Test 3 Flashcards

(28 cards)

1
Q

One chain runs _____ and the other strand runs____

A

5’ to 3’ / 3’to5’

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2
Q

REPLICATION ONLY OCCURS IN THE )______direction

A

5’-TO-3’

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3
Q

What is Semiconservative replication?

A

Each replicated DNA mole consiste of one old and one new strand

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4
Q

True or False: DNA replication is an anabolic process?

A

True

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5
Q

What’s the function of GYRASES AND TOPISOMERASES?

A

TO REMOVE SUPERCOILS

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6
Q

Which enzyme does the unwinding?

A

unwinding

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7
Q

TRUE OR FALSE: DNA is methylated

A

TRUE

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8
Q

What is the Control of gene expression?

A

Epigenetics

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9
Q

What is a Point mutation?

A

One base pair is affected (Single polynucleotide polymorphisms)

Silent mutations
Missense mutations:
Nonsense mutation:

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10
Q

Frameshift mutations are

A

insertions and deletions

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11
Q

Ionizing radiation

A

Gamma radiation
Infrared/ xray

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12
Q

Nonionizing rations

A

UV radiation

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13
Q

Stasis/static

A

Agent or procedure that inhibits, not necessarily kills
Preventing them from reproducing past a certain level

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14
Q

Cide/cidal

A

Agent or procedure that kills ( inactivates)
Wipes out everything

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15
Q

Phenol coefficient

A

a number that allows comparison between one disinfectant (or antiseptic) to another

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16
Q

Use-Dilution Test

A

examines how concentrated a chemical has to be to work

17
Q

Disk- Diffusion Method

A

chemicals on a paper disk leak out on a lawn of bacteria

18
Q

In-Use Tes

A

chemical is evaluated under “ real world” conditions
Swabs are taken from objects before and after application of disinfectant or antiseptic

19
Q

To stop microbial growth, an antimicrobial agent must do one and/or two things:

A

Disrupt the “ cell” integrity
Interfere with the structure of biomolecules

20
Q

Viruses are acellular

A

Not made of cells

21
Q

True or false: Damage to viral envelope interrupts viral replication

22
Q

True or Flase: Naked Viruses are More resistant to treatments and chemicals

23
Q

What are the seven ideal factors for an antimicrobial agent?

A

Inexpensive
Fast-acting
Stable during storage ( have a long “ half-life)
Effective against most ( if not all) microbial growth
Harmless to humans, animals, and objects
Non-toxic and non-allergenic
Easy to use
Easy to transport

24
Q

High level germicide

A

Kills ALL PATHOGENS, INCLUDING ENDOSPORE
Invasive equipment- catheters, impact

25
Intermediate level
Kill fungal spored, protozoan cysts, viruses, and pathogenic bacteria- no endospores Non invasive equipment- endoscopes, respirators
26
Low level
Kill vegetative bacteria, fungi, protozoa, and some viruses Skin contactors- furniture, electrodes
27
What are Psychrophilic microbes?
Refrigerating loving microbes
28
What is Lyophilization?
Freezing with simultaenous drying