proteins), and toxins ( endotoxins and exotoxins)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

In prokaryotes, how does a flagella operate?

A

It whips like a propellor and is reversible

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

In eukaryotes, how does the flagella operate?

A

It whips back and forth and creates forward motion

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

In bacteria, the flagella is _____ and _______

A

Thicker; hallow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

In archaea, the flagella is ______ and ______

A

Thinner, and not hallow

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Which eukaryotic cells have flagella?

A

Algae, protozoan, and sperm cells in mammals

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

What are the arrangements of flagella?

A

Monotrichous, Lophotrichous,
Amphirichous
Peritrichous

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Explain Monotrichous

A

Polar, single flagellum at one end

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Explain Lophotrichous

A

Many flagella at a polar end

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Explain Amphirichous

A

More than one flagellum at both end

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Explain Peritrichous

A

Many flagella
Covers surface

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Explain the flagella in spirochetes

A

Two flagella spiral tightly around cell body
Endoflagella- between cell membrane and a second outer membrane
Forms an axial filament

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

How does a spirochete move?

A

In a corkscrew movement

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Examples of spirochetes?

A

Syphilis and Lyme Disease

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Explain the movement of cells with flagella.

A

Movement is in response to the environment
Movement in response to stimulus= taxis
Light stimulus- phototaxis
Chemical stimulus- chemotaxis
Type of stimulus= direction of movement
Moving toward a stimulus- positive taxis
Moving away from a stimulus= negative taxis
Moving towards in a low concentration to a high concentration
Moving away from a high concentration to a low concentration
Runs and tumbles
Runs are involved in more positive response either towards or a away
Tumbles are changing direction
These are all random
What they are sensing this that change in concentration
The further away the more tumbles vs run
The closer, the more runs vs. tumbles

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

What is a Fimbriae?

A

Sticky, proteinaceous projections
Hundred per cell
Shorter than flagella
Used to adhere
Other bacteria, hosts, environment
Important function in biofilms
Organized bacterial communities

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

What is a pilus?

A

Long hallow tubes
Composed of pilin
Modified fimbriae

Longer than fimbriae, shooter than flagella
One ore two per cell

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

What is the function of a pilus?

A

Involved in conjugation

23
Q

Explain conjugation

A

Joining of two bacterial cells to transfer DNA from one ell to another
Conjugation pili or sex pili
Not really sexual, but it is a form of genetic diversity

24
Q

What are bacteria cell walls composed of?

A

peptidoglycan

25
What makes up peptidoglycan?
sugars and peptides
26
What are the functions of the cell wall?
Provides structure and shape Protects from osmotic forces Assists some cells in attachment Excludes some antimicrobial drugs
27
Define Efficacy
Targeting or having an effect on the pathogen with no to little effect on the host.
28
True or False: The cell wall of archaea is made of peptidoglycan?
False
29
Who came up with the Gram staining technique?
Hans Christian Gram
30
Crystal violet stain....
Purple, Gram + bacteria
31
Saffrin stain...
Purple, Gram -
32
Explain why the stain adheres to Gram + bacteria.
Thick cell wall Peptidoglycan Lipoteichoic acids- anchor cell wall to cell membrane Beams that hold cell wall on to the membrane When we stain a Gram + cell it will turn purple Thicker cell wall retains this longer because they have a thicker cell wall
33
Explain why the Saffrin stain works with Gram -
Thin cell wall Peptidoglycan between two lipid bilayers of membranes Inner membrane and outer membrane Composted of lipopolysaccharide (LPS) Unique to Gram - and how they cause disease
34
What is the purpose of the Bilayer membrane in Gram - bacterian?
Outer membrane inhibits disease treatment Resists penicillin access to peptidoglycan Penicillin messes with the amphipathic nature of the cell wall and the membrane
35
Explain LPS:
Lipid and sugar Endotoxin Specifically the Lipid A Released from dead cells when cell wall disintegrates Fever, vasodilation, inflammation, and blood clotting, and endotoxin shock The above symptoms need to be monitored for after antibiotics are given as Lipid A is released as the cell die
36
What are Acid-fast bacteria?
A microbacteria such as leprosy and tuberculosis. Cell walls are different Looks similar to Gram + as it is thick like a Gram + Contains 60% mycolic acid; very waxy A different stain is needed because the Gram test is water based Mycolic acid is hydrophobic If you try to stain with Gram stain will be repelled and will not make it into the cells As such they are resistant to dessication Cause acid-fast to be very hardy Can survive in dry environments for months or years Our drugs are also hydrophilic- so they go up and bounce off the cells Hard to identify because they don’t stain, but also because they take a long time to grow- days to double 2-3 weeks to see if you can stain them
37
What is the Periplasmic space?
Between outer membrane and cell membrane Includes the thin cell wall- Gram -
38
What does the periplasmic space contain?
Contains water, nutrients, and substances secreted by the cell Digestive enzymes Proteins invaded in transport
39
What is an Endospore?
Made by some bacteria Bacilus ( anthrax) Costridium ( c. Diff) Botulism Defense strategy against unfavorable conditions
40
What is the defensive state of an endospore called?
Vegetative cells
41
Explain the process of the endospore
Conditions are not right, Duplicate and replicate their DNA to produce a spore coat with the DNA inside Release from spore coat when conditions are again favourable
42
What are Hami?
Grappling hooks Served in attachment Like fimbriae Several dozen per cell These are only archaea
43
What is the Mitochondria different?
Organelles that have a double membrane- inner and outer membrane phospholipid bilayer Produced most of the cell’s ATP Interior matrix contains 70 C ribosomes Circular molecule of DNA Replicate/ Divide on their own Theory: Mitochondria were an organism in itself (an archaea cell) and through invagination set up shop in another cell Either engulfed Or host-parasite relationship Has its own chromosomes, but does not have all the genes necessary for all the cell’s functions Some of these were moved to the nucleus Can make its own proteins
44
What is Extreme commensalism?
Internal cell lost ability to exist independently Retained own portion of DNA Retained it own ribosomes Retained own cytoplasmic membrane Large cell became dependent on internal cell for aerobic ATP production
45
What are the external structures of eukaryotic cells?
Flagella, cilia, and Glycocalyxes
46
Explain the Glycocalyxes in eukaryotic cells/
Simplee than prokarytotic capsules Function Cell-to cell attachment
47
Explain flagella in eukaryotic cells
Whip like a fish tail as opposed to a propellor like prokaryotic Can only go in one direction In mammals it is the sperm cell
48
Explain cilia in eukaryotic cells
Paramecium Whip and work like a crew boat Pul through the cells through the environment Digestive and respiratory system have cilia Instead of the cell movement, it moves mucus
49
True or false: Animal and protozoal cells do not have cells walls
True
50
Which eukaryotic cells have a cell wall?
Plants, Fungi, algae,
51
True or False: Plants, Fungi, algae cells have a glycocalyx?
False
52
What are the cell walls comprised of in Fungi, algae, and plants?
Plant cells- cellulose Funcalf cell walls, cellulose, chitin and/or glucomannan Algal cell walls= cellulose or agar or carrageenan or silicates or algin or calcicim carbonate or any combination of these
53
What are the functions of the cell wall?
Controls movement into and out of the cell Using diffusion, facilitated diffusion, osmosis, and active transport Performs endocytosis Phagocytosis Pinocytosis Vesicle fuses with lysosome for digestion Exocytosis: Substances are exported from the cell Release proteins, enzymes, and hormones outside of the cell
54
Explain pseudopodia in amoebas.
Moves with its pseudopodia for movement and for feeding Extend them out and wrap around their food- this in then called a food vacuole Then they take up their food