Test 3 Flashcards
(28 cards)
One chain runs _____ and the other strand runs____
5’ to 3’ / 3’to5’
REPLICATION ONLY OCCURS IN THE )______direction
5’-TO-3’
What is Semiconservative replication?
Each replicated DNA mole consiste of one old and one new strand
True or False: DNA replication is an anabolic process?
True
What’s the function of GYRASES AND TOPISOMERASES?
TO REMOVE SUPERCOILS
Which enzyme does the unwinding?
unwinding
TRUE OR FALSE: DNA is methylated
TRUE
What is the Control of gene expression?
Epigenetics
What is a Point mutation?
One base pair is affected (Single polynucleotide polymorphisms)
Silent mutations
Missense mutations:
Nonsense mutation:
Frameshift mutations are
insertions and deletions
Ionizing radiation
Gamma radiation
Infrared/ xray
Nonionizing rations
UV radiation
Stasis/static
Agent or procedure that inhibits, not necessarily kills
Preventing them from reproducing past a certain level
Cide/cidal
Agent or procedure that kills ( inactivates)
Wipes out everything
Phenol coefficient
a number that allows comparison between one disinfectant (or antiseptic) to another
Use-Dilution Test
examines how concentrated a chemical has to be to work
Disk- Diffusion Method
chemicals on a paper disk leak out on a lawn of bacteria
In-Use Tes
chemical is evaluated under “ real world” conditions
Swabs are taken from objects before and after application of disinfectant or antiseptic
To stop microbial growth, an antimicrobial agent must do one and/or two things:
Disrupt the “ cell” integrity
Interfere with the structure of biomolecules
Viruses are acellular
Not made of cells
True or false: Damage to viral envelope interrupts viral replication
True
True or Flase: Naked Viruses are More resistant to treatments and chemicals
True
What are the seven ideal factors for an antimicrobial agent?
Inexpensive
Fast-acting
Stable during storage ( have a long “ half-life)
Effective against most ( if not all) microbial growth
Harmless to humans, animals, and objects
Non-toxic and non-allergenic
Easy to use
Easy to transport
High level germicide
Kills ALL PATHOGENS, INCLUDING ENDOSPORE
Invasive equipment- catheters, impact