Exercise 3: Pipettes Flashcards

1
Q

Pipettes are classified according to:

A

design, drainage characteristic, type

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2
Q

holds or contains a particular volume but does not dispense that exact volume

A

TC ( To contain)

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3
Q

pipette that will dispense the volume indicated

A

TD (to dispense/ deliver)

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4
Q

has continuous etched ring or two small, close continuous rings located near the top of the pipet

A

blowout pipet

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5
Q

pipette where the content drains by gravity.

A

self-draining

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6
Q

the last drop of liquid should be expelled into the receiving vessel

A

blowout pipet

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7
Q

measuring pipette or transfer pipette:

Oswald Folin

A

Transfer

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8
Q

measuring pipette or transfer pipette:

volumetric

A

transfer

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9
Q

measuring pipette or transfer pipette:

Mohr

A

measuring

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10
Q

measuring pipette or transfer pipette:

serologic

A

measuring

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11
Q

measuring pipette or transfer pipette:

pasteur

A

transfer

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12
Q

measuring pipette or transfer pipette:

automatic micropipettes and macropipettes

A

transfer

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13
Q

measuring pipette or transfer pipette:

Bacteriologic

A

measuring

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14
Q

measuring pipette or transfer pipette:

Ball, Kolmer or Kahn

A

measuring

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15
Q

measuring pipette or transfer pipette:

micropipette

A

measuring

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16
Q

can dispense different volumes

A

Measuring graduated pipet

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17
Q

graduated pipette where graduations end above the tip

A

Mohr pipette

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18
Q

measuring pipette that has graduated marks down to the tip

A

serological pipette

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19
Q

the drainage characteristic of Mohr pipette is

A

self-draining

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20
Q

the drainage characteristic of serological pipette is:

A

blowout

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21
Q

which has a larger orifice, serologic or mohr?

A

serologic

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22
Q

It transfers a known volume of liquid without further subdivisions.

A

transfer pipette

23
Q

calibrated to deliver accurately a fixed volume of a dilute aqueous solution.

A

volumetric pipette

24
Q

have bulb closer to the delivery tip

A

Oswald Folin

25
used for accurate measurement of viscous fluids, such as blood or serum
Oswald Folin
26
drainage characteristic of Oswald Folin:
blowout
27
drainage characteristic of Volumetric pipetee
self-draining
28
use of Oswald Folin
biologic fluids with greater viscosity than water
29
pipettes that do not have calibration marks and are used to transfer solutions or biologic fluids without consideration of a specific volume and should not be used in any quantitative analytic techniques.
Pasteur pipettes
30
most routinely used pipet in today’s clinical chemistry laboratory.
automatic pipette
31
parts of an automatic pipette: | Used to take up and expel liquid
Plunger
32
parts of an automatic pipette: | pushes the metal bar down which pushes the pipette tip off
eject button
33
parts of an automatic pipette: | rotated to increase and decrease set volume
volume adjustment
34
parts of an automatic pipette: | displays set volume and place values shown depending on pipette size
volume window
35
parts of an automatic pipette: | highest place value which corresponds to size of pipette
top number
36
parts of an automatic pipette: | lowest place value representing the smallesr you can accurately measure (significant figure)
dashes
37
parts of an automatic pipette: | tube filled with air or sample liquid
pipette barrel
38
parts of an automatic pipette: | the first stop is for ______
taking up liquid (set volume
39
parts of an automatic pipette: | the second stop is for ________
forcing out liquid (extra volume)
40
parts of an automatic pipette: | sized to fit a specific pipette and changes everytime there's a risk of contamination
pipette tip
41
automatic pipettes are clasisfied based on:
delivery volume amount total volume capacity mechanism
42
automatic pipette that dispenses only one volume
fixed volume
43
pipette models capable of dispensing different volumes
variable volume
44
automatic pipet capability of less than 1 mL
automaticc micropipet
45
automatic pipet that dispenses greater than 1 mL
automatic macropipet
46
Automatic pipette that relies on a piston for creating suction to draw the sample into a disposable tip that must be changed after each use. The piston does not come in contact with the solution
Air-displacement pipette
47
a method used to compare the reproducibility of the different brands of pipet tips uses
0.1% solution of phenol red in distilled water
48
Operates by moving the piston in the pipet tip or barrel, much like a hypodermic syringe, and does not require a different tip for each use (must be rinsed and blotted between samples)
positive displacement
49
Automatic pipets that obtain the liquid from a common reservoir and dispense it repeatedly
Dilutors/ Dispensers
50
what pipette to use when: | measuring a volume of 7ml
10 ml
51
what pipette to use when: | measurring a volume of 3.5 ml
5 ml
52
what pipette to use when: | measuring a volume of 1.7 ml
2ml
53
what pipette to use when: | measuring a volume of 0.5 ml
1 ml
54
immerse the pipette into the solution with the delivery tip about _______ deep
1 inch